FastQCFastQC Report
Thu 2 Feb 2017
SRR4062222_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062222_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101199
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA29550.2683438688193506No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC22430.20368707200061026No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA21470.19496930164302728No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA19770.17953158330147412No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12350.11215048324598915No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT11280.10243380170159981No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGGG250.002353095235.19880320
CGCTATC300.0057457529.33233612
GTATCAA28550.027.2886811
GGTATCA11150.026.2519871
GACCCGG1400.023.5706256
CTCTAGG2650.023.2539921
CTAGGAC2650.023.2444923
ATTGCGA952.6857379E-823.15710617
TAGGACC14300.022.9222974
AGTGACG1355.456968E-1222.8140419
TAGGACA8650.022.3807414
TGTAGGA34950.022.0347252
ACGGGAT602.870568E-422.00224743
CAATACG2100.022.0002538
CTCGAAT602.8732143E-421.99925210
CGACCCG1600.021.999255
AGGACCT19600.021.5502875
AATACGA2150.021.48959239
GTCCTAC31400.021.4475251
CTGTAGG34500.021.3703231
Produced by FastQC (version 0.11.5)