##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062221_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1980
Sequences flagged as poor quality	0
Sequence length	25
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.433333333333334	32.0	32.0	32.0	14.0	32.0
2	26.343939393939394	32.0	14.0	32.0	14.0	32.0
3	26.657575757575756	32.0	21.0	32.0	14.0	32.0
4	26.275757575757577	32.0	14.0	32.0	14.0	32.0
5	26.523232323232325	32.0	21.0	32.0	14.0	32.0
6	29.82323232323232	36.0	21.0	36.0	14.0	36.0
7	29.455555555555556	36.0	21.0	36.0	14.0	36.0
8	29.052020202020202	36.0	21.0	36.0	14.0	36.0
9	29.300505050505052	36.0	21.0	36.0	14.0	36.0
10	28.310606060606062	32.0	21.0	36.0	14.0	36.0
11	30.1010101010101	36.0	21.0	36.0	14.0	36.0
12	28.5510101010101	32.0	21.0	36.0	14.0	36.0
13	29.63282828282828	36.0	21.0	36.0	14.0	36.0
14	29.03030303030303	32.0	21.0	36.0	14.0	36.0
15	28.86969696969697	32.0	21.0	36.0	14.0	36.0
16	29.12979797979798	36.0	21.0	36.0	14.0	36.0
17	28.905050505050504	32.0	21.0	36.0	14.0	36.0
18	28.611616161616162	32.0	21.0	36.0	14.0	36.0
19	28.990909090909092	32.0	21.0	36.0	14.0	36.0
20	28.461111111111112	32.0	21.0	36.0	14.0	36.0
21	28.82929292929293	36.0	21.0	36.0	14.0	36.0
22	28.33888888888889	32.0	21.0	36.0	14.0	36.0
23	28.09292929292929	32.0	14.0	36.0	14.0	36.0
24	28.518686868686867	32.0	21.0	36.0	14.0	36.0
25	27.606565656565657	32.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	6.0
16	15.0
17	34.0
18	41.0
19	58.0
20	80.0
21	87.0
22	81.0
23	79.0
24	86.0
25	92.0
26	81.0
27	95.0
28	93.0
29	111.0
30	130.0
31	104.0
32	149.0
33	164.0
34	199.0
35	193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.54545454545455	18.333333333333332	12.222222222222221	29.898989898989896
2	17.87878787878788	18.13131313131313	37.07070707070707	26.91919191919192
3	20.05050505050505	20.404040404040405	27.22222222222222	32.323232323232325
4	14.242424242424242	14.7979797979798	31.515151515151512	39.44444444444444
5	15.757575757575756	35.858585858585855	30.2020202020202	18.181818181818183
6	38.43434343434343	30.1010101010101	15.454545454545453	16.01010101010101
7	32.42424242424242	28.98989898989899	19.545454545454547	19.04040404040404
8	28.484848484848484	33.03030303030303	17.02020202020202	21.464646464646464
9	25.202020202020204	14.292929292929294	16.565656565656568	43.93939393939394
10	15.909090909090908	28.18181818181818	28.939393939393938	26.969696969696972
11	38.98989898989899	20.303030303030305	18.939393939393938	21.767676767676765
12	25.265285497726126	24.25467407781708	27.438100050530572	23.041940373926227
13	30.151515151515152	16.565656565656568	25.5050505050505	27.77777777777778
14	22.32323232323232	16.767676767676768	27.32323232323232	33.58585858585859
15	23.585858585858585	26.86868686868687	20.606060606060606	28.939393939393938
16	25.90909090909091	25.757575757575758	23.03030303030303	25.303030303030305
17	22.637695805962608	24.153612935826175	25.77059120768065	27.438100050530572
18	25.252525252525253	24.141414141414142	25.90909090909091	24.6969696969697
19	26.91919191919192	22.474747474747474	25.40404040404041	25.202020202020204
20	24.242424242424242	23.83838383838384	25.808080808080806	26.111111111111114
21	26.060606060606062	23.636363636363637	26.565656565656564	23.737373737373737
22	25.063163213744318	24.456796361798887	25.063163213744318	25.416877210712478
23	24.292929292929294	25.252525252525253	23.98989898989899	26.464646464646464
24	24.747474747474747	24.747474747474747	25.858585858585858	24.646464646464647
25	23.98989898989899	21.71717171717172	26.262626262626267	28.030303030303028
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.0
9	1.0
10	3.5
11	6.0
12	6.0
13	6.0
14	4.5
15	3.0
16	3.0
17	3.0
18	6.0
19	9.0
20	9.0
21	9.0
22	15.5
23	22.0
24	22.0
25	22.0
26	39.5
27	57.0
28	57.0
29	57.0
30	60.5
31	64.0
32	64.0
33	64.0
34	82.0
35	100.0
36	100.0
37	100.0
38	119.5
39	139.0
40	139.0
41	139.0
42	165.5
43	192.0
44	192.0
45	192.0
46	211.0
47	230.0
48	230.0
49	230.0
50	249.5
51	269.0
52	269.0
53	269.0
54	260.0
55	251.0
56	251.0
57	251.0
58	237.0
59	223.0
60	223.0
61	223.0
62	191.0
63	159.0
64	159.0
65	159.0
66	139.0
67	119.0
68	119.0
69	119.0
70	92.0
71	65.0
72	65.0
73	65.0
74	51.0
75	37.0
76	37.0
77	37.0
78	26.0
79	15.0
80	15.0
81	15.0
82	10.5
83	6.0
84	6.0
85	6.0
86	5.5
87	5.0
88	5.0
89	5.0
90	3.0
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	3.5
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.050505050505050504
13	0.0
14	0.0
15	0.0
16	0.0
17	0.050505050505050504
18	0.0
19	0.0
20	0.0
21	0.0
22	0.050505050505050504
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1980.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.70707070707071
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.5197889182058	93.33333333333333
2	1.8997361477572559	3.6363636363636362
3	0.21108179419525064	0.6060606060606061
4	0.10554089709762532	0.40404040404040403
5	0.05277044854881266	0.25252525252525254
6	0.05277044854881266	0.30303030303030304
7	0.0	0.0
8	0.05277044854881266	0.40404040404040403
9	0.05277044854881266	0.45454545454545453
>10	0.05277044854881266	0.6060606060606061
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTT	12	0.6060606060606061	No Hit
GTATCAACGCAGAGTACTTTTTTTT	9	0.45454545454545453	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	8	0.40404040404040403	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	6	0.30303030303030304	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5	0.25252525252525254	No Hit
CCCCGAGACCATCTAACAGCACTTT	4	0.20202020202020202	No Hit
CTCCATGACCATCAGACGGATCTCC	4	0.20202020202020202	No Hit
GTTCACAGGAACCTGCTGCTCCAGC	3	0.15151515151515152	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3	0.15151515151515152	No Hit
CCATTGAATATGGCTGATAAAAACT	3	0.15151515151515152	No Hit
TTCGGTCACAGAGCCGATCTGGTTC	3	0.15151515151515152	No Hit
CTCTGGCCCTGCACCACGCAAGTCT	2	0.10101010101010101	No Hit
GCTGCCAATCACCCCGGAGCCAGGG	2	0.10101010101010101	No Hit
GTATCAACGCAGAGTACATGGGGGG	2	0.10101010101010101	No Hit
CTCAATAATGAGCTGAGTATCAAAG	2	0.10101010101010101	No Hit
CTCCAATTCGATCACAGTAGGACAT	2	0.10101010101010101	No Hit
GTCGTGGTGCCGTCTTCTGTGTGAA	2	0.10101010101010101	No Hit
CTGTAAAGGGGTCGAAAAGCATAGC	2	0.10101010101010101	No Hit
GTCCAGGACAAGGTCAATGATCTCC	2	0.10101010101010101	No Hit
TCTCTGTACCGGTAACACATCTCCT	2	0.10101010101010101	No Hit
ACACAGTAGTTGCCTGCAGGGATCT	2	0.10101010101010101	No Hit
GTGTTCTTCCATCTAGTTCCCGGAC	2	0.10101010101010101	No Hit
GCTTTACACAACTTAACAAGGCTGT	2	0.10101010101010101	No Hit
CTATAGAACAGTTTCTCATTTCTTT	2	0.10101010101010101	No Hit
GACCACACCCTTCTCGTCTGAGCTG	2	0.10101010101010101	No Hit
CCTCTTACCAGGGAGGGAGGCAGCC	2	0.10101010101010101	No Hit
ACTTTCTACCATGTCTGGACGCGGC	2	0.10101010101010101	No Hit
CTCTAGAGGACTGATGGCCATCTTC	2	0.10101010101010101	No Hit
CTTTATGTCGGCCTCTGAAGCTGTA	2	0.10101010101010101	No Hit
AGTGTACAGAGACCCAAAAACAATG	2	0.10101010101010101	No Hit
CCCAAGAGCAGGTCTGGGGCTGTTA	2	0.10101010101010101	No Hit
GGGCCTTAGTGGACCACGAGAATGT	2	0.10101010101010101	No Hit
CTTTCAATATCATCCTGCAGGATTC	2	0.10101010101010101	No Hit
CCCCTGTGCAGCACCCCTACTTCCC	2	0.10101010101010101	No Hit
CCTTAAAAGAATCATTTGAGGGCTC	2	0.10101010101010101	No Hit
ACTGAAACCTGCCCAGGGAACAACA	2	0.10101010101010101	No Hit
GTTTTCTGGTGATTTCTTTATGTCG	2	0.10101010101010101	No Hit
CTCTAATACAGCATCTGGAAGCAGC	2	0.10101010101010101	No Hit
GGGTAATGGGGGTGAACCTCGTAAG	2	0.10101010101010101	No Hit
ATGGTGTAGTGGCCACGAGCATAGT	2	0.10101010101010101	No Hit
TCTGAGACCCCTGCACTGAGCACTG	2	0.10101010101010101	No Hit
GTTGTAGCCATCATTATATTTCCTT	2	0.10101010101010101	No Hit
ATTTTAAGGAGAAACACAGTAGTTG	2	0.10101010101010101	No Hit
CAGTTCTGACTGCGCCTTCTCCAGG	2	0.10101010101010101	No Hit
ACTGTGGCCACAGTAAGCCCCACCT	2	0.10101010101010101	No Hit
GGCCAGGCGTCGCTTCTGCAAGCAG	2	0.10101010101010101	No Hit
CTTCTGCAAGCAGCCCCTGGGGCCC	2	0.10101010101010101	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE