FastQCFastQC Report
Thu 2 Feb 2017
SRR4062224_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062224_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences945878
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11110.11745700819767455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16300.024.1663021
GATATAC3650.023.513461
TAGGACG2300.021.042724
GTCCTAC3250.020.3134021
CGATTAA550.004483837619.9992827
AACGACT550.004483837619.9992818
GTAGGAC3950.019.492973
AATCCCG2950.019.38913219
GTCCTAA1404.0636223E-918.8624481
GTACTAG708.1061956E-418.8624481
GGAATAT4900.018.85646212
TACACCG708.123505E-418.8564625
AACGCAG21300.018.694166
CAACGTA951.5970068E-518.52564820
TGTAGGA5050.018.2963712
ATCAACG22350.017.9143453
ATACACT5050.017.8607434
GGACGTG2850.017.7537466
ATCCCGT3150.017.45968820
TCCCGTT2900.017.44764721
Produced by FastQC (version 0.11.5)