FastQCFastQC Report
Thu 2 Feb 2017
SRR4062221_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062221_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1980
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140.7070707070707071No Hit
CTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCTC80.40404040404040403No Hit
GCCTAGCAGTGGCGCGGCCGCCGAGCCCCTGGACGAGGAGGAGGACGCGG60.30303030303030304No Hit
GCACCTGCCCCCGCCGCCGGGCCCGGACAGATGTCCTTCACCTTTGCCTC40.20202020202020202No Hit
CCTTGTACCTTGACCTGCCGCCGAGTGCACCTGAAAGAGAAGGGCAAGCC40.20202020202020202No Hit
CCGCTGAGCTGCGACGAGCCCATTCTCAGCAACAAGTCTGGAGACAATCG30.15151515151515152No Hit
GCAGTGGCGCGGCCGCCGAGCCCCTGGACGAGGAGGAGGACGCGGACGAC30.15151515151515152No Hit
GTTGAGACCCCACGAGGCCTCCGGACCTTCAAGACTGTATTTGCTGAGCA30.15151515151515152No Hit
TGCGAGGCCTGCGGCCGAGAGCACTGCCCGTCGCCGCTGCGTGACCTCAG30.15151515151515152No Hit
TCCTTGTACCTTGACCTGCCGCCGAGTGCACCTGAAAGAGAAGGGCAAGC30.15151515151515152No Hit
GTGCTGCCGGCGAGAGCCAAGTACATCTTGACAACGCTCATGGCTGCGGA30.15151515151515152No Hit
CGGGGAGTGGAGTCCGGGGTCTGAGCGCGCGCTGCCAGGCATCTGCTTCT20.10101010101010101No Hit
AATACACACACACTTCTGGTGCTGCCAACATTTAATGTGTCTAATCACAA20.10101010101010101No Hit
CTTCTGGACCAGCGCATCCTTCCGCTGCAGTTCACTCCTTAGTTCCGAGA20.10101010101010101No Hit
GTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCGAAGTCGGGCGGCGG20.10101010101010101No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC20.10101010101010101No Hit
CTGGTGGCGAGCCCGAGTGTGATGGTTCATTGCTGTGCGCATTGCATTGG20.10101010101010101No Hit
GATGTGGAGACATCCAAGAAATGGGCTGCTGGCCAGAACAAGCAGCATTC20.10101010101010101No Hit
CCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATG20.10101010101010101No Hit
CAGCTGAGCTGAGGGTACAGCTTGCATTCTTGGTGTCCACAAGGGAGACA20.10101010101010101No Hit
GAGGCAACATGTCGTCGCCGACGATCCCTTCGCGCCTGTACTCCTTGCTG20.10101010101010101No Hit
GTATCAACGCAGAGTACATGGGGCCATTTCGTGACGCCGCGAGACCGAGA20.10101010101010101No Hit
CCACAAAGATGGTCACGCCAATGGCGACGGTCGCTCCGAACCATGAGGCT20.10101010101010101No Hit
GTAGGGGAGTAACTTCAGTGCTCAGTGCAGGGGTCTCAGACTGTCTCTTA20.10101010101010101No Hit
GTGTCCTGCTGCCCACTGCCAGGTGCTGCCGGCGAGAGCCAAGTACATCT20.10101010101010101No Hit
TTACCACTCCTTGTACCTTGACCTGCCGCCGAGTGCACCTGAAAGAGAAG20.10101010101010101No Hit
GCTTACAGCGACCACGCTGGTGGAGAGAAGTAGGGACAGGATTTGCGCTC20.10101010101010101No Hit
GTTCACAGGAACCTGCTGCTCCAGCCGCTCTCGGGCCCAGTCGAGCTTCT20.10101010101010101No Hit
CCCTTCCCGTGGGTTGGGATGTCTCTCGCTACTGATGGTGGCAGCAGTGG20.10101010101010101No Hit
GATCTAAGGTGAACAAGAAAGAAGTCGTGGAGGCTGTGACCATTGTGGAA20.10101010101010101No Hit
GTCCCATGTATCCTGGGACAAAATGGAATCTCGGATGTTGTGAAGGTGAC20.10101010101010101No Hit
GTATGCCCGGCGCCGGAGCTACCTGTTTTTAGGAGGTATCTTGATGTCAG20.10101010101010101No Hit
ATCTGACACTGTATCAGGAGGCTCATGGAGGACGTCATGGGGAGGCGAGG20.10101010101010101No Hit
GTTGGAAGGCTAATTAAGCCTCCAACTTGCTCAAATAGAATTACAAAAAG20.10101010101010101No Hit
GAGCTGCGACGAGCCCATTCTCAGCAACAAGTCTGGAGACAATCGCGGCA20.10101010101010101No Hit
GCGCTGCTCGCCGAGGGCATAACCTGGGTCCTGGTTTACAGGACAGACAA20.10101010101010101No Hit
GCCTGTCACTGCCGTCTTGACCAGTTTGTCAATTTGATACTTCAGTTTTT20.10101010101010101No Hit
GTCCGCGCACGCTGCCACTGGGTGCTCCAGCTGCTGGCCCTACTCTGTGC20.10101010101010101No Hit
TCGCTGGCCGCCGTGTCCTCGGATTTATTCCAACAGTCAGTTAAAACATG20.10101010101010101No Hit
GGATGTGGCTGCCTCCGAGTTCTACAGGTCTGGCAAGTATGACCTGGACT20.10101010101010101No Hit
CTTCCACCTCTGCCTCCACCTCTGCCTCCGAAACCTCCTCTGCCTCCACC20.10101010101010101No Hit
GTTGATGATAGCATTCACCAGGGCCTGCAGAGGGTTCTCACCAGTGAGCA20.10101010101010101No Hit
GTCCAGCATTTGCCATGGACAAGATGCCAGGACCTGTATGCTTTAGGATG20.10101010101010101No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)