##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062222_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1101199
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.308081463931586	32.0	32.0	32.0	32.0	32.0
2	31.553631087569094	32.0	32.0	32.0	32.0	32.0
3	31.641528007199426	32.0	32.0	32.0	32.0	32.0
4	31.702279969378832	32.0	32.0	32.0	32.0	32.0
5	31.701968490708765	32.0	32.0	32.0	32.0	32.0
6	35.35420845823507	36.0	36.0	36.0	36.0	36.0
7	35.35339207536512	36.0	36.0	36.0	36.0	36.0
8	35.31678561277299	36.0	36.0	36.0	36.0	36.0
9	35.37576223734312	36.0	36.0	36.0	36.0	36.0
10	35.28655492785591	36.0	36.0	36.0	36.0	36.0
11	35.381929151770024	36.0	36.0	36.0	36.0	36.0
12	35.31288895104336	36.0	36.0	36.0	36.0	36.0
13	35.336770193216665	36.0	36.0	36.0	36.0	36.0
14	35.31059690392018	36.0	36.0	36.0	36.0	36.0
15	35.30497939064601	36.0	36.0	36.0	36.0	36.0
16	35.318588193414634	36.0	36.0	36.0	36.0	36.0
17	35.30678651179306	36.0	36.0	36.0	36.0	36.0
18	35.304677901087814	36.0	36.0	36.0	36.0	36.0
19	35.27064045644793	36.0	36.0	36.0	36.0	36.0
20	35.26262918872974	36.0	36.0	36.0	36.0	36.0
21	35.23993937517197	36.0	36.0	36.0	36.0	36.0
22	35.202973304552586	36.0	36.0	36.0	36.0	36.0
23	35.205605889580355	36.0	36.0	36.0	36.0	36.0
24	35.18289064919238	36.0	36.0	36.0	36.0	36.0
25	35.143202999639485	36.0	36.0	36.0	36.0	36.0
26	35.13388315826658	36.0	36.0	36.0	36.0	36.0
27	35.07479029675835	36.0	36.0	36.0	36.0	36.0
28	35.03132585481825	36.0	36.0	36.0	36.0	36.0
29	34.99992281140829	36.0	36.0	36.0	36.0	36.0
30	34.94834539442916	36.0	36.0	36.0	36.0	36.0
31	34.95252538369541	36.0	36.0	36.0	36.0	36.0
32	34.91387569367571	36.0	36.0	36.0	36.0	36.0
33	34.94219573392275	36.0	36.0	36.0	36.0	36.0
34	34.919432364177595	36.0	36.0	36.0	36.0	36.0
35	34.908316298870595	36.0	36.0	36.0	36.0	36.0
36	34.84770963286382	36.0	36.0	36.0	36.0	36.0
37	34.74300376226277	36.0	36.0	36.0	32.0	36.0
38	34.728518641952995	36.0	36.0	36.0	32.0	36.0
39	34.53991876127748	36.0	36.0	36.0	32.0	36.0
40	34.63057903249095	36.0	36.0	36.0	32.0	36.0
41	34.604467494067826	36.0	36.0	36.0	32.0	36.0
42	34.598050851844214	36.0	36.0	36.0	32.0	36.0
43	34.60140537722973	36.0	36.0	36.0	32.0	36.0
44	34.4789288766154	36.0	36.0	36.0	32.0	36.0
45	34.45028282808103	36.0	36.0	36.0	32.0	36.0
46	34.292300483382206	36.0	36.0	36.0	32.0	36.0
47	34.30258381999984	36.0	36.0	36.0	32.0	36.0
48	34.25814407750098	36.0	36.0	36.0	32.0	36.0
49	34.19496566923871	36.0	36.0	36.0	32.0	36.0
50	33.881668072709836	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	8.0
21	23.0
22	69.0
23	185.0
24	504.0
25	1191.0
26	2591.0
27	5182.0
28	9210.0
29	14857.0
30	22454.0
31	32808.0
32	48991.0
33	79966.0
34	172739.0
35	710419.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.63745635167199	21.264502508984016	13.328409838269545	24.769631301074448
2	12.48840966531745	21.62654284269021	44.65555023780052	21.229497254191816
3	17.34710742300658	27.820791537943222	30.091591157993385	24.740509881056813
4	10.27526384900808	17.78790008699616	40.611497659821396	31.325338404174364
5	10.767899353341221	41.09466136456717	35.53281468653713	12.604624595554482
6	29.307108537346178	40.148038906753285	18.83405058313817	11.710801972762367
7	27.269730539166854	33.00656829510379	22.532984501438886	17.19071666429047
8	24.10654205098261	39.79516872063996	20.169833063778665	15.92845616459877
9	27.116352266938126	14.774986174161073	21.465148442742866	36.64351311615793
10	15.432556179724264	29.05699065926382	33.61202980753688	21.89842335347503
11	33.40195550486334	22.516003011263177	26.922200256266127	17.159841227607362
12	24.976866125014645	25.509558217906118	32.019734852646984	17.49384080443226
13	28.793342529370257	23.329934008294593	26.815589189601518	21.06113427273363
14	19.870886188599883	24.977501795769882	27.72450755948743	27.427104456142803
15	20.757011221405033	36.34956079691318	23.135237136975242	19.75819084470654
16	19.774081587803785	29.033667092265304	31.4158994691217	19.776351850809213
17	19.072302099802123	31.792346342486688	29.153586227375794	19.98176533033539
18	19.507391041393102	27.497507260274716	34.653895121494955	18.341206576837227
19	23.70516137410223	26.409213956787102	28.663665695301212	21.221958973809457
20	24.494390185207887	27.442641857255072	29.383624153760234	18.679343803776806
21	22.896497363328518	26.786166714644672	27.035168030483135	23.282167891543672
22	23.46560430948448	30.843925575667974	27.666207470221092	18.02426264462645
23	19.38550616191987	30.718426006561938	30.21860717272718	19.67746065879101
24	22.706274763006167	27.371504800719947	30.97860585240136	18.943614583872524
25	20.467436373840126	29.164691545026418	30.606575747504085	19.761296333629375
26	18.135339875299447	31.08678003410831	31.344226923768474	19.43365316682377
27	19.725171790333608	30.23709654130914	29.70358618848399	20.334145479873264
28	18.728307321795388	30.36611701287167	28.98078239848598	21.924793266846958
29	20.56899952415374	28.72340618745437	28.590187396249167	22.117406892142725
30	19.132626883535675	28.666248330210948	30.760443049182385	21.440681737070996
31	22.758953244344994	27.390112359958703	29.09889547063957	20.752038925056734
32	19.97074077067466	29.8763793020466	29.50278380408122	20.65009612319752
33	18.44509343460486	30.602761719509115	31.524575961816133	19.427568884069895
34	20.34428240747047	29.847192443803927	31.386437750467906	18.422087398257702
35	20.04974591262591	30.367800100981114	30.593191402802045	18.989262583590932
36	18.475235494595406	29.467768138736805	32.02563038450118	20.031365982166612
37	19.845619451949055	29.132699162258497	28.513088292051673	22.508593093740778
38	20.021812587915537	31.21797241007302	28.67692397105337	20.083291030958073
39	22.46699715672003	28.089263190013014	28.93016937085506	20.513570282411894
40	23.16377254590001	27.799178712638415	30.37210383600406	18.66494490545751
41	22.58982587864357	27.520782138530635	30.91484998901185	18.974541993813943
42	21.205519310685535	30.807463456452876	29.96752491935718	18.019492313504404
43	19.248226409337096	30.630264921591493	30.731340518509636	19.390168150561774
44	18.670968843425225	30.690515716325145	29.77138404646787	20.86713139378176
45	19.21826855229569	31.133039282727204	29.266753301712452	20.38193886326465
46	19.89596501169667	28.48756956250091	29.913328393124395	21.70313703267803
47	22.48816732474382	28.018013272937953	29.35157302840205	20.14224637391617
48	19.867902698795266	30.067074843028884	28.59271784793826	21.472304610237586
49	21.692136684453907	28.78590291727663	28.318942500844564	21.203017897424907
50	19.68246876313928	31.99731561420763	27.900812126594758	20.41940349605833
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	160.0
1	94.0
2	28.0
3	98.5
4	169.0
5	214.0
6	259.0
7	226.5
8	194.0
9	275.0
10	356.0
11	583.5
12	811.0
13	1535.5
14	2260.0
15	3150.0
16	4040.0
17	4221.5
18	4403.0
19	4468.0
20	4533.0
21	5284.5
22	6036.0
23	7380.5
24	8725.0
25	11641.0
26	14557.0
27	20096.5
28	25636.0
29	32950.0
30	40264.0
31	50511.0
32	60758.0
33	74251.5
34	87745.0
35	101703.5
36	115662.0
37	117768.5
38	119875.0
39	111202.0
40	102529.0
41	93564.0
42	84599.0
43	76243.5
44	67888.0
45	63835.0
46	59782.0
47	56214.0
48	52646.0
49	49606.0
50	46566.0
51	40416.5
52	34267.0
53	31075.5
54	27884.0
55	28175.0
56	28466.0
57	27157.5
58	25849.0
59	22836.0
60	19823.0
61	16935.5
62	14048.0
63	11921.5
64	9795.0
65	8403.0
66	7011.0
67	6751.5
68	6492.0
69	6548.5
70	6605.0
71	5068.5
72	3532.0
73	3152.5
74	2773.0
75	1961.0
76	1149.0
77	1113.5
78	1078.0
79	930.5
80	783.0
81	618.0
82	453.0
83	401.5
84	350.0
85	290.5
86	231.0
87	154.0
88	77.0
89	50.0
90	23.0
91	14.5
92	6.0
93	7.5
94	9.0
95	8.5
96	8.0
97	6.0
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032237588301478666
2	0.005993467120838287
3	9.081010789148918E-5
4	9.081010789148918E-5
5	0.0
6	1.8162021578297836E-4
7	0.0
8	0.0
9	0.0
10	9.081010789148918E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.5405053945744595E-4
17	0.0
18	9.081010789148918E-5
19	0.0
20	3.632404315659567E-4
21	0.0
22	0.0
23	0.0
24	9.081010789148919E-4
25	9.081010789148918E-5
26	9.081010789148918E-5
27	1.8162021578297836E-4
28	0.0019070122657212731
29	2.7243032367446754E-4
30	0.0010897212946978701
31	5.448606473489351E-4
32	7.264808631319134E-4
33	9.081010789148918E-5
34	9.081010789148919E-4
35	2.7243032367446754E-4
36	5.448606473489351E-4
37	0.0021794425893957403
38	0.0
39	5.448606473489351E-4
40	9.081010789148918E-5
41	0.0011805314025893595
42	0.004268075070899992
43	0.003904834639334035
44	0.004631315502465949
45	0.0025426830209616972
46	0.002815113344636165
47	9.98911186806381E-4
48	0.0037232144235510565
49	0.0031783537762021218
50	0.0014529617262638269
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1101199.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.95782784623825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.16648315414494	41.85944468838903
2	14.353939750188912	15.777226994123167
3	4.510566631952244	7.4367283334345435
4	1.8205312185639118	4.002097651941512
5	0.8952644617116355	2.460089508180161
6	0.5001889411240387	1.649357863013227
7	0.3213584609277481	1.2362814080819484
8	0.21584135074776867	0.948973743719632
9	0.171365364932482	0.8476081402290426
>10	0.8727285523548267	9.098270958134162
>50	0.0920385883432636	3.540437231461363
>100	0.07303853462565194	7.885986184192751
>500	0.005323992306056777	1.9951325149910197
>1k	0.0013309980765141942	1.2623647801084337
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	2955	0.2683438688193506	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	2243	0.20368707200061026	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	2147	0.19496930164302728	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	1977	0.17953158330147412	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1235	0.11215048324598915	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1128	0.10243380170159981	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	9.081010789148919E-5	0.0
21	0.0	0.0	0.0	9.081010789148919E-5	0.0
22	0.0	0.0	0.0	1.8162021578297839E-4	0.0
23	0.0	0.0	0.0	4.5405053945744595E-4	0.0
24	0.0	0.0	0.0	5.448606473489351E-4	0.0
25	0.0	0.0	0.0	6.356707552404243E-4	0.0
26	0.0	0.0	0.0	7.264808631319135E-4	0.0
27	0.0	0.0	0.0	8.172909710234027E-4	0.0
28	0.0	0.0	0.0	0.0011805314025893595	0.0
29	0.0	0.0	0.0	0.0017253920499382945	0.0
30	0.0	0.0	0.0	0.0035415942077680783	0.0
31	0.0	0.0	0.0	0.008263719818125516	0.0
32	0.0	0.0	0.0	0.014438807154746781	0.0
33	0.0	0.0	0.0	0.020613894491368044	0.0
34	0.0	0.0	0.0	0.027424652583229733	0.0
35	0.0	0.0	0.0	0.036324043156595674	0.0
36	0.0	0.0	0.0	0.048674217829838204	0.0
37	0.0	0.0	0.0	0.06783515059494243	0.0
38	0.0	0.0	0.0	0.09289874037299344	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCGGG	25	0.0023530952	35.198803	20
CGCTATC	30	0.00574575	29.332336	12
GTATCAA	2855	0.0	27.288681	1
GGTATCA	1115	0.0	26.251987	1
GACCCGG	140	0.0	23.570625	6
CTCTAGG	265	0.0	23.253992	1
CTAGGAC	265	0.0	23.244492	3
ATTGCGA	95	2.6857379E-8	23.157106	17
TAGGACC	1430	0.0	22.922297	4
AGTGACG	135	5.456968E-12	22.81404	19
TAGGACA	865	0.0	22.380741	4
TGTAGGA	3495	0.0	22.034725	2
ACGGGAT	60	2.870568E-4	22.002247	43
CAATACG	210	0.0	22.00025	38
CTCGAAT	60	2.8732143E-4	21.999252	10
CGACCCG	160	0.0	21.99925	5
AGGACCT	1960	0.0	21.550287	5
AATACGA	215	0.0	21.489592	39
GTCCTAC	3140	0.0	21.447525	1
CTGTAGG	3450	0.0	21.370323	1
>>END_MODULE