##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062225_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10767
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.981610476455838	32.0	14.0	32.0	14.0	32.0
2	24.713290610197827	32.0	14.0	32.0	14.0	32.0
3	25.154824928020805	32.0	14.0	32.0	14.0	32.0
4	24.700659422308906	32.0	14.0	32.0	14.0	32.0
5	25.059440884183154	32.0	14.0	32.0	14.0	32.0
6	28.520014860221046	32.0	21.0	36.0	14.0	36.0
7	28.042908888269714	32.0	21.0	36.0	14.0	36.0
8	27.690535896721464	32.0	21.0	36.0	14.0	36.0
9	27.822606111265905	32.0	14.0	36.0	14.0	36.0
10	26.440884183152225	32.0	14.0	36.0	14.0	36.0
11	28.68839973994613	32.0	21.0	36.0	14.0	36.0
12	27.23683477291725	32.0	14.0	36.0	14.0	36.0
13	27.92300547970651	32.0	21.0	36.0	14.0	36.0
14	27.104114423702054	32.0	14.0	36.0	14.0	36.0
15	27.217980867465403	32.0	14.0	36.0	14.0	36.0
16	27.408841831522246	32.0	21.0	36.0	14.0	36.0
17	27.157704095848427	32.0	14.0	36.0	14.0	36.0
18	27.19476177208136	32.0	14.0	36.0	14.0	36.0
19	27.17748676511563	32.0	14.0	36.0	14.0	36.0
20	27.239342435218724	32.0	14.0	36.0	14.0	36.0
21	27.15658957926999	32.0	14.0	36.0	14.0	36.0
22	26.990433732701774	32.0	14.0	36.0	14.0	36.0
23	26.785269805888362	32.0	14.0	36.0	14.0	36.0
24	26.621993127147768	32.0	14.0	36.0	14.0	36.0
25	25.905916225503855	32.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	1.0
5	1.0
6	2.0
7	1.0
8	2.0
9	0.0
10	2.0
11	0.0
12	0.0
13	1.0
14	24.0
15	52.0
16	131.0
17	247.0
18	419.0
19	604.0
20	715.0
21	636.0
22	640.0
23	554.0
24	470.0
25	518.0
26	441.0
27	447.0
28	439.0
29	417.0
30	461.0
31	537.0
32	568.0
33	624.0
34	918.0
35	894.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.1132163090926	17.74867651156311	11.41450729079595	27.723599888548346
2	17.804402340484813	18.73316615584657	38.47868487043745	24.983746633231167
3	18.361660629701866	21.277979009937773	29.6089904337327	30.75136992662766
4	13.107292150487693	14.788666976312124	36.042731072921505	36.06130980027868
5	15.506828951035956	34.91591563690421	34.30270370714485	15.274551704914987
6	34.8359512965889	34.45487498838182	16.14462310623664	14.56455060879264
7	29.337920773665616	30.184117537660406	20.755067881718432	19.72289380695555
8	27.382612738261276	33.14737331473733	19.581589958158997	19.888423988842398
9	26.225239468055427	13.289314609876312	18.81335441272203	41.672091509346224
10	14.797247023809524	27.297247023809522	32.29166666666667	25.613839285714285
11	36.65364583333333	20.554315476190478	22.637648809523807	20.15438988095238
12	23.674911660777383	23.66561279523898	29.709875395201784	22.949600148781848
13	29.151943462897528	19.825181327878	25.181327877998882	25.84154733122559
14	22.35447275432397	18.709317463269482	26.901618002603684	32.03459177980286
15	24.423363095238095	26.739211309523807	23.074776785714285	25.762648809523807
16	24.600223131275566	25.743770918557086	24.312011900334696	25.343994049832652
17	23.28932688731871	26.3015991074749	25.539233915953886	24.86984008925251
18	23.693509391854196	25.08833922261484	26.929514599218894	24.288636786312072
19	24.900046490004648	25.327754532775455	25.913528591352858	23.85867038586704
20	24.2796058746979	24.335378323108383	26.68711656441718	24.69789923777654
21	24.702491632577168	23.53105243584976	26.62699888434362	25.139457047229453
22	24.823354406842693	23.77277798438081	26.552621792487912	24.851245816288582
23	23.259273031514365	23.807753091010504	26.67100492702426	26.26196895045087
24	23.387246700130135	23.70329057445622	27.765383900353225	25.14407882506042
25	24.079583488285607	23.884343622164373	26.943101524730384	25.092971364819633
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	4.0
3	8.0
4	8.0
5	8.0
6	12.5
7	17.0
8	17.0
9	17.0
10	20.5
11	24.0
12	24.0
13	24.0
14	38.0
15	52.0
16	52.0
17	52.0
18	79.0
19	106.0
20	106.0
21	106.0
22	151.5
23	197.0
24	197.0
25	197.0
26	276.0
27	355.0
28	355.0
29	355.0
30	428.5
31	502.0
32	502.0
33	502.0
34	617.5
35	733.0
36	733.0
37	733.0
38	769.5
39	806.0
40	806.0
41	806.0
42	951.5
43	1097.0
44	1097.0
45	1097.0
46	1200.5
47	1304.0
48	1304.0
49	1304.0
50	1348.5
51	1393.0
52	1393.0
53	1393.0
54	1305.0
55	1217.0
56	1217.0
57	1217.0
58	1138.0
59	1059.0
60	1059.0
61	1059.0
62	938.5
63	818.0
64	818.0
65	818.0
66	656.0
67	494.0
68	494.0
69	494.0
70	401.5
71	309.0
72	309.0
73	309.0
74	228.5
75	148.0
76	148.0
77	148.0
78	108.0
79	68.0
80	68.0
81	68.0
82	47.0
83	26.0
84	26.0
85	26.0
86	21.5
87	17.0
88	17.0
89	17.0
90	10.0
91	3.0
92	3.0
93	3.0
94	2.5
95	2.0
96	2.0
97	2.0
98	7.0
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.01857527630723507
5	0.03715055261447014
6	0.07430110522894028
7	0.12073929599702796
8	0.11145165784341043
9	0.1300269341506455
10	0.13931457230426303
11	0.13931457230426303
12	0.12073929599702796
13	0.12073929599702796
14	0.12073929599702796
15	0.13931457230426303
16	0.10216401968979288
17	0.10216401968979288
18	0.12073929599702796
19	0.11145165784341043
20	0.08358874338255781
21	0.10216401968979288
22	0.10216401968979288
23	0.09287638153617535
24	0.08358874338255781
25	0.10216401968979288
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	10767.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.87944645676605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.09220419469727	90.21082938608713
2	3.1064503363672338	5.832636760471812
3	0.4352987732489117	1.2259682362775146
4	0.16818361693707953	0.6315593944459924
5	0.07914523149980214	0.3715055261447014
6	0.0296794618124258	0.16717748676511562
7	0.0	0.0
8	0.009893153937475268	0.07430110522894028
9	0.0296794618124258	0.25076623014767346
>10	0.049465769687376336	1.2352558744311322
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	48	0.44580663137364174	No Hit
TATCAACGCAGAGTACTTTTTTTTT	30	0.27862914460852606	No Hit
GGTATCAACGCAGAGTACTTTTTTT	21	0.19504040122596825	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	21	0.19504040122596825	No Hit
TTCTTATACATCTCCTCCATCATGT	13	0.12073929599702796	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE