##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062220_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1561835
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.902328991218663	32.0	32.0	32.0	32.0	32.0
2	31.313118863388258	32.0	32.0	32.0	32.0	32.0
3	31.48295754673189	32.0	32.0	32.0	32.0	32.0
4	31.59120649748533	32.0	32.0	32.0	32.0	32.0
5	31.510474537963358	32.0	32.0	32.0	32.0	32.0
6	35.19600277878265	36.0	36.0	36.0	36.0	36.0
7	35.20406829146485	36.0	36.0	36.0	36.0	36.0
8	35.13928231855478	36.0	36.0	36.0	36.0	36.0
9	35.2614008522027	36.0	36.0	36.0	36.0	36.0
10	35.106063700711026	36.0	36.0	36.0	36.0	36.0
11	35.27338675340225	36.0	36.0	36.0	36.0	36.0
12	35.1840219997631	36.0	36.0	36.0	36.0	36.0
13	35.225911828074025	36.0	36.0	36.0	36.0	36.0
14	35.188056356785445	36.0	36.0	36.0	36.0	36.0
15	35.14572730153954	36.0	36.0	36.0	36.0	36.0
16	35.16240768070891	36.0	36.0	36.0	36.0	36.0
17	35.144681096274574	36.0	36.0	36.0	36.0	36.0
18	35.14371236398211	36.0	36.0	36.0	36.0	36.0
19	35.14056606491723	36.0	36.0	36.0	36.0	36.0
20	35.13540546856742	36.0	36.0	36.0	36.0	36.0
21	35.129212112675155	36.0	36.0	36.0	36.0	36.0
22	35.108477528035934	36.0	36.0	36.0	36.0	36.0
23	35.07871830250955	36.0	36.0	36.0	36.0	36.0
24	35.063597627150116	36.0	36.0	36.0	36.0	36.0
25	35.045879366258276	36.0	36.0	36.0	36.0	36.0
26	35.003553512374864	36.0	36.0	36.0	36.0	36.0
27	34.99388283653523	36.0	36.0	36.0	36.0	36.0
28	34.96390911972135	36.0	36.0	36.0	36.0	36.0
29	34.95000240102188	36.0	36.0	36.0	36.0	36.0
30	34.92887725015767	36.0	36.0	36.0	32.0	36.0
31	34.92639683449276	36.0	36.0	36.0	32.0	36.0
32	34.897017290558864	36.0	36.0	36.0	32.0	36.0
33	34.87495990293469	36.0	36.0	36.0	32.0	36.0
34	34.865996087935024	36.0	36.0	36.0	32.0	36.0
35	34.85351653663799	36.0	36.0	36.0	32.0	36.0
36	34.82209196233917	36.0	36.0	36.0	32.0	36.0
37	34.824641527434075	36.0	36.0	36.0	32.0	36.0
38	34.81008237105712	36.0	36.0	36.0	32.0	36.0
39	34.78416029862309	36.0	36.0	36.0	32.0	36.0
40	34.771596231356064	36.0	36.0	36.0	32.0	36.0
41	34.74778193599196	36.0	36.0	36.0	32.0	36.0
42	34.71679274699312	36.0	36.0	36.0	32.0	36.0
43	34.71033111692336	36.0	36.0	36.0	32.0	36.0
44	34.666805392375	36.0	36.0	36.0	32.0	36.0
45	34.63891192091354	36.0	36.0	36.0	32.0	36.0
46	34.629732974353885	36.0	36.0	36.0	32.0	36.0
47	34.60207896480742	36.0	36.0	36.0	32.0	36.0
48	34.574277692585966	36.0	36.0	36.0	32.0	36.0
49	34.562861633911396	36.0	36.0	36.0	32.0	36.0
50	34.11254453895578	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	14.0
21	42.0
22	90.0
23	237.0
24	701.0
25	1707.0
26	3636.0
27	6572.0
28	11776.0
29	19251.0
30	29704.0
31	45046.0
32	71584.0
33	126415.0
34	291159.0
35	953896.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.49412236396638	18.10436150439814	12.372049173083155	26.029466958552323
2	16.895625944036823	19.06853539269691	36.6607396574543	27.375099005811972
3	19.44626566749817	22.389860112995507	27.620768430919583	30.54310578858674
4	13.592344901990286	15.274212704927217	34.005192609974806	37.12824978310769
5	15.501189306168705	35.6667637746625	32.426088543284024	16.405958375884776
6	35.72716894465534	33.81806354208618	15.83173851716351	14.623028996094966
7	31.639769886063508	29.23817176590357	19.228087474028946	19.893970874003976
8	28.848694004168173	32.48595402203178	18.17010119506862	20.495250778731428
9	27.061437347735197	14.512352457205788	18.270303841314863	40.15590635374415
10	17.54873920579217	25.998170098851798	29.44040752116263	27.0126831741934
11	37.800023690082504	21.171250484206077	20.95304561621426	20.07568020949716
12	24.878492286317055	23.069146228634906	27.59785764821508	24.454503836832956
13	30.226752505866493	19.191591941530316	24.378439463835807	26.203216088767377
14	23.98243092260066	18.904813888791068	24.298021237838825	32.81473395076945
15	26.301497917513693	26.046413353523263	21.30295453745114	26.349134191511908
16	26.47008919681885	25.490467254055666	22.55826029387378	25.4811832552517
17	24.752358603821786	25.458387089545308	24.22157270134169	25.56768160529121
18	25.26252840416153	24.869656191995166	24.95564500896704	24.91217039487627
19	26.43755582375859	24.39867207483505	24.467565395832466	24.696206705573893
20	26.700669600839017	23.75501496325455	24.237142933976	25.30717250193043
21	26.931846193740057	23.47975298286951	24.28451148808933	25.303889335301104
22	26.693216632999007	23.525532466617793	24.33470885208745	25.446542048295758
23	25.489648707865243	23.78748317682929	24.739889130342917	25.98297898496255
24	25.687613449736364	24.23520071199214	24.52300688621892	25.55417895205258
25	25.691559655583955	23.753835239641653	24.52562119357268	26.02898391120172
26	25.333023439856017	24.401678290873445	24.784755289798422	25.48054297947212
27	25.910903178260874	24.08804036805566	24.332113182685173	25.668943270998295
28	25.802742087190257	23.90493551363392	24.608417322799355	25.68390507637647
29	25.523185346392395	24.26600385317964	24.71093149241241	25.49987930801555
30	25.621569291586127	24.306046882693384	24.6648606459288	25.407523179791692
31	25.866133615396336	23.85041611549558	24.216718123529766	26.066732145578314
32	25.717992010608132	24.10128945634615	24.082977498701837	26.097741034343887
33	25.655848808225727	23.752121708430682	24.522115388924924	26.069914094418667
34	26.044021690065634	23.93123155598497	24.565939374614633	25.458807379334765
35	26.000619784970468	23.73251412443848	24.73774388026369	25.52912221032736
36	25.593967073219925	24.030991869148288	24.423928611903094	25.95111244572869
37	26.171337760692122	23.933348870921062	24.20924776732548	25.686065601061337
38	25.786526745783007	23.874801115354696	24.50694215458099	25.83172998428131
39	26.298886106520662	23.68347420539768	24.19550536906005	25.8221343190216
40	26.126929063478617	24.045720637225617	24.2554741768166	25.571876122479164
41	25.507806297284702	24.439818775066975	24.62261879760484	25.42975613004349
42	26.215925309572004	24.436514608904226	24.043302622117583	25.304257459406195
43	26.05655897674943	23.762364192376843	24.28081507624692	25.900261754626808
44	25.697987419450673	24.04262839229185	24.348754238764073	25.910629949493398
45	25.90957490743059	24.06727873781615	24.242397078757154	25.78074927599611
46	25.92158381861896	23.96031489408021	24.129735336583867	25.98836595071696
47	25.60379915982582	24.238276010216293	24.499637922192008	25.65828690776588
48	26.003255289204656	24.425618178395485	23.860744823488282	25.710381708911576
49	25.920606696636955	24.252498288799433	24.05695080386408	25.769944210699535
50	25.804311816934423	24.493709505884553	24.113891590541943	25.588087086639078
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	40.0
1	38.5
2	37.0
3	114.5
4	192.0
5	199.0
6	206.0
7	175.0
8	144.0
9	203.0
10	262.0
11	460.0
12	658.0
13	1227.0
14	1796.0
15	2227.0
16	2658.0
17	2517.5
18	2377.0
19	2238.5
20	2100.0
21	2400.0
22	2700.0
23	3258.5
24	3817.0
25	4856.5
26	5896.0
27	7190.0
28	8484.0
29	10368.5
30	12253.0
31	14474.5
32	16696.0
33	19563.5
34	22431.0
35	26647.0
36	30863.0
37	37355.0
38	43847.0
39	49737.5
40	55628.0
41	63112.0
42	70596.0
43	74994.0
44	79392.0
45	87398.0
46	95404.0
47	102599.5
48	109795.0
49	117605.5
50	125416.0
51	127370.0
52	129324.0
53	130358.5
54	131393.0
55	130301.5
56	129210.0
57	124470.5
58	119731.0
59	110704.5
60	101678.0
61	91107.0
62	80536.0
63	69669.5
64	58803.0
65	49765.5
66	40728.0
67	34306.0
68	27884.0
69	23576.0
70	19268.0
71	15113.5
72	10959.0
73	9337.0
74	7715.0
75	5987.0
76	4259.0
77	3555.5
78	2852.0
79	2262.5
80	1673.0
81	1344.0
82	1015.0
83	807.5
84	600.0
85	442.5
86	285.0
87	206.5
88	128.0
89	87.5
90	47.0
91	31.5
92	16.0
93	14.5
94	13.0
95	16.0
96	19.0
97	14.0
98	9.0
99	5.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0315654342488163
2	0.0037135804998607407
3	1.9208174999279693E-4
4	0.0
5	0.0
6	2.5610899999039594E-4
7	0.0
8	0.0
9	0.0
10	1.2805449999519797E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.122179999807919E-4
17	0.0
18	2.5610899999039594E-4
19	0.0
20	5.762452499783908E-4
21	0.0
22	0.0
23	6.402724999759899E-5
24	0.0012805449999519797
25	1.9208174999279693E-4
26	5.122179999807919E-4
27	3.8416349998559386E-4
28	0.0020488719999231676
29	5.122179999807919E-4
30	0.0014085994999471776
31	5.762452499783908E-4
32	8.963814999663857E-4
33	2.5610899999039594E-4
34	8.963814999663857E-4
35	1.9208174999279693E-4
36	3.8416349998559386E-4
37	0.001984844749925568
38	0.0
39	0.0010244359999615838
40	1.2805449999519797E-4
41	0.0012165177499543806
42	0.005250234499803116
43	0.0042898257498391315
44	0.0037776077498583396
45	0.0021769264999183654
46	0.0025610899999039594
47	0.0010244359999615838
48	0.004417880249834329
49	0.004225798499841532
50	0.0021769264999183654
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1561835.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.86027861596643
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.35822973211185	37.72015905181572
2	15.655231740315854	16.55079823181234
3	5.68224520366395	9.010951938897458
4	2.5794088058608575	5.453930725691287
5	1.3483215841615848	3.5636327301351303
6	0.8150879236575602	2.5851464844628924
7	0.5178613256560873	1.9162005771030153
8	0.35733093477064665	1.5110890216064086
9	0.26735874421204536	1.2719391938517257
>10	1.2923135829159624	12.051572942473712
>50	0.07915139418423396	2.9055827230663667
>100	0.04465306991835072	4.375007707435308
>500	0.0026839603722395833	1.0126642935866956
>1k	1.2199819873816287E-4	0.07132437806195538
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2805449999519795E-4
2	0.0	0.0	0.0	0.0	1.9208174999279693E-4
3	0.0	0.0	0.0	0.0	1.9208174999279693E-4
4	0.0	0.0	0.0	0.0	1.9208174999279693E-4
5	0.0	0.0	0.0	0.0	1.9208174999279693E-4
6	0.0	0.0	0.0	0.0	2.561089999903959E-4
7	0.0	0.0	0.0	0.0	2.561089999903959E-4
8	0.0	0.0	0.0	0.0	2.561089999903959E-4
9	0.0	0.0	0.0	0.0	2.561089999903959E-4
10	0.0	0.0	0.0	0.0	3.8416349998559386E-4
11	0.0	0.0	0.0	0.0	3.8416349998559386E-4
12	0.0	0.0	0.0	0.0	5.122179999807918E-4
13	0.0	0.0	0.0	0.0	6.402724999759898E-4
14	0.0	0.0	0.0	0.0	6.402724999759898E-4
15	0.0	0.0	0.0	6.402724999759897E-5	0.0010244359999615836
16	0.0	0.0	0.0	1.2805449999519795E-4	0.0010244359999615836
17	0.0	0.0	0.0	1.2805449999519795E-4	0.0010244359999615836
18	0.0	0.0	0.0	1.2805449999519795E-4	0.0012165177499543806
19	0.0	0.0	0.0	1.2805449999519795E-4	0.0012805449999519795
20	0.0	0.0	0.0	3.201362499879949E-4	0.0014726267499447765
21	0.0	0.0	0.0	3.8416349998559386E-4	0.0015366539999423754
22	0.0	0.0	0.0	3.8416349998559386E-4	0.0015366539999423754
23	0.0	0.0	0.0	6.402724999759898E-4	0.0015366539999423754
24	0.0	0.0	0.0	0.0012165177499543806	0.0017287357499351725
25	0.0	0.0	0.0	0.0014085994999471776	0.0017287357499351725
26	0.0	0.0	0.0	0.001984844749925568	0.0017287357499351725
27	0.0	0.0	0.0	0.002369008249911162	0.0017927629999327714
28	0.0	0.0	0.0	0.002881226249891954	0.0017927629999327714
29	0.0	0.0	0.0	0.0037776077498583396	0.0017927629999327714
30	0.0	0.0	0.0	0.006210643249767101	0.0017927629999327714
31	0.0	0.0	0.0	0.010948659749589425	0.0017927629999327714
32	0.0	0.0	0.0	0.017287357499351723	0.0017927629999327714
33	0.0	0.0	0.0	0.024586463999078007	0.0017927629999327714
34	0.0	0.0	0.0	0.0313733524988235	0.0019208174999279693
35	0.0	0.0	0.0	0.03931273149852577	0.0019208174999279693
36	0.0	0.0	0.0	0.0497491732481344	0.0019208174999279693
37	0.0	0.0	0.0	0.06793291224745251	0.0019208174999279693
38	0.0	0.0	0.0	0.08861371399667699	0.0019208174999279693
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	980	0.0	28.746029	1
GTATCAA	2075	0.0	25.667955	1
CCTTATA	120	3.1620402E-7	18.334106	2
TAGGACG	120	3.1641684E-7	18.332932	4
ATCAACG	3190	0.0	16.206543	3
TCAACGC	3270	0.0	15.87733	4
TACACCG	195	8.931238E-10	15.794527	5
GTGTAAG	285	0.0	15.444687	1
CTATTAG	115	8.192359E-5	15.310388	1
TATCAAC	3540	0.0	15.288782	2
GTGTAGG	260	1.8189894E-12	15.236777	1
CTAGTAC	160	3.826426E-7	15.12467	3
CAACGCA	3440	0.0	15.092693	5
TATGGGT	175	6.4141204E-8	15.085385	4
AACGCAG	3505	0.0	14.938333	6
ATACCGT	340	0.0	14.882029	6
TACTAGA	180	9.051473E-8	14.667287	2
CGCAATA	195	1.5157639E-8	14.667285	36
TACACTA	180	9.058567E-8	14.6663475	5
CTATTCC	330	0.0	14.666347	4
>>END_MODULE