##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062224_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	945878
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.265472925683863	32.0	32.0	32.0	32.0	32.0
2	31.451060284730165	32.0	32.0	32.0	32.0	32.0
3	31.57083577374672	32.0	32.0	32.0	32.0	32.0
4	31.65235474342357	32.0	32.0	32.0	32.0	32.0
5	31.620737558120602	32.0	32.0	32.0	32.0	32.0
6	35.26525090973677	36.0	36.0	36.0	36.0	36.0
7	35.26736429010929	36.0	36.0	36.0	36.0	36.0
8	35.221399588530446	36.0	36.0	36.0	36.0	36.0
9	35.29186850735507	36.0	36.0	36.0	36.0	36.0
10	35.18141874533502	36.0	36.0	36.0	36.0	36.0
11	35.29843489329491	36.0	36.0	36.0	36.0	36.0
12	35.231283527051055	36.0	36.0	36.0	36.0	36.0
13	35.25575179885778	36.0	36.0	36.0	36.0	36.0
14	35.22896716066977	36.0	36.0	36.0	36.0	36.0
15	35.200063856015255	36.0	36.0	36.0	36.0	36.0
16	35.21730920901004	36.0	36.0	36.0	36.0	36.0
17	35.19631707260344	36.0	36.0	36.0	36.0	36.0
18	35.19826869850023	36.0	36.0	36.0	36.0	36.0
19	35.18407130729333	36.0	36.0	36.0	36.0	36.0
20	35.16816756495024	36.0	36.0	36.0	36.0	36.0
21	35.160210936294106	36.0	36.0	36.0	36.0	36.0
22	35.14902027534206	36.0	36.0	36.0	36.0	36.0
23	35.122421707662085	36.0	36.0	36.0	36.0	36.0
24	35.117647307580896	36.0	36.0	36.0	36.0	36.0
25	35.08857379070028	36.0	36.0	36.0	36.0	36.0
26	35.053683456005956	36.0	36.0	36.0	36.0	36.0
27	35.04888368267366	36.0	36.0	36.0	36.0	36.0
28	35.02827531668989	36.0	36.0	36.0	36.0	36.0
29	35.00344441883625	36.0	36.0	36.0	36.0	36.0
30	34.98141197913473	36.0	36.0	36.0	36.0	36.0
31	34.979537530210024	36.0	36.0	36.0	36.0	36.0
32	34.947927745438626	36.0	36.0	36.0	36.0	36.0
33	34.92747056174264	36.0	36.0	36.0	36.0	36.0
34	34.91043876694457	36.0	36.0	36.0	36.0	36.0
35	34.89225671809684	36.0	36.0	36.0	32.0	36.0
36	34.871728700741535	36.0	36.0	36.0	32.0	36.0
37	34.86266199234996	36.0	36.0	36.0	32.0	36.0
38	34.83775814639943	36.0	36.0	36.0	32.0	36.0
39	34.81292090523302	36.0	36.0	36.0	32.0	36.0
40	34.79661541974758	36.0	36.0	36.0	32.0	36.0
41	34.77212177468976	36.0	36.0	36.0	32.0	36.0
42	34.749429630459744	36.0	36.0	36.0	32.0	36.0
43	34.72755577357756	36.0	36.0	36.0	32.0	36.0
44	34.67905903298311	36.0	36.0	36.0	32.0	36.0
45	34.66142673790912	36.0	36.0	36.0	32.0	36.0
46	34.615871180004184	36.0	36.0	36.0	32.0	36.0
47	34.584797405162185	36.0	36.0	36.0	32.0	36.0
48	34.55316964767126	36.0	36.0	36.0	32.0	36.0
49	34.51622936573216	36.0	36.0	36.0	32.0	36.0
50	34.11639978940202	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	5.0
20	2.0
21	18.0
22	63.0
23	179.0
24	423.0
25	1029.0
26	2232.0
27	4285.0
28	7620.0
29	12317.0
30	18179.0
31	27129.0
32	40365.0
33	67686.0
34	152671.0
35	611674.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.06091531607752	17.623668138434287	12.280781535044815	26.03463501044338
2	15.804141979302791	19.778331789712293	38.090857957260724	26.326668273724184
3	19.289270335446727	23.107964811343596	28.115508107325194	29.487256745884487
4	12.482265154702826	15.74611102066017	35.72944925244059	36.042174572196416
5	14.434102495247803	36.920934835147875	33.271309830654694	15.373652838949631
6	34.996410738394104	35.12909183752221	16.3618525453098	13.512644878773877
7	30.461222271794036	30.286992614269494	19.767559875586493	19.48422523834998
8	27.48398842133975	34.09636337878669	18.88531079060936	19.534337409264197
9	26.761802262025334	14.852126807051228	18.355855617743515	40.03021531317992
10	15.72096583382406	27.420584283157325	31.608020916038765	25.25042896697985
11	37.21145856019486	21.567580597074887	21.859055819037973	19.361905023692273
12	24.52240140906121	23.601986725560803	29.038100050957944	22.83751181442004
13	29.612909910157548	19.940097983037983	25.09900854021343	25.347983566591044
14	23.190517170290462	19.535183184300724	25.656162845525532	31.618136799883285
15	24.82550603777654	27.532726207819614	22.52647804473727	25.115289709666573
16	25.101150370717296	26.328698794441934	24.380754628812234	24.18939620602853
17	23.72853581540114	26.39156423978568	25.48838222265451	24.39151772215867
18	24.024582477425714	26.041758072138343	26.53389394181273	23.39976550862321
19	25.153878195708113	25.224183245619415	25.777531563267143	23.844406995405325
20	25.163340649302867	25.120206031234538	25.734240006089593	23.982213313373
21	25.687879409395293	24.703080101239273	25.42907224821806	24.179968241147378
22	25.020245739936865	25.00343596108589	25.84815377881714	24.128164520160105
23	24.29499364611504	25.00364740484502	25.971002602872673	24.730356346167266
24	24.442494436309623	25.14682327816337	26.022106748848937	24.38857553667807
25	24.6202185705692	24.830923390349444	26.19749162942594	24.35136640965542
26	23.95593916314435	25.835787853350446	26.23192941131694	23.97634357218826
27	24.28285826955627	25.296842176486695	26.16133455548472	24.258964998472308
28	24.22349019471025	25.592075841529514	25.829424221375735	24.3550097423845
29	24.20546944462946	25.230342762717616	25.87472102491561	24.68946676773732
30	24.17945478477373	25.59805046174666	25.89333572972887	24.329159023750748
31	24.603699858543525	25.261821341954636	25.712409898147108	24.422068901354727
32	24.380122912906913	25.518224962547826	25.56072538279452	24.54092674175074
33	24.114048775949488	25.13246979519514	26.065995965644547	24.687485463210823
34	24.622412710361	25.3576057699017	26.008855302080402	24.011126217656898
35	24.648976558262863	25.29842675104691	26.18374270650412	23.868853984186106
36	24.10638581296954	25.647916538919397	25.797724442264226	24.447973205846843
37	24.53212188618684	25.479824837767715	25.655113937915097	24.33293933813035
38	24.143705636456286	25.522636111633844	25.8188688181774	24.514789433732467
39	24.8326404952911	25.187552597191154	25.621334055068996	24.358472852448756
40	25.349146507266262	25.237187036806013	25.512381089315962	23.901285366611763
41	24.165371562688318	25.549599839301386	26.226648482349585	24.058380115660714
42	24.958898750839207	25.843936838877816	25.556360253109684	23.640804157173292
43	24.391097446611003	25.320534846766	25.936811789719417	24.351555916903582
44	24.010297724773746	25.66966928867462	25.69842679522964	24.621606191321998
45	24.24015917856418	25.91758153460992	25.617007240002703	24.225252046823194
46	24.283166342623282	25.49719250502981	25.382270756485358	24.837370395861548
47	24.444082637236235	25.585149740608898	25.706413890295593	24.264353731859277
48	24.64026761340689	25.588523241725365	25.258658422865555	24.512550722002192
49	24.304247938620094	25.774676031112854	25.35558076517661	24.56549526509044
50	24.62904404107554	25.93091840607612	25.156602355299423	24.283435197548915
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	178.0
1	117.0
2	56.0
3	137.0
4	218.0
5	224.5
6	231.0
7	178.5
8	126.0
9	203.5
10	281.0
11	442.5
12	604.0
13	1137.0
14	1670.0
15	2287.5
16	2905.0
17	2942.0
18	2979.0
19	2910.0
20	2841.0
21	3073.0
22	3305.0
23	3735.0
24	4165.0
25	5127.5
26	6090.0
27	8133.5
28	10177.0
29	12233.0
30	14289.0
31	16490.0
32	18691.0
33	21599.0
34	24507.0
35	28063.5
36	31620.0
37	35286.5
38	38953.0
39	41650.0
40	44347.0
41	46972.0
42	49597.0
43	51874.0
44	54151.0
45	57956.5
46	61762.0
47	64446.5
48	67131.0
49	69095.0
50	71059.0
51	70350.0
52	69641.0
53	68244.5
54	66848.0
55	66158.0
56	65468.0
57	62009.5
58	58551.0
59	54056.5
60	49562.0
61	43724.0
62	37886.0
63	32470.0
64	27054.0
65	22883.5
66	18713.0
67	16088.5
68	13464.0
69	11847.5
70	10231.0
71	8068.0
72	5905.0
73	5157.0
74	4409.0
75	3365.0
76	2321.0
77	1932.5
78	1544.0
79	1314.0
80	1084.0
81	841.0
82	598.0
83	475.5
84	353.0
85	263.0
86	173.0
87	123.5
88	74.0
89	47.0
90	20.0
91	16.5
92	13.0
93	10.5
94	8.0
95	8.0
96	8.0
97	8.5
98	9.0
99	8.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03203372950845669
2	0.005286093978293184
3	5.286093978293184E-4
4	0.0
5	0.0
6	1.0572187956586368E-4
7	0.0
8	0.0
9	0.0
10	1.0572187956586368E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	9.514969160927731E-4
17	0.0
18	1.0572187956586368E-4
19	0.0
20	2.1144375913172736E-4
21	0.0
22	0.0
23	0.0
24	0.0013743844343562278
25	5.286093978293184E-4
26	4.228875182634547E-4
27	5.286093978293184E-4
28	0.0011629406752245003
29	5.286093978293184E-4
30	0.0013743844343562278
31	4.228875182634547E-4
32	5.286093978293184E-4
33	2.1144375913172736E-4
34	4.228875182634547E-4
35	1.0572187956586368E-4
36	0.0
37	0.001268662554790364
38	0.0
39	0.0010572187956586368
40	0.0
41	8.457750365269094E-4
42	0.004546040821332138
43	0.003911709543936956
44	0.00401743142350282
45	0.0023258813504490007
46	0.0017972719526196824
47	9.514969160927731E-4
48	0.0038059876643710923
49	0.003277378266541774
50	0.0011629406752245003
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	945878.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.70681716927639
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.00648387761552	38.99838398034223
2	16.536690408372003	18.424127783282117
3	6.256407618034662	10.45573665942975
4	2.8526855329756398	6.356561257076549
5	1.4565562560222953	4.057005652549988
6	0.8626210150560145	2.8832322703260607
7	0.534311245744611	2.0835345214728345
8	0.31979532148438994	1.42518236044167
9	0.24154329540553393	1.211004737605854
>10	0.8412454758128001	7.83300913316841
>50	0.055954900884996155	2.211227880302528
>100	0.03398662774540365	3.4792867466265776
>500	0.0015274887521285045	0.4635361458624632
>1k	1.9093609401606306E-4	0.11817087151289507
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1111	0.11745700819767455	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.286093978293184E-4
2	0.0	0.0	0.0	0.0	5.286093978293184E-4
3	0.0	0.0	0.0	0.0	5.286093978293184E-4
4	0.0	0.0	0.0	0.0	5.286093978293184E-4
5	0.0	0.0	0.0	0.0	6.34331277395182E-4
6	0.0	0.0	0.0	0.0	7.400531569610457E-4
7	0.0	0.0	0.0	0.0	7.400531569610457E-4
8	0.0	0.0	0.0	0.0	7.400531569610457E-4
9	0.0	0.0	0.0	0.0	7.400531569610457E-4
10	0.0	0.0	0.0	0.0	7.400531569610457E-4
11	0.0	0.0	0.0	0.0	7.400531569610457E-4
12	0.0	0.0	0.0	0.0	0.0013743844343562278
13	0.0	0.0	0.0	0.0	0.0014801063139220914
14	0.0	0.0	0.0	0.0	0.0014801063139220914
15	0.0	0.0	0.0	0.0	0.0016915500730538189
16	0.0	0.0	0.0	1.0572187956586368E-4	0.00200871571175141
17	0.0	0.0	0.0	1.0572187956586368E-4	0.0021144375913172736
18	0.0	0.0	0.0	1.0572187956586368E-4	0.0022201594708831374
19	0.0	0.0	0.0	1.0572187956586368E-4	0.0023258813504490007
20	0.0	0.0	0.0	1.0572187956586368E-4	0.0029602126278441827
21	0.0	0.0	0.0	1.0572187956586368E-4	0.00317165638697591
22	0.0	0.0	0.0	3.17165638697591E-4	0.00317165638697591
23	0.0	0.0	0.0	7.400531569610457E-4	0.00317165638697591
24	0.0	0.0	0.0	9.514969160927731E-4	0.00317165638697591
25	0.0	0.0	0.0	0.0010572187956586368	0.00317165638697591
26	0.0	0.0	0.0	0.0011629406752245003	0.003277378266541774
27	0.0	0.0	0.0	0.0014801063139220914	0.003277378266541774
28	0.0	0.0	0.0	0.00200871571175141	0.003277378266541774
29	0.0	0.0	0.0	0.002537325109580728	0.0033831001461076377
30	0.0	0.0	0.0	0.005074650219161456	0.0033831001461076377
31	0.0	0.0	0.0	0.011312241113547413	0.0033831001461076377
32	0.0	0.0	0.0	0.018184163285328552	0.0034888220256735014
33	0.0	0.0	0.0	0.024844641697977964	0.0035945439052393648
34	0.0	0.0	0.0	0.031187954471929784	0.0038059876643710923
35	0.0	0.0	0.0	0.039857148596330605	0.0038059876643710923
36	0.0	0.0	0.0	0.051909442866839066	0.0038059876643710923
37	0.0	0.0	0.0	0.06882494359737726	0.0038059876643710923
38	0.0	0.0	0.0	0.09229520086099899	0.00401743142350282
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1630	0.0	24.166302	1
GATATAC	365	0.0	23.51346	1
TAGGACG	230	0.0	21.04272	4
GTCCTAC	325	0.0	20.313402	1
CGATTAA	55	0.0044838376	19.99928	27
AACGACT	55	0.0044838376	19.99928	18
GTAGGAC	395	0.0	19.49297	3
AATCCCG	295	0.0	19.389132	19
GTCCTAA	140	4.0636223E-9	18.862448	1
GTACTAG	70	8.1061956E-4	18.862448	1
GGAATAT	490	0.0	18.856462	12
TACACCG	70	8.123505E-4	18.856462	5
AACGCAG	2130	0.0	18.69416	6
CAACGTA	95	1.5970068E-5	18.525648	20
TGTAGGA	505	0.0	18.296371	2
ATCAACG	2235	0.0	17.914345	3
ATACACT	505	0.0	17.860743	4
GGACGTG	285	0.0	17.753746	6
ATCCCGT	315	0.0	17.459688	20
TCCCGTT	290	0.0	17.447647	21
>>END_MODULE