##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062223_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1360392
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24389955248193	32.0	32.0	32.0	32.0	32.0
2	31.53579335956107	32.0	32.0	32.0	32.0	32.0
3	31.6466915418497	32.0	32.0	32.0	32.0	32.0
4	31.717349852101453	32.0	32.0	32.0	32.0	32.0
5	31.711038435980218	32.0	32.0	32.0	32.0	32.0
6	35.38555725114526	36.0	36.0	36.0	36.0	36.0
7	35.38230745255779	36.0	36.0	36.0	36.0	36.0
8	35.34485133696758	36.0	36.0	36.0	36.0	36.0
9	35.40543828543537	36.0	36.0	36.0	36.0	36.0
10	35.312204129398	36.0	36.0	36.0	36.0	36.0
11	35.39959291145493	36.0	36.0	36.0	36.0	36.0
12	35.32651985604149	36.0	36.0	36.0	36.0	36.0
13	35.35784906115296	36.0	36.0	36.0	36.0	36.0
14	35.332437268081556	36.0	36.0	36.0	36.0	36.0
15	35.303134684708525	36.0	36.0	36.0	36.0	36.0
16	35.31232394780328	36.0	36.0	36.0	36.0	36.0
17	35.31205270245635	36.0	36.0	36.0	36.0	36.0
18	35.30374039247511	36.0	36.0	36.0	36.0	36.0
19	35.25500443989674	36.0	36.0	36.0	36.0	36.0
20	35.228576028086025	36.0	36.0	36.0	36.0	36.0
21	35.19028559415227	36.0	36.0	36.0	36.0	36.0
22	35.13780292739152	36.0	36.0	36.0	36.0	36.0
23	35.14026986339232	36.0	36.0	36.0	36.0	36.0
24	35.11431999012049	36.0	36.0	36.0	36.0	36.0
25	35.03135272774318	36.0	36.0	36.0	36.0	36.0
26	35.033860093267236	36.0	36.0	36.0	36.0	36.0
27	34.966281042523036	36.0	36.0	36.0	36.0	36.0
28	34.88473028362413	36.0	36.0	36.0	36.0	36.0
29	34.82528785820558	36.0	36.0	36.0	32.0	36.0
30	34.738753241712686	36.0	36.0	36.0	32.0	36.0
31	34.74842692400426	36.0	36.0	36.0	32.0	36.0
32	34.694698292845004	36.0	36.0	36.0	32.0	36.0
33	34.75478979588237	36.0	36.0	36.0	32.0	36.0
34	34.72367523478527	36.0	36.0	36.0	32.0	36.0
35	34.70480273332981	36.0	36.0	36.0	32.0	36.0
36	34.60653326394157	36.0	36.0	36.0	32.0	36.0
37	34.46818123011602	36.0	36.0	36.0	32.0	36.0
38	34.4200090856165	36.0	36.0	36.0	32.0	36.0
39	34.17209818934543	36.0	36.0	36.0	32.0	36.0
40	34.313293521279164	36.0	36.0	36.0	32.0	36.0
41	34.29618301195538	36.0	36.0	36.0	32.0	36.0
42	34.25578877264788	36.0	36.0	36.0	32.0	36.0
43	34.32579800528083	36.0	36.0	36.0	32.0	36.0
44	34.13091006121765	36.0	36.0	36.0	32.0	36.0
45	34.11557698075261	36.0	36.0	36.0	32.0	36.0
46	33.89329546189628	36.0	36.0	36.0	32.0	36.0
47	33.93869781651171	36.0	36.0	36.0	32.0	36.0
48	33.863415838964066	36.0	36.0	36.0	32.0	36.0
49	33.81022455292298	36.0	36.0	36.0	32.0	36.0
50	33.535974189792356	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	12.0
21	26.0
22	88.0
23	238.0
24	684.0
25	1827.0
26	4233.0
27	8506.0
28	14378.0
29	22406.0
30	31825.0
31	45069.0
32	65855.0
33	110222.0
34	241778.0
35	813244.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.392421910559754	20.26674568935407	12.442176055390394	25.898656344695787
2	12.027628833846203	21.24032762927492	46.16501435693713	20.56702917994175
3	19.111662589386526	26.288900780955966	29.950881515807303	24.648555113850207
4	10.279904615728409	17.457762174432077	40.42195190797946	31.840381301860056
5	10.955004145863839	40.61064751924445	36.024395909414345	12.409952425477362
6	30.67789383926668	39.9112019347393	18.097824888451104	11.313079337542911
7	26.749348717134474	31.489085498885615	22.82048115543167	18.94108462854824
8	22.678169233573854	42.734961687513604	20.18153591023764	14.405333168674911
9	26.47244323694935	13.825794329869625	20.708883909931846	38.99287852324918
10	15.766423035730167	28.75327755035133	33.44435533607617	22.035944077842327
11	36.234702938564766	20.01908273497639	27.44826491187834	16.297949414580504
12	25.447591576545587	23.20831054578386	33.79592058759534	17.548177290075216
13	30.730186593276056	21.789381295979393	26.39121664931873	21.089215461425827
14	19.356626619386176	25.46905597798282	26.249198760357306	28.925118642273695
15	20.76497068492023	38.13378790819117	21.68036859963893	19.420872807249676
16	19.729753371650858	27.353025278836096	33.450040319409105	19.467181030103934
17	19.075898711547847	31.06656022675817	29.984813200900916	19.872727860793066
18	19.973375301659598	26.335590282499666	36.379981931665235	17.311052484175505
19	25.049691559491677	24.631503272586137	26.45979982240413	23.859005345518057
20	24.561780077771818	28.168686920662456	28.123626313042582	19.145906688523144
21	23.287405394915584	26.326749936782928	25.30902857411687	25.07681609418462
22	23.690083446535997	31.207916541702684	27.059700439285145	18.04229957247617
23	19.669551129380356	30.398811519032748	29.51994719169181	20.411690159895088
24	24.34437629375235	26.81691345972902	30.249694203989463	18.58901604252917
25	21.647705178445285	28.912980037327557	29.891817708023222	19.54749707620394
26	17.405254971375815	32.1667788895521	31.80372070321114	18.624245435860946
27	19.78112126834403	29.480706974774844	30.263792388642063	20.474379368239063
28	18.466893319856656	31.226022236515664	27.79865845814573	22.508425985481946
29	20.68248427097369	27.090900136211644	27.471307712607405	24.755307880207265
30	18.375774968427894	29.069861464975176	30.127214641812788	22.42714892478414
31	23.688735174233766	26.601072490508205	28.09263554067415	21.617556794583887
32	20.84973191353605	30.045972381287022	28.21508958513124	20.889206120045692
33	18.904018559410993	30.051500013231518	31.571654557376277	19.472826869981212
34	20.92651780673692	30.201031473466315	30.946776792435077	17.925673927361686
35	20.11235030006145	30.608620481803722	30.805476084764056	18.473553133370775
36	17.548375904521357	30.47123320699683	31.406187058398043	20.57420383008377
37	19.522188816277925	29.89591067136147	27.79942221601476	22.782478296345847
38	20.533052237884377	31.380954901234347	27.496045257543418	20.589947603337862
39	23.985468787443526	26.3807518770463	28.407241495403497	21.226537840106676
40	25.525088007785996	27.17064432211033	29.458368954219775	17.84589871588389
41	22.962966774553745	26.135332000855648	32.335984321946995	18.565716902643615
42	22.361536052599465	31.632383016324123	29.48869308499924	16.517387846077167
43	19.06657090622264	30.845252543667424	31.11717005834575	18.971006491764186
44	18.0155961239045	31.44865467258872	28.74792882357179	21.787820379934985
45	18.644954894557944	31.85718864307305	28.82528845136022	20.672568011008785
46	19.470979437106127	27.654603954973783	29.156114948730593	23.718301659189496
47	23.20799016739539	27.423234232989213	29.12923817896869	20.239537420646705
48	20.446315548047618	29.073730205287212	27.21463342198777	23.265320824677396
49	22.260668719106807	28.921910239559168	27.575033153671306	21.242387887662716
50	20.112176188655944	32.62592254991326	26.020318151077653	21.24158311035314
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	336.0
1	183.0
2	30.0
3	132.5
4	235.0
5	231.5
6	228.0
7	177.0
8	126.0
9	204.0
10	282.0
11	459.5
12	637.0
13	1105.5
14	1574.0
15	2121.0
16	2668.0
17	2885.0
18	3102.0
19	3442.0
20	3782.0
21	4677.5
22	5573.0
23	7229.5
24	8886.0
25	13008.5
26	17131.0
27	27083.0
28	37035.0
29	47367.5
30	57700.0
31	68624.5
32	79549.0
33	98832.0
34	118115.0
35	138292.0
36	158469.0
37	156061.5
38	153654.0
39	136274.0
40	118894.0
41	102396.5
42	85899.0
43	72626.0
44	59353.0
45	57114.0
46	54875.0
47	54282.0
48	53689.0
49	53757.0
50	53825.0
51	43922.5
52	34020.0
53	32607.5
54	31195.0
55	35723.0
56	40251.0
57	41008.0
58	41765.0
59	37904.5
60	34044.0
61	29127.5
62	24211.0
63	20854.0
64	17497.0
65	15124.5
66	12752.0
67	12578.5
68	12405.0
69	13123.0
70	13841.0
71	10564.5
72	7288.0
73	6691.0
74	6094.0
75	4176.0
76	2258.0
77	2337.5
78	2417.0
79	2101.0
80	1785.0
81	1437.5
82	1090.0
83	1013.0
84	936.0
85	747.5
86	559.0
87	386.0
88	213.0
89	138.0
90	63.0
91	42.5
92	22.0
93	18.5
94	15.0
95	10.5
96	6.0
97	9.0
98	12.0
99	9.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032711159724549986
2	0.0054396085834083115
3	5.880657928008985E-4
4	0.0
5	0.0
6	1.4701644820022463E-4
7	0.0
8	0.0
9	0.0
10	7.350822410011232E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.675411205005616E-4
17	0.0
18	7.350822410011232E-5
19	0.0
20	1.4701644820022463E-4
21	0.0
22	0.0
23	0.0
24	5.880657928008985E-4
25	2.2052467230033697E-4
26	2.9403289640044926E-4
27	2.9403289640044926E-4
28	0.0012496398097019096
29	6.615740169010109E-4
30	0.0010291151374015725
31	5.145575687007862E-4
32	7.350822410011232E-4
33	2.9403289640044926E-4
34	5.880657928008985E-4
35	2.9403289640044926E-4
36	2.9403289640044926E-4
37	0.001617180930202471
38	0.0
39	7.350822410011232E-4
40	7.350822410011232E-5
41	0.0013966562579021341
42	0.0054396085834083115
43	0.0038959358773059533
44	0.003969444101406066
45	0.0017641973784026958
46	0.0021317384989032575
47	0.0014701644820022464
48	0.003969444101406066
49	0.004410493446006739
50	0.002352263171203594
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1360392.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.001534025110374
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.7721744326002	30.985444945372347
2	14.494136652915076	12.175519475840241
3	5.037436690223236	6.347402056312521
4	2.2904829773455737	3.8481519482766497
5	1.187912091915287	2.494706507370999
6	0.6858775902826184	1.728474656718967
7	0.44456707331736056	1.3070749339468002
8	0.32003857541881114	1.075368889184084
9	0.22790184126587673	0.8615004246282624
>10	1.2336858098788548	9.648305659456696
>50	0.13728046809044073	4.050817411292636
>100	0.13705372896078352	12.19167907954156
>500	0.021436604859710096	6.175580767446959
>1k	0.010015462926257997	7.1099732446114325
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	4675	0.3436509476680251	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	3541	0.26029262153849775	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	3496	0.2569847514539927	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	3440	0.2528682909043864	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2712	0.1993543037595046	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2427	0.17840445989097262	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2299	0.16899540720615822	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2244	0.16495245488065205	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	2205	0.16208563414074767	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2185	0.16061546965874543	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	2174	0.15980687919364417	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2156	0.15848373115984218	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2110	0.155102352851237	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	2073	0.15238254855953284	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2012	0.147898546889426	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC	1961	0.14414962746032026	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1951	0.14341454521931915	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1847	0.13576968991290747	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	1808	0.1329028691730031	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	1784	0.13113867179460037	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1783	0.13106516357050027	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	1676	0.12319978359178825	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG	1633	0.12003892995548342	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	1616	0.11878929014578152	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT	1589	0.11680456809507848	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT	1586	0.11658404342277813	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC	1525	0.11210004175267128	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG	1466	0.10776305653076466	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC	1419	0.10430816999805939	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA	1371	0.100779775241254	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT	1370	0.10070626701715389	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1368	0.10055925056895365	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	7.350822410011232E-5	0.0
9	0.0	0.0	0.0	7.350822410011232E-5	0.0
10	0.0	0.0	0.0	7.350822410011232E-5	0.0
11	0.0	0.0	0.0	7.350822410011232E-5	0.0
12	0.0	0.0	0.0	1.4701644820022463E-4	0.0
13	0.0	0.0	0.0	1.4701644820022463E-4	0.0
14	0.0	0.0	0.0	1.4701644820022463E-4	0.0
15	0.0	0.0	0.0	1.4701644820022463E-4	0.0
16	0.0	0.0	0.0	1.4701644820022463E-4	0.0
17	0.0	0.0	0.0	2.2052467230033697E-4	0.0
18	0.0	0.0	0.0	2.2052467230033697E-4	0.0
19	0.0	0.0	0.0	2.2052467230033697E-4	0.0
20	0.0	0.0	0.0	5.880657928008985E-4	0.0
21	0.0	0.0	0.0	0.0010291151374015725	0.0
22	0.0	0.0	0.0	0.0014701644820022464	0.0
23	0.0	0.0	0.0	0.002058230274803145	0.0
24	0.0	0.0	0.0	0.0026462960676040437	0.0
25	0.0	0.0	0.0	0.0033813783086051666	0.0
26	0.0	0.0	0.0	0.00411646054960629	0.0
27	0.0	0.0	0.0	0.00507206746290775	0.0
28	0.0	0.0	0.0	0.0063952154967097715	0.0
29	0.0	0.0	0.0	0.008306429323312691	0.0
30	0.0	0.0	0.0	0.013525513234420666	0.0
31	0.0	0.0	0.0	0.020949843868532013	0.0
32	0.0	0.0	0.0	0.029844338984645603	0.0
33	0.0	0.0	0.0	0.03778322718745773	0.0
34	0.0	0.0	0.0	0.04557509894206964	0.0
35	0.0	0.0	0.0	0.05344047892078166	0.0
36	0.0	0.0	0.0	0.06578986056960053	0.0
37	0.0	0.0	0.0	0.0877688195755341	0.0
38	0.0	0.0	0.0	0.11342318978647331	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGTGCG	25	0.002353263	35.198883	9
CCTCGCA	70	3.592504E-8	28.284817	30
CGGCACA	40	7.026341E-4	27.50418	42
AGACGGC	40	7.033894E-4	27.499126	6
GTATCAA	2095	0.0	26.361034	1
GTCCTAG	310	0.0	24.841572	1
AGGACCT	3230	0.0	23.838253	5
CGCTTAC	65	1.8140538E-5	23.691557	18
TAGGACC	2495	0.0	23.54234	4
GGACCTG	3205	0.0	23.406435	6
TGTAGGA	5705	0.0	23.29111	2
CTGTAGG	5510	0.0	23.120632	1
GTAAGAC	135	5.456968E-12	22.81409	3
GGTATCA	880	0.0	22.752623	1
GTAGGAC	5810	0.0	22.605135	3
CGACGCT	70	3.2165233E-5	21.999302	15
TAGAACG	90	4.0838313E-7	21.999302	4
ACGCTTA	70	3.2165233E-5	21.999302	17
GCGATAA	50	0.0025810788	21.999302	20
GACGCTT	70	3.2165233E-5	21.999302	16
>>END_MODULE