FastQCFastQC Report
Thu 2 Feb 2017
SRR4062224_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062224_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences945878
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23240.24569764811106717No Hit
TATCAACGCAGAGTACTTTTTTTTT12750.13479539644647617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA504.556183E-617.1009069
GTATTAG653.3798624E-614.61074351
CAAGACG1301.8189894E-1213.8846154
CGCAAGA1301.8189894E-1213.8831452
AAGACGG1351.8189894E-1213.3703715
GCGTAAC655.4426615E-513.15523911
CCGCTAG604.087558E-412.6673379
TAGTACT604.0893842E-412.6666674
TTTTCGG751.4779336E-512.66599716
GGGTCGC853.9363604E-612.2947686
CCGACCA853.9363604E-612.2947689
TTAGGAC1650.012.0902693
TTAAGGG550.003068130712.0902693
ATTTGCG550.003068130712.09026916
CGGACCA1353.6925485E-1011.9635969
AGGATAG802.8635724E-511.8755
CTATTAA802.8728957E-511.871231
GCCGCTA658.014749E-411.6929268
GTATCAA13550.011.4945041
GTCGCGT752.0711542E-411.4006048
Produced by FastQC (version 0.11.5)