FastQCFastQC Report
Thu 2 Feb 2017
SRR4062218_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062218_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1509690
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26200.1735455623339891No Hit
GCGCAAGACGGACCAGAGCGAAAGC20160.13353734872722214No Hit
GAATAGGACCGCGGTTCTATTTTGT16850.11161231776059986No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG16540.10955891606886181No Hit
TCGTAGTTCCGACCATAAACGATGC16190.10724055932012533No Hit
GATTAAGAGGGACGGCCGGGGGCAT16010.10604826156363227No Hit
GTCTTGCGCCGGTCCAAGAATTTCA15690.10392862110764461No Hit
ATCAGATACCGTCGTAGTTCCGACC15510.10273632335115156No Hit
GAACTACGACGGTATCTGATCGTCT15440.10227265200140426No Hit
GTATCTGATCGTCTTCGAACCTCCG15310.10141154806615926No Hit
GAATAACGCCGCCGCATCGCCAGTC15170.10048420536666468No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGTG400.00527692714.25084615
TATCGAG400.00527692714.25084610
AGTATCG400.00527808814.2503738
CGTGACG400.00528157414.24895518
GTATCGA500.001498840213.3007919
CCAATCG655.450938E-513.15419117
CGACCAT3300.012.95531510
CGAACGA905.4028715E-712.66699916
CCGACCA3550.012.5782119
CGGTCCA3650.012.23360310
CGGAATT1103.8098733E-812.09162715
AATCGGT550.003070057612.09002319
CTATTCC3050.011.8363764
CCGTCGT2250.011.8229259
GGTATCA8300.011.7855561
CGTCGTA2500.011.78069910
GTCCTAA1052.7302667E-711.7583121
GCGTTAT1800.011.6075651
GTATCAA18400.011.4584561
AAGACGG3650.011.4527355
Produced by FastQC (version 0.11.5)