##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062222_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1101199
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.314369155801995	32.0	32.0	32.0	32.0	32.0
2	31.01163913152845	32.0	32.0	32.0	32.0	32.0
3	31.034561418962422	32.0	32.0	32.0	32.0	32.0
4	31.014872879470467	32.0	32.0	32.0	32.0	32.0
5	31.050886352058075	32.0	32.0	32.0	32.0	32.0
6	34.69854494964125	36.0	36.0	36.0	32.0	36.0
7	34.60290828451533	36.0	36.0	36.0	32.0	36.0
8	34.5869874563998	36.0	36.0	36.0	32.0	36.0
9	34.61281929969061	36.0	36.0	36.0	32.0	36.0
10	34.43336762928408	36.0	36.0	36.0	32.0	36.0
11	34.628622074665884	36.0	36.0	36.0	32.0	36.0
12	34.49512304315569	36.0	36.0	36.0	32.0	36.0
13	34.54959367017224	36.0	36.0	36.0	32.0	36.0
14	34.51180031946996	36.0	36.0	36.0	32.0	36.0
15	34.50792454406515	36.0	36.0	36.0	32.0	36.0
16	34.495985739180654	36.0	36.0	36.0	32.0	36.0
17	34.434925930735496	36.0	36.0	36.0	32.0	36.0
18	34.455482614858894	36.0	36.0	36.0	32.0	36.0
19	34.39462168055002	36.0	36.0	36.0	32.0	36.0
20	34.368351224438086	36.0	36.0	36.0	32.0	36.0
21	34.33506205508723	36.0	36.0	36.0	32.0	36.0
22	34.28224780443862	36.0	36.0	36.0	32.0	36.0
23	34.31714885320455	36.0	36.0	36.0	32.0	36.0
24	34.26651041274102	36.0	36.0	36.0	32.0	36.0
25	33.95552847396338	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	11.0
5	82.0
6	221.0
7	31.0
8	179.0
9	162.0
10	81.0
11	29.0
12	57.0
13	46.0
14	199.0
15	279.0
16	403.0
17	550.0
18	636.0
19	894.0
20	1309.0
21	1920.0
22	2711.0
23	3738.0
24	5122.0
25	7346.0
26	9828.0
27	13191.0
28	17472.0
29	23524.0
30	31080.0
31	42732.0
32	61453.0
33	91579.0
34	220060.0
35	564274.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.08007511866697	20.664716656783426	12.892767921277962	24.36244030327164
2	12.887498081636469	21.11598357790009	45.13552954552265	20.860988794940788
3	17.800175627067635	27.46865456407891	29.781047351200556	24.9501224576529
4	10.391727522643327	17.518438430038408	40.35584512536134	31.733988921956925
5	11.009159310966918	40.79022788816248	35.7154990657194	12.485113735151206
6	29.243462300325294	39.951750958620316	19.074363494284626	11.730423246769767
7	27.25783504920343	32.825435785550326	22.647667098475036	17.26906206677121
8	24.06509090909091	39.77672727272727	20.271818181818183	15.886363636363637
9	27.218151616842036	14.96593753381017	21.466672606649205	36.3492382426986
10	15.441544872838243	29.073663708091267	33.68856849021724	21.796222928853247
11	33.38610702905615	22.579351511006998	26.84529092522807	17.189250534708783
12	24.919777440934464	25.609386835043868	32.02000812909808	17.450827594923588
13	28.685268382810914	23.50801197682105	26.74380859636058	21.06291104400745
14	19.700073186142816	25.059117129648573	27.7743684854105	27.46644119879811
15	20.777222708185953	36.3410446987621	23.165193923776233	19.716538669275714
16	19.638244773459768	28.939327129823667	31.774818963283526	19.64760913343304
17	19.080111356185235	31.61668306218599	29.327644300268936	19.97556128135984
18	19.388024124118846	27.69037198925797	34.563873095643736	18.357730790979456
19	23.44128929045394	26.63079244459796	28.571000054538516	21.356918210409585
20	24.290252359766047	27.634871366051183	29.372259058456756	18.70261721572602
21	22.81295417176793	26.98940299959008	26.883060341732584	23.314582486909398
22	23.259578962869504	31.102097731737228	27.549398096342763	18.088925209050508
23	19.343449123259834	30.92665264657929	30.24403676728533	19.485861462875548
24	22.529619480005998	27.54713894228803	31.06284737844311	18.860394199262863
25	20.336883396863655	29.358825443394394	30.639065967924004	19.665225191817946
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	493.0
1	493.0
2	538.5
3	584.0
4	584.0
5	584.0
6	1267.5
7	1951.0
8	1951.0
9	1951.0
10	2456.5
11	2962.0
12	2962.0
13	2962.0
14	4262.0
15	5562.0
16	5562.0
17	5562.0
18	9837.5
19	14113.0
20	14113.0
21	14113.0
22	24700.0
23	35287.0
24	35287.0
25	35287.0
26	53379.5
27	71472.0
28	71472.0
29	71472.0
30	94121.5
31	116771.0
32	116771.0
33	116771.0
34	129647.0
35	142523.0
36	142523.0
37	142523.0
38	144681.0
39	146839.0
40	146839.0
41	146839.0
42	145395.0
43	143951.0
44	143951.0
45	143951.0
46	141538.0
47	139125.0
48	139125.0
49	139125.0
50	122853.0
51	106581.0
52	106581.0
53	106581.0
54	83433.0
55	60285.0
56	60285.0
57	60285.0
58	50495.5
59	40706.0
60	40706.0
61	40706.0
62	34658.5
63	28611.0
64	28611.0
65	28611.0
66	23662.0
67	18713.0
68	18713.0
69	18713.0
70	14348.5
71	9984.0
72	9984.0
73	9984.0
74	8130.5
75	6277.0
76	6277.0
77	6277.0
78	5489.5
79	4702.0
80	4702.0
81	4702.0
82	3318.5
83	1935.0
84	1935.0
85	1935.0
86	1464.5
87	994.0
88	994.0
89	994.0
90	665.5
91	337.0
92	337.0
93	337.0
94	201.5
95	66.0
96	66.0
97	66.0
98	220.5
99	375.0
100	375.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.264808631319134E-4
2	0.0
3	1.8162021578297836E-4
4	0.009262631004931897
5	0.03196515797780419
6	0.05984386110049137
7	0.1066110666646083
8	0.10888131936189555
9	0.11877962112206786
10	0.13240113730579123
11	0.13939351561343588
12	0.13131141601109336
13	0.12786063191121677
14	0.11478397637484233
15	0.13049412504006996
16	0.11660017853267213
17	0.11850719079839339
18	0.11087914173550831
19	0.09616790425708704
20	0.08935714616522536
21	0.08917552594944238
22	0.10043597932798703
23	0.07927722418927007
24	0.09026524724414026
25	0.08508907109432537
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1101199.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.27929364004956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.24755963085238	34.824039078533566
2	14.340408721797907	13.273279723088264
3	4.637178373836822	6.438160188722454
4	1.946817409002767	3.603893381391981
5	0.9708586328287577	2.246532587582957
6	0.5720405060970295	1.5884178333400194
7	0.37226388282992995	1.2059676681549933
8	0.2582032544860724	0.9559571386541911
9	0.19450072855300726	0.8101220696917375
>10	1.1840421576188367	10.554337665543443
>50	0.13826751891536668	4.447848970299029
>100	0.12247575524612074	11.463600755291578
>500	0.011044512363579225	3.6889143284671952
>1k	0.00374724526621438	3.366353381396652
>5k	5.916703051917443E-4	1.5325752298419604
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGA	5809	0.5275159167416608	No Hit
GTCCTACAGTGGACATTTCTAAATT	5580	0.5067204020345096	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5402	0.4905562028298246	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4800	0.43588851787914806	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3937	0.35751939476879296	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3735	0.33917575297471214	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2764	0.25099913821207614	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2346	0.2130405131134336	No Hit
ATTTAGAAATGTCCACTGTAGGACG	1950	0.17707971038840392	No Hit
CTGAAGGACCTGGAATATGGCGAGA	1934	0.1756267486621401	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	1775	0.1611879415073933	No Hit
GAATATGGCAAGAAAACTGAAAATC	1615	0.14665832424475503	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1492	0.13548868097410185	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	1470	0.1334908586004891	No Hit
CTGTAGGACATGGAATATGGCAAGA	1276	0.11587369766954018	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1176	0.10679268688039129	No Hit
GGAATATGGCGAGAAAACTGAAAAT	1173	0.1065202565567168	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	1152	0.10461324429099554	No Hit
GAAATATGGCGAGGAAAACTGAAAA	1104	0.10025435911220407	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGTAA	35	0.0021672654	16.288404	9
GACGTGT	35	0.002167924	16.287663	7
ACGTGTA	35	0.002167924	16.287663	8
TAGGGCC	35	0.0021692414	16.28618	4
GGTCGGC	55	1.1254606E-5	15.54873	11
CGGCGTC	55	1.1274027E-5	15.545901	14
GGCGTCC	55	1.12788875E-5	15.545194	15
GCGTCCC	55	1.1283748E-5	15.544488	16
CGCAAGA	205	0.0	14.825649	2
GGTATCA	1010	0.0	14.383654	1
GTCGGCG	60	2.5618194E-5	14.253001	12
CCGATCA	40	0.005272026	14.252353	9
CCGACCT	40	0.005272026	14.252353	9
TCGGCGT	60	2.5651176E-5	14.251058	13
CCAACGA	60	2.572828E-5	14.246522	19
AGGACGG	55	1.958854E-4	13.818579	5
CGCATCG	105	1.36788E-9	13.572436	13
CAATGCT	70	7.255776E-6	13.571817	4
AAGACGG	225	0.0	13.511498	5
TGGTCGT	50	0.0014971963	13.302197	10
>>END_MODULE