##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062219_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 228027 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.148539427348517 32.0 32.0 32.0 32.0 32.0 2 30.82417433023282 32.0 32.0 32.0 32.0 32.0 3 30.875891889995483 32.0 32.0 32.0 32.0 32.0 4 30.858082595482113 32.0 32.0 32.0 32.0 32.0 5 30.881601740144806 32.0 32.0 32.0 32.0 32.0 6 34.49047700491609 36.0 36.0 36.0 32.0 36.0 7 34.38326601674363 36.0 36.0 36.0 32.0 36.0 8 34.33969222942897 36.0 36.0 36.0 32.0 36.0 9 34.349173562779846 36.0 36.0 36.0 32.0 36.0 10 34.17529503085161 36.0 36.0 36.0 32.0 36.0 11 34.43780341801629 36.0 36.0 36.0 32.0 36.0 12 34.262407521916266 36.0 36.0 36.0 32.0 36.0 13 34.324273002758446 36.0 36.0 36.0 32.0 36.0 14 34.28611962618462 36.0 36.0 36.0 32.0 36.0 15 34.25682046424327 36.0 36.0 36.0 32.0 36.0 16 34.262710117661506 36.0 36.0 36.0 32.0 36.0 17 34.21842150271679 36.0 36.0 36.0 32.0 36.0 18 34.245041157406796 36.0 36.0 36.0 32.0 36.0 19 34.116569529047 36.0 36.0 36.0 32.0 36.0 20 34.050362457077455 36.0 36.0 36.0 32.0 36.0 21 34.00105689238555 36.0 36.0 36.0 32.0 36.0 22 33.962451814916655 36.0 36.0 36.0 32.0 36.0 23 34.002543558438255 36.0 36.0 36.0 32.0 36.0 24 33.92095672880843 36.0 36.0 36.0 32.0 36.0 25 33.59622325426375 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 2.0 5 22.0 6 47.0 7 2.0 8 40.0 9 22.0 10 15.0 11 2.0 12 3.0 13 9.0 14 97.0 15 123.0 16 150.0 17 202.0 18 253.0 19 325.0 20 457.0 21 628.0 22 848.0 23 1104.0 24 1492.0 25 2008.0 26 2462.0 27 3146.0 28 4145.0 29 5273.0 30 7013.0 31 9551.0 32 13930.0 33 19948.0 34 46492.0 35 108215.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.00109637101195 21.37923473303366 12.36969630522969 24.2499725907247 2 12.581843378196442 21.96801255991615 45.40646502387875 20.04367903800866 3 18.953978932227027 26.897809898871184 30.887705787936465 23.26050538096533 4 9.98320123863452 18.905404990504092 41.03327733746212 30.07811643339927 5 10.062426792192921 41.45525056263079 36.85593580963996 11.626386835536332 6 27.481482131573404 42.459892578810646 18.513041494067263 11.54558379554869 7 24.699183922527514 34.17209181859287 21.857179857504708 19.271544401374907 8 22.306115371614947 41.92569895918842 19.959789818395716 15.80839585080091 9 25.236849268159904 15.6052717071586 21.30238561431544 37.85549341036605 10 14.456831484059066 29.726846017730203 34.56115777595314 21.255164722257593 11 33.00721041953928 23.432107005787657 26.910409878538243 16.65027269613483 12 22.53532257922883 26.855697714240556 33.73712449178514 16.871855214745477 13 28.774467188282095 23.554800354768744 27.421691825390553 20.249040631558614 14 18.711504269376825 26.216388260860025 27.98472243563009 27.087385034133067 15 21.904268445295312 35.0705406580282 23.844630523269856 19.18056037340663 16 19.478164663941733 29.322615059730524 31.62095595175899 19.57826432456876 17 17.405678809618937 30.992408642468572 30.587155721618025 21.014756826294462 18 17.344981233126276 29.95236945499243 34.67020786233236 18.032441449548937 19 22.608481911634318 27.23920886992705 28.117949663339566 22.034359555099066 20 21.60990168539326 30.75096558988764 29.712517556179773 17.926615168539325 21 21.43581370238205 27.444706336770118 27.798041513591333 23.321438447256497 22 20.922739244951714 31.726953467954345 28.41966637401229 18.93064091308165 23 21.131167902112328 30.649585478356663 29.53132063216196 18.68792598736905 24 22.179891764726538 29.4605353827517 30.034630020584892 18.324942831936866 25 20.854784131456636 30.436213775033245 30.785571019148815 17.923431074361304 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1169.0 1 1169.0 2 696.5 3 224.0 4 224.0 5 224.0 6 592.5 7 961.0 8 961.0 9 961.0 10 928.0 11 895.0 12 895.0 13 895.0 14 1107.5 15 1320.0 16 1320.0 17 1320.0 18 2211.0 19 3102.0 20 3102.0 21 3102.0 22 5438.0 23 7774.0 24 7774.0 25 7774.0 26 12513.5 27 17253.0 28 17253.0 29 17253.0 30 22616.5 31 27980.0 32 27980.0 33 27980.0 34 28477.5 35 28975.0 36 28975.0 37 28975.0 38 28660.5 39 28346.0 40 28346.0 41 28346.0 42 26922.5 43 25499.0 44 25499.0 45 25499.0 46 26835.0 47 28171.0 48 28171.0 49 28171.0 50 25146.5 51 22122.0 52 22122.0 53 22122.0 54 18165.5 55 14209.0 56 14209.0 57 14209.0 58 11538.5 59 8868.0 60 8868.0 61 8868.0 62 7018.5 63 5169.0 64 5169.0 65 5169.0 66 4131.5 67 3094.0 68 3094.0 69 3094.0 70 2375.5 71 1657.0 72 1657.0 73 1657.0 74 1246.5 75 836.0 76 836.0 77 836.0 78 530.5 79 225.0 80 225.0 81 225.0 82 138.5 83 52.0 84 52.0 85 52.0 86 42.0 87 32.0 88 32.0 89 32.0 90 21.0 91 10.0 92 10.0 93 10.0 94 6.0 95 2.0 96 2.0 97 2.0 98 42.0 99 82.0 100 82.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 8.770891166396962E-4 2 0.0 3 4.385445583198481E-4 4 0.014910514982874835 5 0.035083564665587846 6 0.06095769360645888 7 0.10086524841356508 8 0.09823398106364598 9 0.10744341678836278 10 0.12279247632955746 11 0.13200191205427425 12 0.11796848618803914 13 0.11972266442131851 14 0.10612778311340322 15 0.12542374367947656 16 0.11139031781324141 17 0.11752994162971928 18 0.10174233753020476 19 0.08814745622228946 20 0.0820078324058116 21 0.08683182254732992 22 0.09954961473860552 23 0.07630675314765357 24 0.08332346608077114 25 0.07893802049757266 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 228027.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.785674503457926 #Duplication Level Percentage of deduplicated Percentage of total 1 67.03839413732675 23.99014151832897 2 15.418928689599392 11.035535265560657 3 6.439872060391416 6.913654961912405 4 3.215646867072707 4.602963684125125 5 1.7830663839903924 3.190411661776895 6 1.1666523694562567 2.5049665171229725 7 0.7438634330461636 1.8633758283010344 8 0.5343071776081175 1.52964341941963 9 0.43381821301209544 1.397202962807036 >10 2.7303586965846005 18.888991215952498 >50 0.30881974485606795 7.666635968547585 >100 0.16789009938603694 11.185956049064364 >500 0.015931177314003505 4.239848789836291 >1k 0.0024509503560005393 0.9906721572445369 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1203 0.5275691036587773 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 1056 0.46310305358575954 No Hit GATATACACTGTTCTACAAATCCCG 997 0.43722892464488855 No Hit GTCCTACAGTGGACATTTCTAAATT 890 0.39030465690466476 No Hit GTCCTACAGTGTGCATTTCTCATTT 864 0.3789024983883488 No Hit CTGTAGGACGTGGAATATGGCAAGA 847 0.37144724089691133 No Hit TATCAACGCAGAGTACTTTTTTTTT 817 0.35829090414731585 No Hit GTGTATATCAATGAGTTACAATGAG 758 0.33241677520644486 No Hit TTGTAGAACAGTGTATATCAATGAG 715 0.3135593591986914 No Hit GTCCTAAAGTGTGTATTTCTCATTT 709 0.3109280918487723 No Hit CCATAGGGTCTTCTCGTCTTATTAT 699 0.3065426462655738 No Hit GTGTATATCAATGAGTTACAATGAA 621 0.27233617071662564 No Hit GTTCTACAGTGTGGTTTTTATCATT 587 0.2574256557337508 No Hit CTTTAGGACGTGAAATATGGCGAGG 583 0.2556714775004714 No Hit CTGTAGGACCTGGAATATGGCGAGA 581 0.2547943883838317 No Hit GGTATCAACGCAGAGTACTTTTTTT 497 0.2179566454849645 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 467 0.20480030873536909 No Hit GTTCTACAAATCCCGTTTCCAACGA 443 0.19427523933569268 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 429 0.18813561551921482 No Hit GTATTAGAGGCACTGCCTGCCCAGT 388 0.17015528862810106 No Hit GTACATGGGGTGGTATCAACGCAAA 370 0.1622614865783438 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 355 0.15568331820354608 No Hit GATATACACTGTTCTACAATGCCGG 350 0.15349059541194685 No Hit CTCTAATACTTGTAATGCTAGAGGT 340 0.14910514982874834 No Hit CCATTGGGATGTCCTGATCCAACAT 312 0.1368259021957926 No Hit CTTCTACACCATTGGGATGTCCTGA 308 0.1350717239625132 No Hit CTGTAGAACATATTAGATGAGTGAG 301 0.13200191205427425 No Hit CTGTTAGTATGAGTAACAAGAATTC 301 0.13200191205427425 No Hit GTTATATAATTTAAGCTCCATAGGG 300 0.13156336749595443 No Hit ACCTATAACTTCTCTGTTAACCCAA 300 0.13156336749595443 No Hit CTGAAGGACCTGGAATATGGCGAGA 293 0.1284935555877155 No Hit GTATCAACGCAGAGTACATGGGGTG 292 0.12805501102939565 No Hit GTCAGGATACCGCGGCCGTTAAACT 288 0.12630083279611626 No Hit CCTATAACTTCTCTGTTAACCCAAC 280 0.12279247632955746 No Hit ATTTAGAAATGTCCACTGTAGGACG 280 0.12279247632955746 No Hit ATGTTCTACAGTGTGGTTTTTATCA 280 0.12279247632955746 No Hit TTTCTAAATTTTCCACCTTTTTCAG 273 0.11972266442131851 No Hit ATCTATAACTTTATAGATGCAACAC 263 0.11533721883812005 No Hit ATAAATAATCCACCTATAACTTCTC 261 0.11446012972148034 No Hit GACTATAGGCAATAATCACACTATA 259 0.11358304060484065 No Hit ATCCTGACCGTGCAAAGGTAGCATA 257 0.11270595148820095 No Hit CACTATAAATAATCCACCTATAACT 253 0.11095177325492157 No Hit GCCTAAAGGAAAGATCCAAAAAGAT 251 0.11007468413828188 No Hit GTCCTTCAGTGTGCATTTCTCATTT 250 0.10963613957996202 No Hit GTTCATGCTAGTCCCTAATTAAGGA 248 0.10875905046332232 No Hit CCTCTAGCATTACAAGTATTAGAGG 245 0.10744341678836278 No Hit GGTCAGGATACCGCGGCCGTTAAAC 242 0.10612778311340322 No Hit TTGATATACACTGTTCTACAAATCC 237 0.10393506032180401 No Hit GAATATGGCAAGAAAACTGAAAATC 234 0.10261942664684445 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.770891166396962E-4 0.0 5 0.0 0.0 0.0 8.770891166396962E-4 0.0 6 0.0 0.0 0.0 8.770891166396962E-4 0.0 7 0.0 0.0 0.0 8.770891166396962E-4 0.0 8 0.0 0.0 0.0 0.0013156336749595443 0.0 9 0.0 0.0 0.0 0.0017541782332793924 0.0 10 0.0 0.0 0.0 0.0017541782332793924 0.0 11 0.0 0.0 0.0 0.0017541782332793924 0.0 12 0.0 0.0 0.0 0.0017541782332793924 0.0 13 0.0 0.0 0.0 0.0017541782332793924 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGGAC 35 0.0021600565 16.288538 15 TCCAAAA 35 0.0021600565 16.288538 15 GATGCAA 50 8.64921E-5 15.202636 16 ATTTAGA 85 1.6969352E-8 14.525552 1 ACGGACC 40 0.0052547688 14.25247 16 CACACAG 40 0.0052624336 14.249341 5 CGGACCA 40 0.0052624336 14.249341 17 CCAACGA 70 7.184788E-6 13.573782 19 TAGGACC 275 0.0 13.472105 4 CACCTTT 85 2.6593807E-7 13.41409 14 TTTAGAA 85 2.666511E-7 13.411145 2 CAACGGA 50 0.0014907555 13.302306 14 CCTTTTG 50 0.0014907555 13.302306 15 TTCCACC 100 9.8771125E-9 13.302306 11 TTGCATA 50 0.0014907555 13.302306 19 TCAACGG 50 0.0014907555 13.302306 13 GATACTG 50 0.0014932258 13.299385 5 AAAGGAA 50 0.0014932258 13.299385 5 CTTGTAC 50 0.0014956997 13.296465 9 TTGTACC 50 0.0014956997 13.296465 10 >>END_MODULE