FastQCFastQC Report
Thu 2 Feb 2017
SRR4062220_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062220_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561835
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9800.028.7460291
GTATCAA20750.025.6679551
CCTTATA1203.1620402E-718.3341062
TAGGACG1203.1641684E-718.3329324
ATCAACG31900.016.2065433
TCAACGC32700.015.877334
TACACCG1958.931238E-1015.7945275
GTGTAAG2850.015.4446871
CTATTAG1158.192359E-515.3103881
TATCAAC35400.015.2887822
GTGTAGG2601.8189894E-1215.2367771
CTAGTAC1603.826426E-715.124673
CAACGCA34400.015.0926935
TATGGGT1756.4141204E-815.0853854
AACGCAG35050.014.9383336
ATACCGT3400.014.8820296
TACTAGA1809.051473E-814.6672872
CGCAATA1951.5157639E-814.66728536
TACACTA1809.058567E-814.66634755
CTATTCC3300.014.6663474
Produced by FastQC (version 0.11.5)