FastQCFastQC Report
Thu 2 Feb 2017
SRR4062223_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062223_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1360392
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT98240.7221447935595035No Hit
CTGTAGGACGTGGAATATGGCAAGA97230.714720462925392No Hit
GTCCTACAGTGGACATTTCTAAATT93990.6909037983169557No Hit
CTTTAGGACGTGAAATATGGCGAGG84840.6236437732653529No Hit
GTCCTACAGTGTGCATTTCTCATTT58360.4289939958482555No Hit
CTGTAGGACCTGGAATATGGCGAGA42790.3145416909243806No Hit
GATATACACTGTTCTACAAATCCCG41230.3030744079647631No Hit
ATTTAGAAATGTCCACTGTAGGACG38750.28484436838793525No Hit
CTGAAGGACCTGGAATATGGCGAGA38110.28013984204552805No Hit
TTGTAGAACAGTGTATATCAATGAG38100.280066333821428No Hit
GTGTATATCAATGAGTTACAATGAA36390.2674964275003087No Hit
GTCCTTCAGTGTGCATTTCTCATTT34320.2522802251115855No Hit
GTGTATATCAATGAGTTACAATGAG31610.23235949638045506No Hit
TTTCTAAATTTTCCACCTTTTTCAG28060.20626407682491518No Hit
GAATATGGCAAGAAAACTGAAAATC27840.20464689589471272No Hit
GCGCAAGACGGACCAGAGCGAAAGC27680.20347076430911093No Hit
GTTCTACAGTGTGGTTTTTATCATT25230.1854612494045834No Hit
GGAATATGGCGAGAAAACTGAAAAT24320.17877200101147317No Hit
GATATACACTGTTCTACAATGCCGG23440.17230327729066328No Hit
GAATAGGACCGCGGTTCTATTTTGT23370.1717887197219625No Hit
GATTAAGAGGGACGGCCGGGGGCAT22780.16745173450005588No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG21900.16098301077924598No Hit
GGGTAGGCACACGCTGAGCCAGTCA21530.15826320648754183No Hit
TCGTAGTTCCGACCATAAACGATGC21450.15767514069474092No Hit
ATTCCAGGTCCTTCAGTGTGCATTT21440.15760163247064082No Hit
GTATCAACGCAGAGTACTTTTTTTT20790.15282359790413352No Hit
GAACTACGACGGTATCTGATCGTCT20330.14944221959552834No Hit
ATCAGATACCGTCGTAGTTCCGACC20240.14878064557862736No Hit
CTGTAGGACATGGAATATGGCAAGA19840.14584031661462285No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC19550.14370857811571958No Hit
GACCTGGAATATGGCGAGAAAACTG18810.13826896953231127No Hit
TTCCAGGTCCTTCAGTGTGCATTTC18650.13709283794670946No Hit
GTATCTGATCGTCTTCGAACCTCCG18570.13650477215390858No Hit
GAATAACGCCGCCGCATCGCCAGTC18530.13621073925750815No Hit
GTTCTACAAATCCCGTTTCCAACGA18500.1359902145852078No Hit
ACCTGGAATATGGCGAGAAAACTGA18480.13584319813700757No Hit
GTCTTGCGCCGGTCCAAGAATTTCA18430.135475657016507No Hit
CTGTAGAACATATTAGATGAGTGAG18080.1329028691730031No Hit
GAAATATGGCGAGGAAAACTGAAAA18070.13282936094890296No Hit
ACCATACTCCCCCCGGAACCCAAAG17580.12922745796799745No Hit
CCATATTCCAGGTCCTTCAGTGTGC17390.1278308017100953No Hit
CCATATTTCACGTCCTAAAGTGTGT17390.1278308017100953No Hit
GTCCACTGTAGGACGTGGAATATGG17070.12547853853889174No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT16900.12422889872918982No Hit
GCCATATTCCACGTCCTACAGTGGA16280.11967138883498286No Hit
ACAGTGGACATTTCTAAATTTTCCA15730.11562843650947667No Hit
GTGCATGGCCGTTCTTAGTTGGTGG15550.11430528847567467No Hit
GCCATGCACCACCACCCACGGAATC15320.11261459932137208No Hit
GTCCTATTCCATTATTCCTAGCTGC15320.11261459932137208No Hit
GTCCTACAGTGGACATTTCTAAATA15060.11070338549476916No Hit
ATACACACTTTAGGACGTGAAATAT15040.11055636904656893No Hit
GAATAATGGAATAGGACCGCGGTTC15000.11026233615016848No Hit
CCACTGTAGGACGTGGAATATGGCA15000.11026233615016848No Hit
CCTAAAGTGTGTATTTCTCATTTTC14720.10820410587536533No Hit
CATCTAAGGGCATCACAGACCTGTT14660.10776305653076466No Hit
ATGTTCTACAGTGTGGTTTTTATCA14470.10636640027286252No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG14090.10357308775705826No Hit
GAAATACACACTTTAGGACGTGAAA13900.10217643149915613No Hit
GTAGAACATATTAGATGAGTGAGTT13800.101441349258155No Hit
GCTTTGAACACTCTAATTTTTTCAA13770.10122082458585467No Hit
GAGTATGGTTGCAAAGCTGAAACTT13770.10122082458585467No Hit
GTGTATTTCTCATTTTCCGTGATTT13740.10100029991355433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGT2400.016.62261819
CACCTCG350.002166754316.28937510
CGGTCCA3200.014.84708710
AGGCCCG3300.014.6851210
GCGTTAT2350.014.5462881
CGGTTCT3400.014.53214312
GGTCCCG400.005272073714.25267812
CGAATAT400.005272073714.25267812
TGCGTTT400.005272073714.25267812
TCCAACG6350.014.06149318
CCAGGAC1101.8735591E-1013.8151843
GTAAGAC903.6478013E-813.7192453
GGGTAGG4100.013.4326481
CGTTATT2550.013.4083622
AAATGTC10850.013.3978457
TAGAAAT11150.013.2048714
TGGCGAC655.4569555E-513.15244518
AATGTCC11150.013.1225778
CGCCGGT3700.013.0960917
ATTTAGA10750.013.0728641
Produced by FastQC (version 0.11.5)