##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062226_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1400539
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.238982277537435	32.0	32.0	32.0	32.0	32.0
2	30.743021079741442	32.0	32.0	32.0	32.0	32.0
3	30.760711411820736	32.0	32.0	32.0	32.0	32.0
4	30.775599965441877	32.0	32.0	32.0	32.0	32.0
5	30.68314698840946	32.0	32.0	32.0	32.0	32.0
6	34.341452826376134	36.0	36.0	36.0	32.0	36.0
7	34.27191388458301	36.0	36.0	36.0	32.0	36.0
8	34.227428868457075	36.0	36.0	36.0	32.0	36.0
9	34.377744568341186	36.0	36.0	36.0	32.0	36.0
10	34.032511054672526	36.0	36.0	36.0	32.0	36.0
11	34.370349558277205	36.0	36.0	36.0	32.0	36.0
12	34.16547129355198	36.0	36.0	36.0	32.0	36.0
13	34.26191130700395	36.0	36.0	36.0	32.0	36.0
14	34.1586689124687	36.0	36.0	36.0	32.0	36.0
15	34.10159945563815	36.0	36.0	36.0	32.0	36.0
16	34.111341419267866	36.0	36.0	36.0	32.0	36.0
17	34.03619606451516	36.0	36.0	36.0	32.0	36.0
18	34.04601585532427	36.0	36.0	36.0	32.0	36.0
19	34.05748358310622	36.0	36.0	36.0	32.0	36.0
20	34.04519759892442	36.0	36.0	36.0	32.0	36.0
21	34.02310539013908	36.0	36.0	36.0	32.0	36.0
22	33.99397017862409	36.0	36.0	36.0	32.0	36.0
23	33.959247832441655	36.0	36.0	36.0	32.0	36.0
24	33.93114079650763	36.0	36.0	36.0	32.0	36.0
25	33.578945677342794	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	20.0
5	93.0
6	297.0
7	67.0
8	246.0
9	213.0
10	96.0
11	31.0
12	64.0
13	57.0
14	265.0
15	373.0
16	537.0
17	736.0
18	976.0
19	1300.0
20	1971.0
21	2883.0
22	4236.0
23	6270.0
24	8956.0
25	12416.0
26	16915.0
27	21874.0
28	28648.0
29	37892.0
30	49663.0
31	67513.0
32	95397.0
33	134637.0
34	291610.0
35	614285.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.93631344111626	17.56333847662065	11.952966410597124	25.54738167166596
2	16.77294748078413	19.22443923215057	37.17700735790251	26.825605929162784
3	19.134409692372284	22.636456069299776	28.319757462207	29.90937677612094
4	12.996892325357539	15.382846664248317	34.85208554102959	36.76817546936456
5	15.24563971033063	36.04732304391395	33.0082802470432	15.698756998712216
6	35.238993441076865	34.569811806061644	16.25419756791129	13.9369971849502
7	30.958512835138535	30.194401616570566	19.806777788023112	19.040307760267787
8	28.522107592643025	32.99566332785084	18.70866240790737	19.773566671598765
9	26.863674284043544	14.709803604784744	18.689035309390555	39.737486801781166
10	16.844206532999966	26.637772199394703	30.340191598630966	26.17782966897436
11	37.10054999899896	21.405557764195436	21.64659346693857	19.847298769867034
12	24.398260326185266	23.69243836748643	28.19820212733251	23.71109917899579
13	29.230450262378998	19.44106839012254	25.056050445400864	26.272430902097604
14	23.68742543306901	19.519540556904623	24.774556780238342	32.018477229788026
15	25.245487421406132	27.04544252083801	21.912335215629973	25.796734842125886
16	25.974740842357953	25.982818854509283	23.10861923896547	24.933821064167297
17	24.158488181230663	26.208900251073786	24.836072839993363	24.79653872770219
18	25.028932351551482	25.22171938585499	25.590137767315984	24.159210495277538
19	25.641505779501976	25.178588147679086	25.153215944607123	24.02669012821182
20	25.824551623588942	24.478725571123807	24.58449916560357	25.112223639683677
21	26.529875680292164	24.310131037760456	24.49359104926691	24.66640223268047
22	26.117420858141482	24.128814140822115	24.84207366321914	24.911691337817267
23	24.52826548193206	24.335462644628926	25.307909275474376	25.828362597964638
24	24.726276068095082	24.93782250110776	25.235845685453324	25.100055745343834
25	24.92789128091956	24.480945860573613	25.12156286536752	25.46959999313931
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	485.0
1	485.0
2	502.5
3	520.0
4	520.0
5	520.0
6	1040.5
7	1561.0
8	1561.0
9	1561.0
10	1942.0
11	2323.0
12	2323.0
13	2323.0
14	2946.0
15	3569.0
16	3569.0
17	3569.0
18	6103.0
19	8637.0
20	8637.0
21	8637.0
22	13449.0
23	18261.0
24	18261.0
25	18261.0
26	26842.5
27	35424.0
28	35424.0
29	35424.0
30	44561.0
31	53698.0
32	53698.0
33	53698.0
34	67889.5
35	82081.0
36	82081.0
37	82081.0
38	96389.5
39	110698.0
40	110698.0
41	110698.0
42	129023.5
43	147349.0
44	147349.0
45	147349.0
46	164371.5
47	181394.0
48	181394.0
49	181394.0
50	187603.5
51	193813.0
52	193813.0
53	193813.0
54	181785.5
55	169758.0
56	169758.0
57	169758.0
58	156577.0
59	143396.0
60	143396.0
61	143396.0
62	126181.0
63	108966.0
64	108966.0
65	108966.0
66	88864.0
67	68762.0
68	68762.0
69	68762.0
70	51916.5
71	35071.0
72	35071.0
73	35071.0
74	26186.0
75	17301.0
76	17301.0
77	17301.0
78	13832.5
79	10364.0
80	10364.0
81	10364.0
82	7108.5
83	3853.0
84	3853.0
85	3853.0
86	2869.0
87	1885.0
88	1885.0
89	1885.0
90	1258.5
91	632.0
92	632.0
93	632.0
94	406.5
95	181.0
96	181.0
97	181.0
98	369.0
99	557.0
100	557.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.712086560959744E-4
2	2.856043280479872E-4
3	0.0010710162301799523
4	0.009639146071619569
5	0.03284449772551853
6	0.06561759436902506
7	0.10910085331433113
8	0.1099576662984751
9	0.11966821345210665
10	0.134662440674626
11	0.14215955428588564
12	0.134662440674626
13	0.12845054653958227
14	0.1162409615155308
15	0.13516224824870995
16	0.11995381778015465
17	0.12473769027495842
18	0.11324211607102692
19	0.09781948235643562
20	0.09453503258388378
21	0.09453503258388378
22	0.10488818947562331
23	0.08439607893818023
24	0.0941780271738238
25	0.09017956658115196
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1400539.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.84112593780978
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.2730744777511	41.06628208620541
2	13.844279078654962	14.907831467840937
3	4.420300043736948	7.139817940132414
4	1.851841176632264	3.9882085603151824
5	0.9566109094652269	2.5752504225000012
6	0.5803295736517202	1.8747358596250634
7	0.40309506856027605	1.5192164645884723
8	0.2617382770485646	1.1273826829853693
9	0.215999913425547	1.0466710687170797
>10	1.0309669626463867	9.90226842825072
>50	0.08176956849926734	3.0837506007938438
>100	0.07134329658961502	7.8087290011435275
>500	0.006655117952385809	2.4715414365520108
>1k	0.001996535385715743	1.4883139803500394
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2684	0.19164050412019945	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1865	0.13316301795237406	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1503	0.10731582626403122	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.428021640239936E-4	0.0
10	0.0	0.0	0.0	1.428021640239936E-4	0.0
11	0.0	0.0	0.0	1.428021640239936E-4	0.0
12	0.0	0.0	0.0	1.428021640239936E-4	0.0
13	0.0	7.14010820119968E-5	0.0	2.1420324603599043E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACCGT	45	6.754496E-4	14.779864	6
CGTCTTA	75	9.674823E-7	13.932809	15
CGTCGTA	200	0.0	12.827269	10
CGGTCCA	225	0.0	12.668907	10
ACCGTCC	75	1.4761876E-5	12.668455	8
GGTATCA	800	0.0	12.46427	1
TCGCGTA	140	5.2750693E-11	12.216884	9
GTATCAA	1740	0.0	12.170912	1
GGTAGCG	55	0.0030649395	12.092616	7
CGAACGA	55	0.0030706914	12.089588	16
CGCGTAA	145	9.640644E-11	11.795191	10
ACGGTAT	195	0.0	11.694795	9
CGTTATT	155	2.5465852E-11	11.642225	2
CGAACGT	90	7.45911E-6	11.611089	4
ATCCCGA	90	7.468023E-6	11.609843	16
CCGTCAA	205	0.0	11.58493	18
GGGTAGG	390	0.0	11.444604	1
CCGTCGT	225	0.0	11.402424	9
CTAGGGT	100	1.9289764E-6	11.399978	4
CGGAATT	75	2.0742565E-4	11.399571	15
>>END_MODULE