FastQCFastQC Report
Thu 2 Feb 2017
SRR4062222_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062222_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101199
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA58090.5275159167416608No Hit
GTCCTACAGTGGACATTTCTAAATT55800.5067204020345096No Hit
GTCCTAAAGTGTGTATTTCTCATTT54020.4905562028298246No Hit
CTTTAGGACGTGAAATATGGCGAGG48000.43588851787914806No Hit
GTCCTACAGTGTGCATTTCTCATTT39370.35751939476879296No Hit
GTATCAACGCAGAGTACTTTTTTTT37350.33917575297471214No Hit
CTGTAGGACCTGGAATATGGCGAGA27640.25099913821207614No Hit
TATCAACGCAGAGTACTTTTTTTTT23460.2130405131134336No Hit
ATTTAGAAATGTCCACTGTAGGACG19500.17707971038840392No Hit
CTGAAGGACCTGGAATATGGCGAGA19340.1756267486621401No Hit
GTCCTTCAGTGTGCATTTCTCATTT17750.1611879415073933No Hit
GAATATGGCAAGAAAACTGAAAATC16150.14665832424475503No Hit
GGTATCAACGCAGAGTACTTTTTTT14920.13548868097410185No Hit
TTTCTAAATTTTCCACCTTTTTCAG14700.1334908586004891No Hit
CTGTAGGACATGGAATATGGCAAGA12760.11587369766954018No Hit
GCGCAAGACGGACCAGAGCGAAAGC11760.10679268688039129No Hit
GGAATATGGCGAGAAAACTGAAAAT11730.1065202565567168No Hit
ATTCCAGGTCCTTCAGTGTGCATTT11520.10461324429099554No Hit
GAAATATGGCGAGGAAAACTGAAAA11040.10025435911220407No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAA350.002167265416.2884049
GACGTGT350.00216792416.2876637
ACGTGTA350.00216792416.2876638
TAGGGCC350.002169241416.286184
GGTCGGC551.1254606E-515.5487311
CGGCGTC551.1274027E-515.54590114
GGCGTCC551.12788875E-515.54519415
GCGTCCC551.1283748E-515.54448816
CGCAAGA2050.014.8256492
GGTATCA10100.014.3836541
GTCGGCG602.5618194E-514.25300112
CCGATCA400.00527202614.2523539
CCGACCT400.00527202614.2523539
TCGGCGT602.5651176E-514.25105813
CCAACGA602.572828E-514.24652219
AGGACGG551.958854E-413.8185795
CGCATCG1051.36788E-913.57243613
CAATGCT707.255776E-613.5718174
AAGACGG2250.013.5114985
TGGTCGT500.001497196313.30219710
Produced by FastQC (version 0.11.5)