FastQCFastQC Report
Thu 2 Feb 2017
SRR4062220_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062220_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561835
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27790.17793172774332758No Hit
TATCAACGCAGAGTACTTTTTTTTT18240.11678570399562055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6900.015.823991
TAGGTCT508.710209E-515.200774
TCGCGTA751.47733535E-512.6677139
TACTAAT604.0991534E-412.664062
GTATTAC604.112471E-412.6591911
AACCGTG701.0900642E-412.2152957
GTATCAA19750.012.2105121
CGCGTAA802.863953E-511.87598110
TCGCGCA658.0178905E-411.69327459
TAGCACC1104.964386E-711.227844
TTTTCGT1201.2741111E-711.08389416
TTTCGTC1201.274675E-711.08353917
CTTACGG600.005876267311.0831833
CTACACT1303.2625394E-810.9620934
CGTTATT700.001493970910.8549082
TACACTG2900.010.8112385
GTCTTAG1605.493348E-1010.6811921
GTCTTAC803.791317E-410.6811921
GTATTGG2150.010.5983931
CGACCCG1355.6781573E-810.5564285
Produced by FastQC (version 0.11.5)