FastQCFastQC Report
Thu 2 Feb 2017
SRR4062221_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062221_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1980
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTT120.6060606060606061No Hit
GTATCAACGCAGAGTACTTTTTTTT90.45454545454545453No Hit
ACGCAGAGTACTTTTTTTTTTTTTT80.40404040404040403No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG60.30303030303030304No Hit
GGTATCAACGCAGAGTACTTTTTTT50.25252525252525254No Hit
CCCCGAGACCATCTAACAGCACTTT40.20202020202020202No Hit
CTCCATGACCATCAGACGGATCTCC40.20202020202020202No Hit
GTTCACAGGAACCTGCTGCTCCAGC30.15151515151515152No Hit
GAGTACTTTTTTTTTTTTTTTTTTT30.15151515151515152No Hit
CCATTGAATATGGCTGATAAAAACT30.15151515151515152No Hit
TTCGGTCACAGAGCCGATCTGGTTC30.15151515151515152No Hit
CTCTGGCCCTGCACCACGCAAGTCT20.10101010101010101No Hit
GCTGCCAATCACCCCGGAGCCAGGG20.10101010101010101No Hit
GTATCAACGCAGAGTACATGGGGGG20.10101010101010101No Hit
CTCAATAATGAGCTGAGTATCAAAG20.10101010101010101No Hit
CTCCAATTCGATCACAGTAGGACAT20.10101010101010101No Hit
GTCGTGGTGCCGTCTTCTGTGTGAA20.10101010101010101No Hit
CTGTAAAGGGGTCGAAAAGCATAGC20.10101010101010101No Hit
GTCCAGGACAAGGTCAATGATCTCC20.10101010101010101No Hit
TCTCTGTACCGGTAACACATCTCCT20.10101010101010101No Hit
ACACAGTAGTTGCCTGCAGGGATCT20.10101010101010101No Hit
GTGTTCTTCCATCTAGTTCCCGGAC20.10101010101010101No Hit
GCTTTACACAACTTAACAAGGCTGT20.10101010101010101No Hit
CTATAGAACAGTTTCTCATTTCTTT20.10101010101010101No Hit
GACCACACCCTTCTCGTCTGAGCTG20.10101010101010101No Hit
CCTCTTACCAGGGAGGGAGGCAGCC20.10101010101010101No Hit
ACTTTCTACCATGTCTGGACGCGGC20.10101010101010101No Hit
CTCTAGAGGACTGATGGCCATCTTC20.10101010101010101No Hit
CTTTATGTCGGCCTCTGAAGCTGTA20.10101010101010101No Hit
AGTGTACAGAGACCCAAAAACAATG20.10101010101010101No Hit
CCCAAGAGCAGGTCTGGGGCTGTTA20.10101010101010101No Hit
GGGCCTTAGTGGACCACGAGAATGT20.10101010101010101No Hit
CTTTCAATATCATCCTGCAGGATTC20.10101010101010101No Hit
CCCCTGTGCAGCACCCCTACTTCCC20.10101010101010101No Hit
CCTTAAAAGAATCATTTGAGGGCTC20.10101010101010101No Hit
ACTGAAACCTGCCCAGGGAACAACA20.10101010101010101No Hit
GTTTTCTGGTGATTTCTTTATGTCG20.10101010101010101No Hit
CTCTAATACAGCATCTGGAAGCAGC20.10101010101010101No Hit
GGGTAATGGGGGTGAACCTCGTAAG20.10101010101010101No Hit
ATGGTGTAGTGGCCACGAGCATAGT20.10101010101010101No Hit
TCTGAGACCCCTGCACTGAGCACTG20.10101010101010101No Hit
GTTGTAGCCATCATTATATTTCCTT20.10101010101010101No Hit
ATTTTAAGGAGAAACACAGTAGTTG20.10101010101010101No Hit
CAGTTCTGACTGCGCCTTCTCCAGG20.10101010101010101No Hit
ACTGTGGCCACAGTAAGCCCCACCT20.10101010101010101No Hit
GGCCAGGCGTCGCTTCTGCAAGCAG20.10101010101010101No Hit
CTTCTGCAAGCAGCCCCTGGGGCCC20.10101010101010101No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)