FastQCFastQC Report
Thu 2 Feb 2017
SRR4062219_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062219_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228027
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12030.5275691036587773No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA10560.46310305358575954No Hit
GATATACACTGTTCTACAAATCCCG9970.43722892464488855No Hit
GTCCTACAGTGGACATTTCTAAATT8900.39030465690466476No Hit
GTCCTACAGTGTGCATTTCTCATTT8640.3789024983883488No Hit
CTGTAGGACGTGGAATATGGCAAGA8470.37144724089691133No Hit
TATCAACGCAGAGTACTTTTTTTTT8170.35829090414731585No Hit
GTGTATATCAATGAGTTACAATGAG7580.33241677520644486No Hit
TTGTAGAACAGTGTATATCAATGAG7150.3135593591986914No Hit
GTCCTAAAGTGTGTATTTCTCATTT7090.3109280918487723No Hit
CCATAGGGTCTTCTCGTCTTATTAT6990.3065426462655738No Hit
GTGTATATCAATGAGTTACAATGAA6210.27233617071662564No Hit
GTTCTACAGTGTGGTTTTTATCATT5870.2574256557337508No Hit
CTTTAGGACGTGAAATATGGCGAGG5830.2556714775004714No Hit
CTGTAGGACCTGGAATATGGCGAGA5810.2547943883838317No Hit
GGTATCAACGCAGAGTACTTTTTTT4970.2179566454849645No Hit
ACGCAGAGTACTTTTTTTTTTTTTT4670.20480030873536909No Hit
GTTCTACAAATCCCGTTTCCAACGA4430.19427523933569268No Hit
GTACTTTTTTTTTTTTTTTTTTTTT4290.18813561551921482No Hit
GTATTAGAGGCACTGCCTGCCCAGT3880.17015528862810106No Hit
GTACATGGGGTGGTATCAACGCAAA3700.1622614865783438No Hit
GAGTACTTTTTTTTTTTTTTTTTTT3550.15568331820354608No Hit
GATATACACTGTTCTACAATGCCGG3500.15349059541194685No Hit
CTCTAATACTTGTAATGCTAGAGGT3400.14910514982874834No Hit
CCATTGGGATGTCCTGATCCAACAT3120.1368259021957926No Hit
CTTCTACACCATTGGGATGTCCTGA3080.1350717239625132No Hit
CTGTAGAACATATTAGATGAGTGAG3010.13200191205427425No Hit
CTGTTAGTATGAGTAACAAGAATTC3010.13200191205427425No Hit
GTTATATAATTTAAGCTCCATAGGG3000.13156336749595443No Hit
ACCTATAACTTCTCTGTTAACCCAA3000.13156336749595443No Hit
CTGAAGGACCTGGAATATGGCGAGA2930.1284935555877155No Hit
GTATCAACGCAGAGTACATGGGGTG2920.12805501102939565No Hit
GTCAGGATACCGCGGCCGTTAAACT2880.12630083279611626No Hit
CCTATAACTTCTCTGTTAACCCAAC2800.12279247632955746No Hit
ATTTAGAAATGTCCACTGTAGGACG2800.12279247632955746No Hit
ATGTTCTACAGTGTGGTTTTTATCA2800.12279247632955746No Hit
TTTCTAAATTTTCCACCTTTTTCAG2730.11972266442131851No Hit
ATCTATAACTTTATAGATGCAACAC2630.11533721883812005No Hit
ATAAATAATCCACCTATAACTTCTC2610.11446012972148034No Hit
GACTATAGGCAATAATCACACTATA2590.11358304060484065No Hit
ATCCTGACCGTGCAAAGGTAGCATA2570.11270595148820095No Hit
CACTATAAATAATCCACCTATAACT2530.11095177325492157No Hit
GCCTAAAGGAAAGATCCAAAAAGAT2510.11007468413828188No Hit
GTCCTTCAGTGTGCATTTCTCATTT2500.10963613957996202No Hit
GTTCATGCTAGTCCCTAATTAAGGA2480.10875905046332232No Hit
CCTCTAGCATTACAAGTATTAGAGG2450.10744341678836278No Hit
GGTCAGGATACCGCGGCCGTTAAAC2420.10612778311340322No Hit
TTGATATACACTGTTCTACAAATCC2370.10393506032180401No Hit
GAATATGGCAAGAAAACTGAAAATC2340.10261942664684445No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGAC350.002160056516.28853815
TCCAAAA350.002160056516.28853815
GATGCAA508.64921E-515.20263616
ATTTAGA851.6969352E-814.5255521
ACGGACC400.005254768814.2524716
CACACAG400.005262433614.2493415
CGGACCA400.005262433614.24934117
CCAACGA707.184788E-613.57378219
TAGGACC2750.013.4721054
CACCTTT852.6593807E-713.4140914
TTTAGAA852.666511E-713.4111452
CAACGGA500.001490755513.30230614
CCTTTTG500.001490755513.30230615
TTCCACC1009.8771125E-913.30230611
TTGCATA500.001490755513.30230619
TCAACGG500.001490755513.30230613
GATACTG500.001493225813.2993855
AAAGGAA500.001493225813.2993855
CTTGTAC500.001495699713.2964659
TTGTACC500.001495699713.29646510
Produced by FastQC (version 0.11.5)