##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062224_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	945878
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26678176255289	32.0	32.0	32.0	32.0	32.0
2	30.92754456705833	32.0	32.0	32.0	32.0	32.0
3	30.94919640799342	32.0	32.0	32.0	32.0	32.0
4	30.94624042424076	32.0	32.0	32.0	32.0	32.0
5	30.911132302474527	32.0	32.0	32.0	32.0	32.0
6	34.546465823287996	36.0	36.0	36.0	32.0	36.0
7	34.47229769589735	36.0	36.0	36.0	32.0	36.0
8	34.44098921848272	36.0	36.0	36.0	32.0	36.0
9	34.52415216338682	36.0	36.0	36.0	32.0	36.0
10	34.30133590167019	36.0	36.0	36.0	32.0	36.0
11	34.520765891584325	36.0	36.0	36.0	32.0	36.0
12	34.38338453796367	36.0	36.0	36.0	32.0	36.0
13	34.44743613869865	36.0	36.0	36.0	32.0	36.0
14	34.3790435975887	36.0	36.0	36.0	32.0	36.0
15	34.35027561694003	36.0	36.0	36.0	32.0	36.0
16	34.34665252812731	36.0	36.0	36.0	32.0	36.0
17	34.28625150389374	36.0	36.0	36.0	32.0	36.0
18	34.29266776476459	36.0	36.0	36.0	32.0	36.0
19	34.29759228991477	36.0	36.0	36.0	32.0	36.0
20	34.254814045786034	36.0	36.0	36.0	32.0	36.0
21	34.244819099291874	36.0	36.0	36.0	32.0	36.0
22	34.22500364740485	36.0	36.0	36.0	32.0	36.0
23	34.203924819057	36.0	36.0	36.0	32.0	36.0
24	34.17492847914848	36.0	36.0	36.0	32.0	36.0
25	33.834985061498415	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	11.0
5	53.0
6	200.0
7	43.0
8	154.0
9	130.0
10	77.0
11	15.0
12	42.0
13	56.0
14	177.0
15	228.0
16	335.0
17	446.0
18	659.0
19	850.0
20	1217.0
21	1689.0
22	2524.0
23	3561.0
24	4986.0
25	6996.0
26	9481.0
27	12210.0
28	16391.0
29	21964.0
30	29208.0
31	39831.0
32	57886.0
33	84117.0
34	191931.0
35	458410.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.40816164708843	17.161893424311977	11.83205178762675	25.59789314097285
2	16.362661794976706	19.32236145867363	38.299539155890905	26.015437590458763
3	19.958852957470114	22.749705035332326	27.652144678583664	29.6392973286139
4	12.766373618240545	15.331595836351045	35.50763635210218	36.394394193306226
5	14.723555912773111	36.57564669303496	33.48135535861588	15.219442035576048
6	34.800412989711226	35.01071615208684	16.52128102976615	13.66758982843578
7	30.51716382763675	30.23924052642441	19.774888476131512	19.468707169807328
8	27.378565970318597	34.05116665996355	18.99540025020268	19.574867119515176
9	26.700326543141273	15.082376725994845	18.34611456001355	39.87118217085033
10	15.774011801845864	27.424788110921263	31.526321249886198	25.27487883734668
11	36.95056515718103	21.704873183725844	21.89608222624074	19.44847943285238
12	24.553506206978327	23.720333438494485	28.914482808268634	22.811677546258558
13	29.4893672880021	19.99159461038796	24.941776268430456	25.57726183317948
14	22.98074124253121	19.872666747815597	25.51332871137267	31.63326329828052
15	24.724271906329733	27.737748652854677	22.346308980615927	25.19167046019966
16	25.034613870105893	26.50702650977868	24.251938165020302	24.20642145509512
17	23.503794434687983	26.527380373949505	25.49178392946554	24.47704126189697
18	23.906442008376526	26.26084508294497	26.313767288361454	23.518945620317048
19	24.985713378627214	25.450928101255105	25.546278070565325	24.017080449552353
20	25.08214294406661	25.30552942384066	25.600238730411355	24.012088901681377
21	25.587212901382518	24.937170309465987	25.289016576456458	24.186600212695037
22	25.034711639405334	25.20752903924955	25.603326391005453	24.154432930339667
23	24.202422570023447	25.35583084332862	25.652938487713627	24.788808098934307
24	24.171928588133085	25.478176121194036	25.8631508883097	24.48674440236318
25	24.48922148338462	25.273536868748863	25.81034636010785	24.426895287758672
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	559.0
1	559.0
2	472.5
3	386.0
4	386.0
5	386.0
6	840.0
7	1294.0
8	1294.0
9	1294.0
10	1542.0
11	1790.0
12	1790.0
13	1790.0
14	2165.5
15	2541.0
16	2541.0
17	2541.0
18	4422.5
19	6304.0
20	6304.0
21	6304.0
22	10072.5
23	13841.0
24	13841.0
25	13841.0
26	20728.0
27	27615.0
28	27615.0
29	27615.0
30	36202.5
31	44790.0
32	44790.0
33	44790.0
34	53693.5
35	62597.0
36	62597.0
37	62597.0
38	72272.0
39	81947.0
40	81947.0
41	81947.0
42	91712.0
43	101477.0
44	101477.0
45	101477.0
46	110859.5
47	120242.0
48	120242.0
49	120242.0
50	123458.0
51	126674.0
52	126674.0
53	126674.0
54	119529.5
55	112385.0
56	112385.0
57	112385.0
58	102214.0
59	92043.0
60	92043.0
61	92043.0
62	79092.0
63	66141.0
64	66141.0
65	66141.0
66	53584.0
67	41027.0
68	41027.0
69	41027.0
70	31069.0
71	21111.0
72	21111.0
73	21111.0
74	16084.5
75	11058.0
76	11058.0
77	11058.0
78	8492.5
79	5927.0
80	5927.0
81	5927.0
82	4137.0
83	2347.0
84	2347.0
85	2347.0
86	1671.0
87	995.0
88	995.0
89	995.0
90	646.5
91	298.0
92	298.0
93	298.0
94	190.0
95	82.0
96	82.0
97	82.0
98	244.5
99	407.0
100	407.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0015858281934879553
2	5.286093978293184E-4
3	2.1144375913172736E-4
4	0.007717697208308049
5	0.03150512011062737
6	0.060895802629937476
7	0.1070962640002199
8	0.11037364226676168
9	0.11978288954812354
10	0.1353240058443055
11	0.14399319996870633
12	0.1370155559173593
13	0.13172946193906615
14	0.11766845195680627
15	0.13616978088083243
16	0.1236945990920605
17	0.12739486487686574
18	0.11576545812462072
19	0.09916712303278012
20	0.09292953213839417
21	0.09123798206534035
22	0.10043578558757049
23	0.08098295974745157
24	0.0931409758975259
25	0.08986359763098412
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	945878.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.89788460081309
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.88273361838083	37.665315121528515
2	16.49656925137227	17.78260371639569
3	6.240287203819704	10.090148387622152
4	2.8907350083251186	6.232180075609509
5	1.4908547107851604	4.017695757923858
6	0.8598191138138158	2.7805458824346263
7	0.5331793041156548	2.011606562333718
8	0.3357598541012493	1.4477396695947982
9	0.2470485688746968	1.1983855730403992
>10	0.910437362180687	8.493866314990353
>50	0.06348833862260188	2.3951488102059755
>100	0.046524855881034326	4.757198125754448
>500	0.00216853130762727	0.7451583467767702
>1k	3.942784195685946E-4	0.38240765578919095
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2324	0.24569764811106717	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1275	0.13479539644647617	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0572187956586368E-4
2	0.0	0.0	0.0	0.0	1.0572187956586368E-4
3	0.0	0.0	0.0	0.0	1.0572187956586368E-4
4	0.0	0.0	0.0	0.0	1.0572187956586368E-4
5	0.0	0.0	0.0	0.0	1.0572187956586368E-4
6	0.0	0.0	0.0	0.0	2.1144375913172736E-4
7	0.0	0.0	0.0	0.0	2.1144375913172736E-4
8	0.0	0.0	0.0	0.0	2.1144375913172736E-4
9	0.0	0.0	0.0	0.0	2.1144375913172736E-4
10	0.0	0.0	0.0	0.0	4.228875182634547E-4
11	0.0	0.0	0.0	0.0	4.228875182634547E-4
12	0.0	0.0	0.0	0.0	8.457750365269094E-4
13	0.0	0.0	0.0	0.0	0.0010572187956586368
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGTA	50	4.556183E-6	17.100906	9
GTATTAG	65	3.3798624E-6	14.6107435	1
CAAGACG	130	1.8189894E-12	13.884615	4
CGCAAGA	130	1.8189894E-12	13.883145	2
AAGACGG	135	1.8189894E-12	13.370371	5
GCGTAAC	65	5.4426615E-5	13.155239	11
CCGCTAG	60	4.087558E-4	12.667337	9
TAGTACT	60	4.0893842E-4	12.666667	4
TTTTCGG	75	1.4779336E-5	12.665997	16
GGGTCGC	85	3.9363604E-6	12.294768	6
CCGACCA	85	3.9363604E-6	12.294768	9
TTAGGAC	165	0.0	12.090269	3
TTAAGGG	55	0.0030681307	12.090269	3
ATTTGCG	55	0.0030681307	12.090269	16
CGGACCA	135	3.6925485E-10	11.963596	9
AGGATAG	80	2.8635724E-5	11.875	5
CTATTAA	80	2.8728957E-5	11.87123	1
GCCGCTA	65	8.014749E-4	11.692926	8
GTATCAA	1355	0.0	11.494504	1
GTCGCGT	75	2.0711542E-4	11.400604	8
>>END_MODULE