##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062223_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1360392
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.404197466612565	32.0	32.0	32.0	32.0	32.0
2	31.07389561244112	32.0	32.0	32.0	32.0	32.0
3	31.07605528406518	32.0	32.0	32.0	32.0	32.0
4	31.08177422390017	32.0	32.0	32.0	32.0	32.0
5	31.08751668636687	32.0	32.0	32.0	32.0	32.0
6	34.772825038665324	36.0	36.0	36.0	36.0	36.0
7	34.68804212315274	36.0	36.0	36.0	32.0	36.0
8	34.69007977112479	36.0	36.0	36.0	32.0	36.0
9	34.72155231727326	36.0	36.0	36.0	32.0	36.0
10	34.50189357185282	36.0	36.0	36.0	32.0	36.0
11	34.67530241283395	36.0	36.0	36.0	32.0	36.0
12	34.54181515327935	36.0	36.0	36.0	32.0	36.0
13	34.59628915783098	36.0	36.0	36.0	32.0	36.0
14	34.5738074025722	36.0	36.0	36.0	32.0	36.0
15	34.52630932848767	36.0	36.0	36.0	32.0	36.0
16	34.52589841751495	36.0	36.0	36.0	32.0	36.0
17	34.4710708384054	36.0	36.0	36.0	32.0	36.0
18	34.48265058894789	36.0	36.0	36.0	32.0	36.0
19	34.37906353462825	36.0	36.0	36.0	32.0	36.0
20	34.35099147892666	36.0	36.0	36.0	32.0	36.0
21	34.299361507565465	36.0	36.0	36.0	32.0	36.0
22	34.22960587830566	36.0	36.0	36.0	32.0	36.0
23	34.23310413469059	36.0	36.0	36.0	32.0	36.0
24	34.16529647336944	36.0	36.0	36.0	32.0	36.0
25	33.82330828173056	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	24.0
5	108.0
6	294.0
7	40.0
8	256.0
9	196.0
10	92.0
11	28.0
12	71.0
13	49.0
14	221.0
15	273.0
16	422.0
17	549.0
18	701.0
19	1010.0
20	1466.0
21	1946.0
22	2899.0
23	4355.0
24	5964.0
25	8685.0
26	12057.0
27	15315.0
28	20760.0
29	28084.0
30	38173.0
31	53295.0
32	77439.0
33	116313.0
34	279711.0
35	689592.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.787298824297366	19.744953240937445	12.095535211536793	25.372212723228397
2	12.415566429896154	20.820662325261377	46.528235673766844	20.235535571075626
3	19.481541922109997	25.962304650171276	29.65641952983725	24.899733897881475
4	10.430817163874611	17.086755244888913	40.084206409361954	32.39822118187452
5	11.236535063433832	40.333078171452605	36.15224707905827	12.278139686055294
6	30.4803657267274	39.76549943544798	18.334810608428928	11.41932422939569
7	26.78429242776804	31.520083362401262	22.597554173050572	19.098070036780125
8	22.6903000808762	42.65656609513661	20.27129954587167	14.381834278115518
9	26.68387890688536	14.04693966319846	20.651449927615857	38.61773150230032
10	15.719343702384196	28.568231838825792	33.72593906649099	21.98648539229903
11	36.03533769717528	20.204558771999565	27.306402362717126	16.453701168108033
12	25.414420356210123	23.272978615026307	33.83929912169384	17.473301907069732
13	30.598737516487656	22.02751083474656	26.277131029772	21.096620618993782
14	19.295420557446157	25.565585883682868	26.17606537687081	28.962928182000162
15	20.707719803434884	38.233696372260646	21.68134427455518	19.37723954974929
16	19.676783556264066	27.300366963564183	33.68476755294329	19.338081927228462
17	18.977735251904466	30.942479667316825	30.149560225223563	19.930224855555146
18	19.7931002000262	26.497878309300262	36.28164689709175	17.427374593581792
19	24.749794883681574	24.89409375379419	26.273358425589688	24.08275293693455
20	24.21027537813265	28.27204213336198	28.164545552871616	19.353136935633756
21	23.13982149820836	26.461087933838083	25.140498421738073	25.25859214621548
22	23.47438638061143	31.546604167203235	26.87128618155996	18.107723270625375
23	19.66914131385594	30.58073895108409	29.401927973567798	20.348191761492174
24	24.057602274424568	27.103853763447187	30.243769281877224	18.594774680251017
25	21.488930107855207	28.983759547418693	30.001096283891908	19.526214060834192
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	885.0
1	885.0
2	731.5
3	578.0
4	578.0
5	578.0
6	1109.0
7	1640.0
8	1640.0
9	1640.0
10	2057.0
11	2474.0
12	2474.0
13	2474.0
14	3490.5
15	4507.0
16	4507.0
17	4507.0
18	8686.5
19	12866.0
20	12866.0
21	12866.0
22	25955.5
23	39045.0
24	39045.0
25	39045.0
26	65800.0
27	92555.0
28	92555.0
29	92555.0
30	127610.0
31	162665.0
32	162665.0
33	162665.0
34	170496.5
35	178328.0
36	178328.0
37	178328.0
38	174936.0
39	171544.0
40	171544.0
41	171544.0
42	168232.5
43	164921.0
44	164921.0
45	164921.0
46	161714.5
47	158508.0
48	158508.0
49	158508.0
50	138368.0
51	118228.0
52	118228.0
53	118228.0
54	90647.0
55	63066.0
56	63066.0
57	63066.0
58	58573.0
59	54080.0
60	54080.0
61	54080.0
62	51404.0
63	48728.0
64	48728.0
65	48728.0
66	42112.0
67	35496.0
68	35496.0
69	35496.0
70	27316.5
71	19137.0
72	19137.0
73	19137.0
74	16047.5
75	12958.0
76	12958.0
77	12958.0
78	11676.0
79	10394.0
80	10394.0
81	10394.0
82	7265.5
83	4137.0
84	4137.0
85	4137.0
86	3182.5
87	2228.0
88	2228.0
89	2228.0
90	1488.0
91	748.0
92	748.0
93	748.0
94	441.0
95	134.0
96	134.0
97	134.0
98	338.0
99	542.0
100	542.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010291151374015725
2	2.2052467230033697E-4
3	8.820986892013479E-4
4	0.011981840528318308
5	0.0346223735511529
6	0.06667195925880187
7	0.11070338549476916
8	0.11210004175267128
9	0.12268522602308747
10	0.13687231327440913
11	0.1437820863398197
12	0.1373133626190098
13	0.13231480338020218
14	0.11937735593858241
15	0.13672529682620893
16	0.12305276714358804
17	0.12805132638239566
18	0.11540791183717634
19	0.10342607130885803
20	0.09401701862404366
21	0.09570770777834624
22	0.10636640027286252
23	0.08387288369822817
24	0.09401701862404366
25	0.09225282124564096
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1360392.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.621810567197684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.29343913027543	22.544276268616084
2	15.185008272234676	9.603549100918693
3	5.572480295066546	5.286357488401085
4	2.49490294231786	3.15573392902078
5	1.3208255747666657	2.0883448058790752
6	0.7919074065931034	1.5024927598828746
7	0.5321116042769433	1.177843264573721
8	0.36624901792283043	0.9265165652142346
9	0.26822638417735745	0.7633623518622706
>10	1.6776565071912461	10.316542466249077
>50	0.2133865032612612	4.713600799277554
>100	0.2262055637965061	15.376874852426878
>500	0.0349802417730031	7.4490321608742525
>1k	0.021454548287441904	11.904927069706956
>5k	0.0011660080591001036	3.1905461170964955
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	9824	0.7221447935595035	No Hit
CTGTAGGACGTGGAATATGGCAAGA	9723	0.714720462925392	No Hit
GTCCTACAGTGGACATTTCTAAATT	9399	0.6909037983169557	No Hit
CTTTAGGACGTGAAATATGGCGAGG	8484	0.6236437732653529	No Hit
GTCCTACAGTGTGCATTTCTCATTT	5836	0.4289939958482555	No Hit
CTGTAGGACCTGGAATATGGCGAGA	4279	0.3145416909243806	No Hit
GATATACACTGTTCTACAAATCCCG	4123	0.3030744079647631	No Hit
ATTTAGAAATGTCCACTGTAGGACG	3875	0.28484436838793525	No Hit
CTGAAGGACCTGGAATATGGCGAGA	3811	0.28013984204552805	No Hit
TTGTAGAACAGTGTATATCAATGAG	3810	0.280066333821428	No Hit
GTGTATATCAATGAGTTACAATGAA	3639	0.2674964275003087	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	3432	0.2522802251115855	No Hit
GTGTATATCAATGAGTTACAATGAG	3161	0.23235949638045506	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	2806	0.20626407682491518	No Hit
GAATATGGCAAGAAAACTGAAAATC	2784	0.20464689589471272	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2768	0.20347076430911093	No Hit
GTTCTACAGTGTGGTTTTTATCATT	2523	0.1854612494045834	No Hit
GGAATATGGCGAGAAAACTGAAAAT	2432	0.17877200101147317	No Hit
GATATACACTGTTCTACAATGCCGG	2344	0.17230327729066328	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2337	0.1717887197219625	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2278	0.16745173450005588	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2190	0.16098301077924598	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2153	0.15826320648754183	No Hit
TCGTAGTTCCGACCATAAACGATGC	2145	0.15767514069474092	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	2144	0.15760163247064082	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2079	0.15282359790413352	No Hit
GAACTACGACGGTATCTGATCGTCT	2033	0.14944221959552834	No Hit
ATCAGATACCGTCGTAGTTCCGACC	2024	0.14878064557862736	No Hit
CTGTAGGACATGGAATATGGCAAGA	1984	0.14584031661462285	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1955	0.14370857811571958	No Hit
GACCTGGAATATGGCGAGAAAACTG	1881	0.13826896953231127	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	1865	0.13709283794670946	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1857	0.13650477215390858	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1853	0.13621073925750815	No Hit
GTTCTACAAATCCCGTTTCCAACGA	1850	0.1359902145852078	No Hit
ACCTGGAATATGGCGAGAAAACTGA	1848	0.13584319813700757	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1843	0.135475657016507	No Hit
CTGTAGAACATATTAGATGAGTGAG	1808	0.1329028691730031	No Hit
GAAATATGGCGAGGAAAACTGAAAA	1807	0.13282936094890296	No Hit
ACCATACTCCCCCCGGAACCCAAAG	1758	0.12922745796799745	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	1739	0.1278308017100953	No Hit
CCATATTTCACGTCCTAAAGTGTGT	1739	0.1278308017100953	No Hit
GTCCACTGTAGGACGTGGAATATGG	1707	0.12547853853889174	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	1690	0.12422889872918982	No Hit
GCCATATTCCACGTCCTACAGTGGA	1628	0.11967138883498286	No Hit
ACAGTGGACATTTCTAAATTTTCCA	1573	0.11562843650947667	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	1555	0.11430528847567467	No Hit
GCCATGCACCACCACCCACGGAATC	1532	0.11261459932137208	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1532	0.11261459932137208	No Hit
GTCCTACAGTGGACATTTCTAAATA	1506	0.11070338549476916	No Hit
ATACACACTTTAGGACGTGAAATAT	1504	0.11055636904656893	No Hit
GAATAATGGAATAGGACCGCGGTTC	1500	0.11026233615016848	No Hit
CCACTGTAGGACGTGGAATATGGCA	1500	0.11026233615016848	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	1472	0.10820410587536533	No Hit
CATCTAAGGGCATCACAGACCTGTT	1466	0.10776305653076466	No Hit
ATGTTCTACAGTGTGGTTTTTATCA	1447	0.10636640027286252	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	1409	0.10357308775705826	No Hit
GAAATACACACTTTAGGACGTGAAA	1390	0.10217643149915613	No Hit
GTAGAACATATTAGATGAGTGAGTT	1380	0.101441349258155	No Hit
GCTTTGAACACTCTAATTTTTTCAA	1377	0.10122082458585467	No Hit
GAGTATGGTTGCAAAGCTGAAACTT	1377	0.10122082458585467	No Hit
GTGTATTTCTCATTTTCCGTGATTT	1374	0.10100029991355433	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	7.350822410011232E-5	0.0
8	0.0	0.0	0.0	7.350822410011232E-5	0.0
9	0.0	0.0	0.0	2.9403289640044926E-4	0.0
10	0.0	0.0	0.0	3.675411205005616E-4	0.0
11	0.0	0.0	0.0	3.675411205005616E-4	0.0
12	0.0	0.0	0.0	3.675411205005616E-4	0.0
13	0.0	0.0	0.0	3.675411205005616E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAACGT	240	0.0	16.622618	19
CACCTCG	35	0.0021667543	16.289375	10
CGGTCCA	320	0.0	14.847087	10
AGGCCCG	330	0.0	14.68512	10
GCGTTAT	235	0.0	14.546288	1
CGGTTCT	340	0.0	14.532143	12
GGTCCCG	40	0.0052720737	14.252678	12
CGAATAT	40	0.0052720737	14.252678	12
TGCGTTT	40	0.0052720737	14.252678	12
TCCAACG	635	0.0	14.061493	18
CCAGGAC	110	1.8735591E-10	13.815184	3
GTAAGAC	90	3.6478013E-8	13.719245	3
GGGTAGG	410	0.0	13.432648	1
CGTTATT	255	0.0	13.408362	2
AAATGTC	1085	0.0	13.397845	7
TAGAAAT	1115	0.0	13.204871	4
TGGCGAC	65	5.4569555E-5	13.152445	18
AATGTCC	1115	0.0	13.122577	8
CGCCGGT	370	0.0	13.096091	7
ATTTAGA	1075	0.0	13.072864	1
>>END_MODULE