FastQCFastQC Report
Thu 2 Feb 2017
SRR4062226_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062226_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400539
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14800.10567360137775528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19000.026.405421
GGTATCA9600.023.8382281
CGACGGT3400.018.1161927
ACGACGG3750.017.5985876
TACCGTC3200.017.186127
GTCCTAT3750.017.0168271
TACGACG3750.017.0119675
GTATTAC1702.4610927E-916.8269841
TATCAAC29350.016.789112
CGCAATA3550.016.73224436
TCAACGC29000.016.6883144
GGTCTAG1206.395494E-616.5033871
CAACGCA29200.016.4986745
ACCGTCG3200.016.4986748
ATCAACG29350.016.4893053
ATACGAA3750.016.4300440
AACGCAG29550.016.3032596
GTATTAT1906.148184E-1016.2138561
GGACCGT1501.8398714E-716.1320386
CTAATGT1105.6261313E-515.9987143
Produced by FastQC (version 0.11.5)