##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062225_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10767
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.121760936193926	32.0	32.0	32.0	21.0	32.0
2	28.7548063527445	32.0	32.0	32.0	14.0	32.0
3	30.36890498746169	32.0	32.0	32.0	21.0	32.0
4	30.968979288566917	32.0	32.0	32.0	32.0	32.0
5	30.612798365375685	32.0	32.0	32.0	32.0	32.0
6	34.1545462988762	36.0	36.0	36.0	32.0	36.0
7	34.123989969350795	36.0	36.0	36.0	32.0	36.0
8	33.96340670567475	36.0	36.0	36.0	32.0	36.0
9	34.28067242500232	36.0	36.0	36.0	32.0	36.0
10	33.81183245100771	36.0	36.0	36.0	32.0	36.0
11	34.54258382093434	36.0	36.0	36.0	32.0	36.0
12	34.181201820377076	36.0	36.0	36.0	32.0	36.0
13	34.35413764279744	36.0	36.0	36.0	32.0	36.0
14	34.25680319494752	36.0	36.0	36.0	32.0	36.0
15	34.07142193740132	36.0	36.0	36.0	32.0	36.0
16	34.21296554286245	36.0	36.0	36.0	32.0	36.0
17	34.125475991455374	36.0	36.0	36.0	32.0	36.0
18	34.246679669360084	36.0	36.0	36.0	32.0	36.0
19	34.172099934986534	36.0	36.0	36.0	32.0	36.0
20	34.14395839138107	36.0	36.0	36.0	32.0	36.0
21	34.10522894028048	36.0	36.0	36.0	32.0	36.0
22	34.079502182594965	36.0	36.0	36.0	32.0	36.0
23	34.10912974830501	36.0	36.0	36.0	32.0	36.0
24	33.960063155939444	36.0	36.0	36.0	32.0	36.0
25	34.031949475248446	36.0	36.0	36.0	32.0	36.0
26	33.862635831708	36.0	36.0	36.0	32.0	36.0
27	33.77180273056562	36.0	36.0	36.0	32.0	36.0
28	33.87712454722764	36.0	36.0	36.0	32.0	36.0
29	33.73409491966193	36.0	36.0	36.0	32.0	36.0
30	33.76975945017182	36.0	36.0	36.0	32.0	36.0
31	33.68774960527538	36.0	36.0	36.0	27.0	36.0
32	33.7537847125476	36.0	36.0	36.0	32.0	36.0
33	33.589672146373175	36.0	36.0	36.0	27.0	36.0
34	33.58057026098263	36.0	36.0	36.0	27.0	36.0
35	33.51221324417201	36.0	36.0	36.0	21.0	36.0
36	33.507662301476735	36.0	36.0	36.0	21.0	36.0
37	33.55354323395561	36.0	36.0	36.0	27.0	36.0
38	33.41450729079595	36.0	36.0	36.0	21.0	36.0
39	33.45936658307792	36.0	36.0	36.0	21.0	36.0
40	33.41172099934987	36.0	36.0	36.0	21.0	36.0
41	33.30138385808489	36.0	36.0	36.0	21.0	36.0
42	33.301755363611036	36.0	36.0	36.0	21.0	36.0
43	33.22466796693601	36.0	36.0	36.0	21.0	36.0
44	33.20544255595802	36.0	36.0	36.0	21.0	36.0
45	33.21844524937308	36.0	36.0	36.0	21.0	36.0
46	33.11609547692022	36.0	36.0	36.0	21.0	36.0
47	33.070307420822886	36.0	36.0	36.0	14.0	36.0
48	33.01978266926721	36.0	36.0	36.0	14.0	36.0
49	33.05851212036779	36.0	36.0	36.0	14.0	36.0
50	32.262097148695084	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	2.0
23	2.0
24	15.0
25	28.0
26	92.0
27	128.0
28	201.0
29	364.0
30	556.0
31	903.0
32	1322.0
33	1854.0
34	2799.0
35	2501.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.28039379585771	17.832265254945668	12.863378842760287	26.023962106436333
2	18.493405164406465	19.95169979565298	35.714285714285715	25.84060932565484
3	22.9869044302034	22.336769759450174	28.03937958577134	26.63694622457509
4	16.374106064827714	15.779697222996191	36.47255502925606	31.373641682920034
5	20.80430946410328	32.34884368904987	31.095012538311508	15.75183430853534
6	35.92458437819263	35.385901365282805	14.925234512863378	13.764279743661186
7	29.571839881118233	30.463453143865515	19.355437912138942	20.60926906287731
8	28.345871644840713	33.07327946503204	18.08303148509334	20.4978174050339
9	26.78554843503297	13.11414507290796	16.559858827900065	43.540447664159004
10	14.191511098727593	26.41404290888827	32.2559673075137	27.13847868487044
11	39.24027119903409	19.457601931828737	21.324417200705863	19.977709668431316
12	25.55029256060184	21.73307327946503	28.355159282994336	24.361474876938793
13	30.268412742639544	19.048945853069565	23.646326739110243	27.036314665180644
14	23.943531160026005	18.073743846939724	24.389337791399647	33.59338720163462
15	25.494566731680134	26.10755084981889	21.25940373363054	27.13847868487044
16	25.42955326460481	25.42955326460481	23.02405498281787	26.11683848797251
17	24.15714683755921	24.463638896628588	24.965171356923936	26.41404290888827
18	25.271663415993313	25.336676883068638	24.064270456023035	25.327389244915015
19	25.819634067056747	25.067335376613727	24.667966936008174	24.445063620321353
20	25.977523915668243	24.08284573233027	24.60295346893285	25.336676883068638
21	25.141636481842667	24.15714683755921	24.667966936008174	26.03324974458995
22	25.05804773846011	24.86300733723414	24.342899600631558	25.73604532367419
23	25.73604532367419	24.983746633231167	23.73920312064642	25.541004922448224
24	24.93730844246308	25.290238692300548	23.460573976037892	26.311878889198475
25	24.361474876938793	24.361474876938793	25.893935172285687	25.38311507383672
26	25.048760100306495	24.779418593851585	23.943531160026005	26.22829014581592
27	24.81656914646605	25.67103185659887	24.463638896628588	25.048760100306495
28	23.85994241664345	24.983746633231167	25.02089718584564	26.13541376427974
29	24.72369276492988	25.791771152595892	23.952818798179624	25.531717284294604
30	24.946596080616697	25.280951054146932	24.500789449243058	25.271663415993313
31	25.308813968607787	25.076623014767346	24.85371969908052	24.76084331754435
32	25.940373363053777	24.584378192625618	24.027119903408565	25.448128540912045
33	25.253088139686074	25.801058790749515	23.655614377263863	25.290238692300548
34	24.686542212315405	25.160211758149902	25.02089718584564	25.13234884368905
35	25.513142007987366	25.50385436983375	24.630816383393704	24.352187238785177
36	24.87229497538776	25.095198291074578	24.64010402154732	25.392402711990343
37	25.866072257824836	24.918733166155846	24.370762515092412	24.84443206092691
38	25.606018389523545	24.56580291631838	24.38005015324603	25.448128540912045
39	25.215937587071608	25.42955326460481	23.80421658772174	25.55029256060184
40	24.93730844246308	25.45741617906566	23.96210643633324	25.64316894213801
41	24.948913245402192	25.766301318967116	24.660969719487273	24.623815716143415
42	25.085910652920962	25.606018389523545	23.85065477848983	25.45741617906566
43	25.095198291074578	25.55029256060184	24.881582613541376	24.472926534782204
44	26.10755084981889	25.002321909538406	24.0456951797158	24.84443206092691
45	25.262375777839697	25.52242964614099	24.1014210086375	25.113773567381813
46	24.946596080616697	25.215937587071608	24.76084331754435	25.076623014767346
47	25.12306120553543	24.881582613541376	24.93730844246308	25.05804773846011
48	24.716700724503067	25.431915288872375	24.363737692736393	25.487646293888165
49	24.500789449243058	24.72369276492988	24.90015788984861	25.875359895978452
50	25.450492290544307	25.088240757941666	24.150102173509197	25.31116477800483
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	2.5
4	4.0
5	3.0
6	2.0
7	1.0
8	0.0
9	1.0
10	2.0
11	3.5
12	5.0
13	9.0
14	13.0
15	16.0
16	19.0
17	21.5
18	24.0
19	24.0
20	24.0
21	44.0
22	64.0
23	66.5
24	69.0
25	89.0
26	109.0
27	121.5
28	134.0
29	164.5
30	195.0
31	216.0
32	237.0
33	250.0
34	263.0
35	277.5
36	292.0
37	333.5
38	375.0
39	400.5
40	426.0
41	457.0
42	488.0
43	490.0
44	492.0
45	526.0
46	560.0
47	592.0
48	624.0
49	630.0
50	636.0
51	662.0
52	688.0
53	709.5
54	731.0
55	719.5
56	708.0
57	699.5
58	691.0
59	668.5
60	646.0
61	604.0
62	562.0
63	496.0
64	430.0
65	384.0
66	338.0
67	300.0
68	262.0
69	222.5
70	183.0
71	167.0
72	151.0
73	127.5
74	104.0
75	92.0
76	80.0
77	67.0
78	54.0
79	46.0
80	38.0
81	26.5
82	15.0
83	13.0
84	11.0
85	10.0
86	9.0
87	5.5
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.009287638153617534
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.009287638153617534
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.009287638153617534
49	0.0
50	0.009287638153617534
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	10767.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.56357388316151
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.2395883427912	93.89802173307328
2	2.2698855439068963	4.3837652085074765
3	0.30778109069924015	0.8916132627472835
4	0.12503606809656634	0.4829571839881118
5	0.01923631816870251	0.09287638153617535
6	0.028854477253053765	0.16717748676511562
7	0.0	0.0
8	0.0	0.0
9	0.009618159084351255	0.08358874338255781
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.009287638153617534	0.0
35	0.0	0.0	0.0	0.009287638153617534	0.0
36	0.0	0.0	0.0	0.009287638153617534	0.0
37	0.0	0.0	0.0	0.009287638153617534	0.0
38	0.0	0.0	0.0	0.009287638153617534	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE