FastQCFastQC Report
Thu 2 Feb 2017
SRR4062218_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062218_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1509690
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT20170.13360358749147178No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA18020.11936225317780473No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17890.11850114924255974No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA17570.11638150878657207No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT16740.1108836913538541No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG16570.10975763236161067No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC16260.10770423066987261No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA15920.10545211268538573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19050.025.7629971
TACCGTG703.2173644E-521.9988237
GGTATCA9900.020.6744631
ATACCGT4950.020.443356
GTATTAC1850.020.2238431
TACCGTC5150.019.2222757
ACCGTCG5100.018.9793788
ATCGATT1051.79364E-618.8567629
CGTCGTA5500.018.79899410
CGCAATA3950.018.3818136
CCGTCGT5350.018.0924919
CGGATCG1102.798788E-617.99903926
GTAATAC1355.5315468E-817.9249693
ACGCTTA1002.4848177E-517.5990617
CTCGACG1002.4848177E-517.5990613
TACGAAT4300.017.39787741
GTCGTAG5700.017.36749311
CTAGCGG4200.017.28536229
TCAACGC29400.016.9854874
ATCAACG29550.016.9737133
Produced by FastQC (version 0.11.5)