##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062218_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1509690
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.177568242486867	32.0	32.0	32.0	32.0	32.0
2	30.708737555392165	32.0	32.0	32.0	32.0	32.0
3	30.728637004948034	32.0	32.0	32.0	32.0	32.0
4	30.72950340798442	32.0	32.0	32.0	32.0	32.0
5	30.58318661447052	32.0	32.0	32.0	32.0	32.0
6	34.25780325762243	36.0	36.0	36.0	32.0	36.0
7	34.1872046579099	36.0	36.0	36.0	32.0	36.0
8	34.118861488120075	36.0	36.0	36.0	32.0	36.0
9	34.278459816253665	36.0	36.0	36.0	32.0	36.0
10	33.88402850916413	36.0	36.0	36.0	32.0	36.0
11	34.29689538911962	36.0	36.0	36.0	32.0	36.0
12	34.03945644470057	36.0	36.0	36.0	32.0	36.0
13	34.16618047413707	36.0	36.0	36.0	32.0	36.0
14	34.03012406520544	36.0	36.0	36.0	32.0	36.0
15	33.96828156773907	36.0	36.0	36.0	32.0	36.0
16	33.97629910776384	36.0	36.0	36.0	32.0	36.0
17	33.886579363975386	36.0	36.0	36.0	32.0	36.0
18	33.89794328637005	36.0	36.0	36.0	32.0	36.0
19	33.901442680285356	36.0	36.0	36.0	32.0	36.0
20	33.89594751240321	36.0	36.0	36.0	32.0	36.0
21	33.87932158257656	36.0	36.0	36.0	32.0	36.0
22	33.83951539720075	36.0	36.0	36.0	32.0	36.0
23	33.800890248991514	36.0	36.0	36.0	32.0	36.0
24	33.77080592704463	36.0	36.0	36.0	32.0	36.0
25	33.40964701362531	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	13.0
5	110.0
6	332.0
7	91.0
8	266.0
9	193.0
10	115.0
11	34.0
12	51.0
13	60.0
14	248.0
15	339.0
16	488.0
17	700.0
18	935.0
19	1287.0
20	1948.0
21	2852.0
22	4488.0
23	6505.0
24	9437.0
25	13534.0
26	19112.0
27	25049.0
28	33839.0
29	45129.0
30	60056.0
31	81908.0
32	114808.0
33	159379.0
34	325756.0
35	600626.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.11359127542697	17.371844254395175	11.790480947122777	25.724083523055086
2	16.81029514824976	19.375338647476752	37.158951355318536	26.655414848954944
3	19.404165515761278	22.572252018803972	28.063743267617465	29.95983919781729
4	12.90003961476955	15.324158782355926	35.12796630492243	36.647835297952085
5	15.39573703405284	35.81610989779907	33.01154796234256	15.776605105805523
6	35.18346677978684	34.59846757516305	16.399729572087598	13.818336072962511
7	31.256216497224294	29.921874378350278	19.75267955896356	19.06922956546187
8	28.61524077291355	32.4721164940387	18.918013872127105	19.994628860920653
9	26.987480718396405	14.550034020507802	18.539585148755165	39.92290011234063
10	16.607249571036206	26.219288546882247	30.391529366194803	26.781932515886748
11	37.411813712354174	21.260224833576988	21.75359046540073	19.57437098866811
12	24.811139925583493	23.43702121732671	28.09919547929669	23.652643377793105
13	29.19371772194798	19.256703654404404	25.02770598429364	26.521872639353973
14	23.86392800229718	19.497565216583983	24.666746687693188	31.971760093425655
15	25.361337426014703	26.81186046326535	21.87079666678163	25.956005443938313
16	26.158029292684787	25.902970054082374	23.0617719521051	24.877228701127738
17	24.281958706822685	25.97708528423214	24.73226815363842	25.008687855306754
18	25.087431947640297	24.98086908085383	25.54405416337871	24.38764480812716
19	25.77009398465083	24.994413963433836	25.020006961825985	24.215485090089345
20	25.98850878018832	24.209067004711322	24.542625318077125	25.259798897023234
21	26.91591647737896	24.081573388601367	24.262573543744356	24.73993659027532
22	26.0938148554333	24.061009361063334	24.816498492867478	25.028677290635887
23	24.546818982159145	24.128375654977834	25.28095101720444	26.043854345658584
24	24.82420815769619	24.8496006810235	25.0645420925826	25.261649068697718
25	24.99119909146779	24.313446263409368	25.00319882362847	25.69215582149437
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	646.0
1	646.0
2	600.0
3	554.0
4	554.0
5	554.0
6	1049.0
7	1544.0
8	1544.0
9	1544.0
10	1848.0
11	2152.0
12	2152.0
13	2152.0
14	2967.0
15	3782.0
16	3782.0
17	3782.0
18	6424.0
19	9066.0
20	9066.0
21	9066.0
22	14287.5
23	19509.0
24	19509.0
25	19509.0
26	28652.5
27	37796.0
28	37796.0
29	37796.0
30	47382.0
31	56968.0
32	56968.0
33	56968.0
34	71933.0
35	86898.0
36	86898.0
37	86898.0
38	102635.0
39	118372.0
40	118372.0
41	118372.0
42	137643.0
43	156914.0
44	156914.0
45	156914.0
46	175306.5
47	193699.0
48	193699.0
49	193699.0
50	201423.5
51	209148.0
52	209148.0
53	209148.0
54	195701.5
55	182255.0
56	182255.0
57	182255.0
58	167875.0
59	153495.0
60	153495.0
61	153495.0
62	136066.5
63	118638.0
64	118638.0
65	118638.0
66	97675.0
67	76712.0
68	76712.0
69	76712.0
70	57865.5
71	39019.0
72	39019.0
73	39019.0
74	29749.5
75	20480.0
76	20480.0
77	20480.0
78	16660.5
79	12841.0
80	12841.0
81	12841.0
82	8937.0
83	5033.0
84	5033.0
85	5033.0
86	3772.0
87	2511.0
88	2511.0
89	2511.0
90	1664.0
91	817.0
92	817.0
93	817.0
94	505.5
95	194.0
96	194.0
97	194.0
98	420.5
99	647.0
100	647.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0015234915777411256
2	5.299101139969132E-4
3	5.961488782465274E-4
4	0.010068292165941352
5	0.033516814710304765
6	0.06425160132212573
7	0.10637945538488035
8	0.10796918572687107
9	0.11764004530731476
10	0.13161642456398334
11	0.13817406222469514
12	0.12982797792924375
13	0.1253899807245196
14	0.11611655372957361
15	0.1296955004007445
16	0.11956096947055356
17	0.12174684869079083
18	0.10995634865435952
19	0.09703978962568474
20	0.09326418006345673
21	0.09299922500645828
22	0.10121283177341044
23	0.08226854519802079
24	0.09087958455047063
25	0.08763388510223953
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1509690.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.993127094368695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.99182989103763	41.330309537381396
2	13.079818714185961	13.862809909843177
3	3.942448969268581	6.267680978745384
4	1.6038349249184254	3.399689120583576
5	0.8214851464337385	2.176653338554959
6	0.5184478968455755	1.648450517360744
7	0.34045418319069526	1.2629212259743798
8	0.254969919909238	1.0809322696793005
9	0.19043095964209877	0.9082378842314702
>10	1.082590870978627	10.527726267265539
>50	0.08464689302254272	3.098089210272438
>100	0.07587360614379987	8.353282677622232
>500	0.009656551243646528	3.4807666402393975
>1k	0.003511473179507828	2.602450422246006
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2620	0.1735455623339891	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2016	0.13353734872722214	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1685	0.11161231776059986	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1654	0.10955891606886181	No Hit
TCGTAGTTCCGACCATAAACGATGC	1619	0.10724055932012533	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1601	0.10604826156363227	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1569	0.10392862110764461	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1551	0.10273632335115156	No Hit
GAACTACGACGGTATCTGATCGTCT	1544	0.10227265200140426	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1531	0.10141154806615926	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1517	0.10048420536666468	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	6.623876424961415E-5	0.0
8	0.0	0.0	0.0	6.623876424961415E-5	0.0
9	0.0	0.0	0.0	6.623876424961415E-5	0.0
10	0.0	0.0	0.0	6.623876424961415E-5	0.0
11	0.0	0.0	0.0	6.623876424961415E-5	0.0
12	0.0	0.0	0.0	6.623876424961415E-5	0.0
13	0.0	0.0	0.0	6.623876424961415E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGTG	40	0.005276927	14.250846	15
TATCGAG	40	0.005276927	14.250846	10
AGTATCG	40	0.005278088	14.250373	8
CGTGACG	40	0.005281574	14.248955	18
GTATCGA	50	0.0014988402	13.300791	9
CCAATCG	65	5.450938E-5	13.154191	17
CGACCAT	330	0.0	12.955315	10
CGAACGA	90	5.4028715E-7	12.666999	16
CCGACCA	355	0.0	12.578211	9
CGGTCCA	365	0.0	12.233603	10
CGGAATT	110	3.8098733E-8	12.091627	15
AATCGGT	55	0.0030700576	12.090023	19
CTATTCC	305	0.0	11.836376	4
CCGTCGT	225	0.0	11.822925	9
GGTATCA	830	0.0	11.785556	1
CGTCGTA	250	0.0	11.780699	10
GTCCTAA	105	2.7302667E-7	11.758312	1
GCGTTAT	180	0.0	11.607565	1
GTATCAA	1840	0.0	11.458456	1
AAGACGG	365	0.0	11.452735	5
>>END_MODULE