##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062220_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1561835
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.268883076637415	32.0	32.0	32.0	32.0	32.0
2	30.89656589844638	32.0	32.0	32.0	32.0	32.0
3	30.9306956240576	32.0	32.0	32.0	32.0	32.0
4	30.93545797091242	32.0	32.0	32.0	32.0	32.0
5	30.835129831256182	32.0	32.0	32.0	32.0	32.0
6	34.491281729504074	36.0	36.0	36.0	32.0	36.0
7	34.44660607554575	36.0	36.0	36.0	32.0	36.0
8	34.405700346067285	36.0	36.0	36.0	32.0	36.0
9	34.53177576376506	36.0	36.0	36.0	32.0	36.0
10	34.2453127250958	36.0	36.0	36.0	32.0	36.0
11	34.49676566346637	36.0	36.0	36.0	32.0	36.0
12	34.35526800206168	36.0	36.0	36.0	32.0	36.0
13	34.428451789081436	36.0	36.0	36.0	32.0	36.0
14	34.34506718059206	36.0	36.0	36.0	32.0	36.0
15	34.29167997899906	36.0	36.0	36.0	32.0	36.0
16	34.27965886281201	36.0	36.0	36.0	32.0	36.0
17	34.23098086545634	36.0	36.0	36.0	32.0	36.0
18	34.22801064132895	36.0	36.0	36.0	32.0	36.0
19	34.24117976610846	36.0	36.0	36.0	32.0	36.0
20	34.216433874256886	36.0	36.0	36.0	32.0	36.0
21	34.20157571062244	36.0	36.0	36.0	32.0	36.0
22	34.17391978025848	36.0	36.0	36.0	32.0	36.0
23	34.14345113280212	36.0	36.0	36.0	32.0	36.0
24	34.11754634772559	36.0	36.0	36.0	32.0	36.0
25	33.77008582852862	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	24.0
5	111.0
6	312.0
7	85.0
8	277.0
9	218.0
10	113.0
11	29.0
12	60.0
13	69.0
14	191.0
15	294.0
16	526.0
17	704.0
18	897.0
19	1101.0
20	1714.0
21	2352.0
22	3388.0
23	5034.0
24	7154.0
25	10471.0
26	14762.0
27	19996.0
28	27601.0
29	38200.0
30	52266.0
31	73270.0
32	107373.0
33	154856.0
34	337397.0
35	700990.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.16342802847193	17.252959529881505	11.821344214662519	25.762268226984048
2	17.382192985938328	18.56125554956545	37.10752871303865	26.94902275145758
3	20.07561690847857	21.918466916802192	27.310637589478944	30.6952785852403
4	13.803348831610082	14.900437285767435	33.81475962729857	37.48145425532392
5	15.808748884757055	35.26447830328075	32.660782325713065	16.265990486249127
6	35.514304897800194	33.634640814318224	16.058349734506017	14.792704553375566
7	31.619580279736592	29.231333267529045	19.18468486368898	19.96440158904538
8	28.815128027140513	32.490864818543024	18.228893260187785	20.465113894128677
9	26.90903123615802	14.73828203374446	18.467403281626698	39.88528344847082
10	17.662769958288933	25.87169456166198	29.36328118488657	27.102254295162513
11	37.46650774609554	21.386891873956923	21.010021869744545	20.136578510202995
12	24.8893749991986	23.102064786308784	27.591625453431075	24.416934761061537
13	30.04718311665	19.31744749596123	24.416108931456264	26.21926045593251
14	23.8950101824508	19.062881200894594	24.287372671289205	32.7547359453654
15	26.22156885247058	26.057699273678224	21.368503318102526	26.352228555748674
16	26.368505871206537	25.49535700961419	22.617895288803876	25.5182418303754
17	24.64265818589531	25.479367224365852	24.304051294905317	25.573923294833524
18	25.13918926021693	24.875617425995415	24.896385245617925	25.088808068169726
19	26.322707748785184	24.44796650947689	24.445723382737665	24.783602359000255
20	26.465619668828083	23.92248644256145	24.25374808222005	25.358145806390418
21	26.776760675303635	23.52315780839054	24.330951074836072	25.369130441469757
22	26.594715782470672	23.596954553524448	24.312169159566757	25.496160504438127
23	25.36202770115053	23.837214890569882	24.771992554074274	26.028764854205317
24	25.584991607958692	24.27539738029886	24.531804843223597	25.607806168518856
25	25.52601510196679	23.95884800561886	24.396667903482243	26.118468988932104
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	128.0
1	128.0
2	266.0
3	404.0
4	404.0
5	404.0
6	853.5
7	1303.0
8	1303.0
9	1303.0
10	1533.5
11	1764.0
12	1764.0
13	1764.0
14	2403.0
15	3042.0
16	3042.0
17	3042.0
18	4952.5
19	6863.0
20	6863.0
21	6863.0
22	10809.5
23	14756.0
24	14756.0
25	14756.0
26	22673.5
27	30591.0
28	30591.0
29	30591.0
30	40433.0
31	50275.0
32	50275.0
33	50275.0
34	63735.0
35	77195.0
36	77195.0
37	77195.0
38	94923.0
39	112651.0
40	112651.0
41	112651.0
42	134188.5
43	155726.0
44	155726.0
45	155726.0
46	178445.0
47	201164.0
48	201164.0
49	201164.0
50	211978.0
51	222792.0
52	222792.0
53	222792.0
54	216105.5
55	209419.0
56	209419.0
57	209419.0
58	192598.5
59	175778.0
60	175778.0
61	175778.0
62	153612.5
63	131447.0
64	131447.0
65	131447.0
66	107760.5
67	84074.0
68	84074.0
69	84074.0
70	63763.5
71	43453.0
72	43453.0
73	43453.0
74	32344.0
75	21235.0
76	21235.0
77	21235.0
78	16102.0
79	10969.0
80	10969.0
81	10969.0
82	7438.0
83	3907.0
84	3907.0
85	3907.0
86	2781.0
87	1655.0
88	1655.0
89	1655.0
90	1098.5
91	542.0
92	542.0
93	542.0
94	341.5
95	141.0
96	141.0
97	141.0
98	351.0
99	561.0
100	561.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010244359999615838
2	6.402724999759899E-5
3	9.604087499639846E-4
4	0.00998825099962544
5	0.033038060998761075
6	0.06217045974766861
7	0.10583704424603112
8	0.1061571804960191
9	0.11627348599563975
10	0.1317040532450611
11	0.1374024784948474
12	0.13196016224505147
13	0.12517327374530599
14	0.11512099549568296
15	0.1317680804950587
16	0.11838638524556051
17	0.12254815649540446
18	0.11070311524584862
19	0.09687322924636725
20	0.09200715824654973
21	0.09034244974661215
22	0.10269970899614876
23	0.08003406249699872
24	0.09143091299657134
25	0.0881014959966962
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1561835.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.4452718245768
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.84210769026241	36.95936758576069
2	15.21784527343954	15.65772373352897
3	5.557747570736658	8.577595035267862
4	2.493954225631602	5.132086122226788
5	1.3708633281617044	3.5262218275811454
6	0.8328919013098959	2.570901015802571
7	0.5423309628375959	1.95302546614452
8	0.37131170324264035	1.5281785203971383
9	0.25924494720036556	1.2003234100083782
>10	1.3604360641228317	12.423777278660411
>50	0.08937955724219201	3.1501534601262966
>100	0.058011162448590704	5.66905624080681
>500	0.0033755342202778957	1.2052319288452886
>1k	5.000791437448734E-4	0.44635837484317753
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2779	0.17793172774332758	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1824	0.11678570399562055	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.402724999759897E-5
2	0.0	0.0	0.0	0.0	6.402724999759897E-5
3	0.0	0.0	0.0	0.0	6.402724999759897E-5
4	0.0	0.0	0.0	0.0	6.402724999759897E-5
5	0.0	0.0	0.0	0.0	1.2805449999519795E-4
6	0.0	0.0	0.0	0.0	1.9208174999279693E-4
7	0.0	6.402724999759897E-5	0.0	0.0	1.9208174999279693E-4
8	0.0	6.402724999759897E-5	0.0	0.0	1.9208174999279693E-4
9	0.0	6.402724999759897E-5	0.0	6.402724999759897E-5	1.9208174999279693E-4
10	0.0	6.402724999759897E-5	0.0	6.402724999759897E-5	1.9208174999279693E-4
11	0.0	6.402724999759897E-5	0.0	6.402724999759897E-5	1.9208174999279693E-4
12	0.0	6.402724999759897E-5	0.0	6.402724999759897E-5	3.8416349998559386E-4
13	0.0	6.402724999759897E-5	0.0	1.2805449999519795E-4	5.762452499783908E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	690	0.0	15.82399	1
TAGGTCT	50	8.710209E-5	15.20077	4
TCGCGTA	75	1.47733535E-5	12.667713	9
TACTAAT	60	4.0991534E-4	12.66406	2
GTATTAC	60	4.112471E-4	12.659191	1
AACCGTG	70	1.0900642E-4	12.215295	7
GTATCAA	1975	0.0	12.210512	1
CGCGTAA	80	2.863953E-5	11.875981	10
TCGCGCA	65	8.0178905E-4	11.6932745	9
TAGCACC	110	4.964386E-7	11.22784	4
TTTTCGT	120	1.2741111E-7	11.083894	16
TTTCGTC	120	1.274675E-7	11.083539	17
CTTACGG	60	0.0058762673	11.083183	3
CTACACT	130	3.2625394E-8	10.962093	4
CGTTATT	70	0.0014939709	10.854908	2
TACACTG	290	0.0	10.811238	5
GTCTTAG	160	5.493348E-10	10.681192	1
GTCTTAC	80	3.791317E-4	10.681192	1
GTATTGG	215	0.0	10.598393	1
CGACCCG	135	5.6781573E-8	10.556428	5
>>END_MODULE