##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062219_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	228027
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.046748849916895	32.0	32.0	32.0	32.0	32.0
2	31.419195972406776	32.0	32.0	32.0	32.0	32.0
3	31.57564674358738	32.0	32.0	32.0	32.0	32.0
4	31.679542334898937	32.0	32.0	32.0	32.0	32.0
5	31.66151815355199	32.0	32.0	32.0	32.0	32.0
6	35.296824498853205	36.0	36.0	36.0	36.0	36.0
7	35.30185899038272	36.0	36.0	36.0	36.0	36.0
8	35.27083634832717	36.0	36.0	36.0	36.0	36.0
9	35.322988067202566	36.0	36.0	36.0	36.0	36.0
10	35.232586491950514	36.0	36.0	36.0	36.0	36.0
11	35.32475978721818	36.0	36.0	36.0	36.0	36.0
12	35.27167396843356	36.0	36.0	36.0	36.0	36.0
13	35.29184263267069	36.0	36.0	36.0	36.0	36.0
14	35.25300951203147	36.0	36.0	36.0	36.0	36.0
15	35.24687427366057	36.0	36.0	36.0	36.0	36.0
16	35.26188126844628	36.0	36.0	36.0	36.0	36.0
17	35.2514877624141	36.0	36.0	36.0	36.0	36.0
18	35.243107175904605	36.0	36.0	36.0	36.0	36.0
19	35.180000613962385	36.0	36.0	36.0	36.0	36.0
20	35.12767786270924	36.0	36.0	36.0	36.0	36.0
21	35.10493055646919	36.0	36.0	36.0	36.0	36.0
22	35.07828020366009	36.0	36.0	36.0	36.0	36.0
23	35.090270011884556	36.0	36.0	36.0	36.0	36.0
24	35.05613808891052	36.0	36.0	36.0	36.0	36.0
25	35.02543119893697	36.0	36.0	36.0	36.0	36.0
26	35.02526016655922	36.0	36.0	36.0	36.0	36.0
27	34.98308095094002	36.0	36.0	36.0	36.0	36.0
28	34.895209777789475	36.0	36.0	36.0	36.0	36.0
29	34.83568173944314	36.0	36.0	36.0	32.0	36.0
30	34.78066632460191	36.0	36.0	36.0	32.0	36.0
31	34.79423050779074	36.0	36.0	36.0	32.0	36.0
32	34.75699368934381	36.0	36.0	36.0	32.0	36.0
33	34.7469203208392	36.0	36.0	36.0	32.0	36.0
34	34.70440342591009	36.0	36.0	36.0	32.0	36.0
35	34.698263802093614	36.0	36.0	36.0	32.0	36.0
36	34.59631096317541	36.0	36.0	36.0	32.0	36.0
37	34.521337385485054	36.0	36.0	36.0	32.0	36.0
38	34.46788757471703	36.0	36.0	36.0	32.0	36.0
39	34.268411196919665	36.0	36.0	36.0	32.0	36.0
40	34.36566722361825	36.0	36.0	36.0	32.0	36.0
41	34.326084191784304	36.0	36.0	36.0	32.0	36.0
42	34.30110907918799	36.0	36.0	36.0	32.0	36.0
43	34.32716739684336	36.0	36.0	36.0	32.0	36.0
44	34.17701851096581	36.0	36.0	36.0	32.0	36.0
45	34.12452472733492	36.0	36.0	36.0	32.0	36.0
46	33.89734110434291	36.0	36.0	36.0	32.0	36.0
47	33.894376543128665	36.0	36.0	36.0	32.0	36.0
48	33.785560481872764	36.0	36.0	36.0	32.0	36.0
49	33.69456248602139	36.0	36.0	36.0	27.0	36.0
50	33.39836949133217	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	0.0
21	12.0
22	22.0
23	53.0
24	148.0
25	347.0
26	840.0
27	1613.0
28	2690.0
29	3943.0
30	5750.0
31	7880.0
32	11360.0
33	18007.0
34	38324.0
35	137037.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.56335186021926	21.922994248915366	13.028334291116305	24.485319599749076
2	12.24787842905072	22.271780365326844	45.153608315242415	20.32673289038002
3	18.669800812188083	27.010516344627366	31.316165700402586	23.003517142781963
4	9.822082472689637	18.861801453336664	41.37931034482759	29.93680572914611
5	10.038723484499643	41.436321137409166	36.92852162243945	11.596433755651743
6	27.619974827542354	42.27525687747504	18.582887114245242	11.521881180737369
7	24.72119529704816	33.92142158604025	22.183337938051196	19.1740451788604
8	22.412258197494157	41.381064523060864	20.05858955299153	16.148087726453447
9	25.266306183039728	15.109614212352046	21.604897665627316	38.019181938980914
10	14.418906532998285	29.485543378634986	34.79368671253843	21.3018633758283
11	33.22238156007841	22.849487122139045	27.127050743990843	16.801080573791698
12	22.62407521916264	26.441167054778603	34.09420814201827	16.840549584040488
13	28.881667521828557	22.645125357961994	27.925640384691285	20.547566735518163
14	18.989417919807742	25.54653615580611	28.486977419340693	26.977068505045455
15	21.978099084757506	34.69545273147478	24.298438342827822	19.028009840939887
16	19.62635675912729	28.777984870080036	31.63293498519899	19.962723385593684
17	17.462405767738034	30.368772119091158	30.84196169751828	21.326860415652533
18	17.607125471983583	28.84351414525473	35.45325772825148	18.09610265451021
19	22.80607121086538	26.39380424248006	29.091291820705443	21.70883272594912
20	21.717165072557197	29.80524236165015	30.502528209378717	17.975064356413935
21	21.56586719993685	26.692014542137553	28.708880965850536	23.03323729207506
22	20.972516412530094	30.788459261403254	29.35617273393063	18.882851592136017
23	20.90366491687388	29.75480973744337	30.349914703083407	18.99161064259934
24	22.418791086864047	28.708507475123124	30.52542945229209	18.347271985720738
25	20.942256838006028	29.561411587224317	31.58354054563714	17.912791029132517
26	17.29627323199986	30.538184242147825	33.74615175462447	18.419390771227842
27	18.07417542659422	30.4292912681393	32.158033917036136	19.33849938823034
28	17.669115228593355	31.266308518802767	30.306764609143734	20.757811643460148
29	19.526196459191237	27.736189135497114	29.461423427927397	23.276190977384257
30	19.120355752026104	29.201750692909517	30.83710486615444	20.84078868890994
31	21.607813212644285	27.372557274672843	30.845437322387113	20.17419219029576
32	18.479033092717497	30.53423732381395	31.116188504819625	19.870541078648927
33	19.42717058581039	30.410567216019224	31.908203450483718	18.254058747686667
34	19.068690439122367	30.18248159176925	32.92869578945983	17.820132179648542
35	18.916180978568327	31.392335118209687	31.052463085511807	18.639020817710183
36	17.96059238598938	30.33588127721717	31.5870489021037	20.116477434689752
37	19.0697348051276	29.57096056942124	28.942948237223764	22.416356388227403
38	19.9827213444022	31.75895836896508	29.126375385371027	19.131944901261694
39	20.80025961951707	28.46999903519774	29.857560102795293	20.872181242489894
40	22.468391900959098	29.38336249654646	30.64987918097418	17.49836642152026
41	21.4602099797388	27.655226250098675	32.57536553490453	18.309198235258002
42	19.524068486422006	32.57359132692443	29.74571959862466	18.15662058802891
43	17.39593795090631	32.195971282339165	31.585041203791015	18.823049562963515
44	17.73822889621781	31.44691600589432	30.472422987860504	20.342432110027367
45	18.14386331143486	31.57326924594995	30.747910289358042	19.534957153257142
46	20.177527508431243	28.97189293968538	28.696479710202134	22.154099841681248
47	21.963477528681192	30.145072448514192	28.44525137704803	19.44619864575659
48	19.382581771294745	30.25296692308367	28.048277737331915	22.316173568289667
49	20.522412605967048	30.17204706625325	29.329134852797356	19.976405474982347
50	18.77433954320598	31.972950215766758	27.782163281058136	21.470546959969123
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	580.0
1	312.0
2	44.0
3	126.0
4	208.0
5	176.5
6	145.0
7	104.5
8	64.0
9	108.0
10	152.0
11	262.0
12	372.0
13	670.0
14	968.0
15	1222.5
16	1477.0
17	1690.5
18	1904.0
19	1897.0
20	1890.0
21	2105.5
22	2321.0
23	2316.5
24	2312.0
25	3221.5
26	4131.0
27	6253.5
28	8376.0
29	9763.0
30	11150.0
31	11645.5
32	12141.0
33	14441.0
34	16741.0
35	18998.0
36	21255.0
37	22744.5
38	24234.0
39	22067.0
40	19900.0
41	18639.0
42	17378.0
43	14435.0
44	11492.0
45	10980.5
46	10469.0
47	10245.0
48	10021.0
49	9029.5
50	8038.0
51	8036.5
52	8035.0
53	7481.0
54	6927.0
55	6413.0
56	5899.0
57	5786.0
58	5673.0
59	4990.0
60	4307.0
61	3817.0
62	3327.0
63	2671.5
64	2016.0
65	1803.0
66	1590.0
67	1261.5
68	933.0
69	786.0
70	639.0
71	521.0
72	403.0
73	314.0
74	225.0
75	184.5
76	144.0
77	122.5
78	101.0
79	62.5
80	24.0
81	18.0
82	12.0
83	6.0
84	0.0
85	1.0
86	2.0
87	2.0
88	2.0
89	2.5
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030698119082389366
2	0.005262534699838177
3	4.385445583198481E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	8.770891166396962E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0017541782332793924
25	0.0
26	4.385445583198481E-4
27	0.0
28	8.770891166396962E-4
29	0.0
30	0.0013156336749595443
31	0.0013156336749595443
32	4.385445583198481E-4
33	4.385445583198481E-4
34	0.0017541782332793924
35	0.0
36	0.0
37	0.0026312673499190886
38	0.0
39	4.385445583198481E-4
40	0.0
41	0.0021927227915992404
42	0.004823990141518329
43	0.0061396238164778735
44	0.004823990141518329
45	0.0021927227915992404
46	0.0026312673499190886
47	0.0013156336749595443
48	0.005701079258158026
49	0.003508356466558785
50	0.0013156336749595443
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	228027.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.03350041881006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.5153269199413	29.91487850123012
2	14.890754932333278	12.81602617233924
3	5.976887330833197	7.716191503637727
4	2.9400375020381544	5.060804203011047
5	1.6376569378770585	3.5237055260999792
6	1.0211152780042392	2.636529884618927
7	0.6715718245556824	2.023006047529459
8	0.509538561878363	1.7541782332793923
9	0.3648296103049079	1.4129905669065506
>10	2.1594244252405024	17.403202252364853
>50	0.19566280776129136	5.814662298762866
>100	0.11413663786075331	9.15242493213523
>500	0.0030572313712701775	0.7713998780846127
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	666	0.2920706758410188	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	585	0.2565485666171112	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	508	0.22278063562648281	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	471	0.20655448696864845	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	404	0.17717200156121862	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	357	0.15656040732018578	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	336	0.14735097159546898	No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT	325	0.14252698145395062	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC	315	0.13814153587075215	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	310	0.1359488130791529	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC	305	0.13375609028755367	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA	303	0.13287900117091397	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	303	0.13287900117091397	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	299	0.13112482293763458	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC	298	0.13068627837931474	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	296	0.12980918926267504	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	295	0.1293706447043552	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	288	0.12630083279611626	No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA	286	0.12542374367947656	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC	286	0.12542374367947656	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA	283	0.124108110004517	No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC	283	0.124108110004517	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT	281	0.12323102088787731	No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC	280	0.12279247632955746	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA	275	0.12059975353795824	No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC	265	0.11621430795475975	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	261	0.11446012972148034	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	251	0.11007468413828188	No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA	247	0.10832050590500247	No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG	245	0.10744341678836278	No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA	240	0.10525069399676354	No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG	238	0.10437360488012384	No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC	233	0.1021808820885246	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	233	0.1021808820885246	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA	230	0.10086524841356508	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	4.385445583198481E-4	0.0
21	0.0	0.0	0.0	4.385445583198481E-4	0.0
22	0.0	0.0	0.0	4.385445583198481E-4	0.0
23	0.0	0.0	0.0	4.385445583198481E-4	0.0
24	0.0	0.0	0.0	0.0013156336749595443	0.0
25	0.0	0.0	0.0	0.0021927227915992404	0.0
26	0.0	0.0	0.0	0.004385445583198481	0.0
27	0.0	0.0	0.0	0.005701079258158025	0.0
28	0.0	0.0	0.0	0.0074552574914374175	0.0
29	0.0	0.0	0.0	0.010086524841356507	0.0
30	0.0	0.0	0.0	0.016664693216154228	0.0
31	0.0	0.0	0.0	0.02806685173247028	0.0
32	0.0	0.0	0.0	0.040784643923745874	0.0
33	0.0	0.0	0.0	0.049555535090142835	0.0
34	0.0	0.0	0.0	0.06490459463133752	0.0
35	0.0	0.0	0.0	0.07718384226429327	0.0
36	0.0	0.0	0.0	0.10042670385524521	0.0
37	0.0	0.0	0.0	0.12366956544619716	0.0
38	0.0	0.0	0.0	0.15392913997026667	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATCGAA	25	0.002348919	35.19965	11
GTACTAC	35	3.2058012E-4	31.435152	1
TCACATA	30	0.0057356465	29.33304	43
CTAAACC	30	0.0057356465	29.33304	4
TGCGACC	30	0.0057356465	29.33304	12
TGTTCGG	30	0.0057356465	29.33304	10
ACTACAG	30	0.0057356465	29.33304	3
CTAGGTC	30	0.0057356465	29.33304	3
ATACATG	55	4.9298724E-6	28.005863	1
GTCCTAT	40	7.005943E-4	27.505758	1
GTATGGT	40	7.005943E-4	27.505758	1
GAATCGG	40	7.0149347E-4	27.499727	33
GATATAC	340	0.0	27.18216	1
GACCTAG	65	5.8962905E-7	27.07665	7
GGACCTA	75	6.960909E-8	26.399736	6
TAGGACC	380	0.0	26.052372	4
GTCCTAC	540	0.0	25.672043	1
TACACTA	60	9.708514E-6	25.66641	5
AGGACCT	420	0.0	25.142607	5
TCCTACA	560	0.0	24.755182	2
>>END_MODULE