##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062218_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1509690
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.912547609111805	32.0	32.0	32.0	32.0	32.0
2	31.302297160344178	32.0	32.0	32.0	32.0	32.0
3	31.48038935145626	32.0	32.0	32.0	32.0	32.0
4	31.588789751538396	32.0	32.0	32.0	32.0	32.0
5	31.52082016837894	32.0	32.0	32.0	32.0	32.0
6	35.186398532148985	36.0	36.0	36.0	36.0	36.0
7	35.19069345362293	36.0	36.0	36.0	36.0	36.0
8	35.12883969556664	36.0	36.0	36.0	36.0	36.0
9	35.24986189217654	36.0	36.0	36.0	36.0	36.0
10	35.08402122290006	36.0	36.0	36.0	36.0	36.0
11	35.26533857944346	36.0	36.0	36.0	36.0	36.0
12	35.1594208082454	36.0	36.0	36.0	36.0	36.0
13	35.214774556365875	36.0	36.0	36.0	36.0	36.0
14	35.16824977313223	36.0	36.0	36.0	36.0	36.0
15	35.133891726115955	36.0	36.0	36.0	36.0	36.0
16	35.14706396677464	36.0	36.0	36.0	36.0	36.0
17	35.12599474064212	36.0	36.0	36.0	36.0	36.0
18	35.12844358775643	36.0	36.0	36.0	36.0	36.0
19	35.128711854751636	36.0	36.0	36.0	36.0	36.0
20	35.12899933098848	36.0	36.0	36.0	36.0	36.0
21	35.11113738582093	36.0	36.0	36.0	36.0	36.0
22	35.099933098848105	36.0	36.0	36.0	36.0	36.0
23	35.06665143175089	36.0	36.0	36.0	36.0	36.0
24	35.04341023653863	36.0	36.0	36.0	36.0	36.0
25	35.03414210864482	36.0	36.0	36.0	36.0	36.0
26	34.98185720247203	36.0	36.0	36.0	36.0	36.0
27	34.97656207565792	36.0	36.0	36.0	36.0	36.0
28	34.958721989282566	36.0	36.0	36.0	36.0	36.0
29	34.92531976763441	36.0	36.0	36.0	32.0	36.0
30	34.90643310878392	36.0	36.0	36.0	32.0	36.0
31	34.9139565076274	36.0	36.0	36.0	32.0	36.0
32	34.886921818386554	36.0	36.0	36.0	32.0	36.0
33	34.86341434334201	36.0	36.0	36.0	32.0	36.0
34	34.853124813703474	36.0	36.0	36.0	32.0	36.0
35	34.83952135868953	36.0	36.0	36.0	32.0	36.0
36	34.810521365313406	36.0	36.0	36.0	32.0	36.0
37	34.79914088322768	36.0	36.0	36.0	32.0	36.0
38	34.78174989567395	36.0	36.0	36.0	32.0	36.0
39	34.77363697182865	36.0	36.0	36.0	32.0	36.0
40	34.758792202372675	36.0	36.0	36.0	32.0	36.0
41	34.72986109731137	36.0	36.0	36.0	32.0	36.0
42	34.6853777927919	36.0	36.0	36.0	32.0	36.0
43	34.69046492988627	36.0	36.0	36.0	32.0	36.0
44	34.634010955891604	36.0	36.0	36.0	32.0	36.0
45	34.62223569077095	36.0	36.0	36.0	32.0	36.0
46	34.59743987176175	36.0	36.0	36.0	32.0	36.0
47	34.57492664056859	36.0	36.0	36.0	32.0	36.0
48	34.55028582026774	36.0	36.0	36.0	32.0	36.0
49	34.52908146705615	36.0	36.0	36.0	32.0	36.0
50	34.102512436327984	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	4.0
20	21.0
21	37.0
22	91.0
23	265.0
24	699.0
25	1726.0
26	3668.0
27	6955.0
28	11919.0
29	19261.0
30	29408.0
31	44353.0
32	69967.0
33	122615.0
34	278881.0
35	919819.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.457680753425834	18.216006986177387	12.28246048079	26.043851779606776
2	16.30862253641107	19.848413890226592	36.651923608125664	27.191039965236673
3	18.719045735970436	22.958533788791982	28.438278928016203	29.884141547221375
4	12.725990103928622	15.81476991965238	35.26737277189357	36.19186720452543
5	15.060850281084381	36.17016484852178	32.79119076842979	15.977794101964045
6	35.3509272789176	34.82247301756789	16.148523600271844	13.678076103242661
7	31.116056938841748	30.04696328385298	19.719876265988383	19.117103511316895
8	28.51148248978267	32.814617570494605	18.686021633580406	19.98787830614232
9	27.140671263636907	14.300750485198948	18.404506885519545	40.1540713656446
10	16.586120329339135	26.28837708403712	30.459432068835323	26.66607051778842
11	37.6913803496082	21.111618941637026	21.62940736177626	19.56759334697852
12	24.892858798826246	23.334326914797078	28.10504143234704	23.667772854029636
13	29.333505554120382	19.1503553709702	25.10575018712451	26.410388887784908
14	23.947697871748506	19.283164093290676	24.642873702548204	32.12626433241261
15	25.281614106207233	26.905391173022274	21.905887963754147	25.90710675701634
16	26.23373664122643	25.811727237921627	23.072474865567717	24.882061255284224
17	24.357583344925118	25.96612549596275	24.628036219356293	25.048254939755843
18	25.214895537949257	24.90257914388877	25.573115486852572	24.309409831309402
19	25.80066106286721	24.858547118944948	25.156886513125208	24.183905305062627
20	26.073616584099884	23.98735631955498	24.6844544597655	25.254572636579635
21	26.97189489232889	23.994131245487484	24.34837615669442	24.685597705489208
22	26.083434347448815	23.997310706171465	24.97360385244653	24.94565109393319
23	24.558949188243943	24.01022726520014	25.389517053169858	26.041306493386056
24	24.884975471590632	24.628960612792927	25.26002233588884	25.2260415797276
25	25.19096661031948	24.063912543518924	25.271314337796944	25.473806508364643
26	24.86339865601102	25.165382182375794	25.422853111741855	24.54836604987133
27	25.44168183540397	24.509963674517316	24.931972518752268	25.11638197132645
28	24.99576063484493	24.379462925426918	25.509916140058024	25.114860299670124
29	24.73997904199946	24.732096608168852	25.4523126001036	25.07561174972809
30	24.823737013769843	24.786046807394435	25.59959885432371	24.790617324512016
31	25.4102340418908	24.56873510866822	24.612452779947102	25.408578069493874
32	25.319652775247814	24.508291464775763	24.558500614366572	25.613555145609844
33	24.80931477849382	24.23449364007241	25.170647955503362	25.78554362593041
34	25.532630842644384	24.414311415164576	25.287412663379428	24.765645078811612
35	26.123145974532484	24.074974431803128	25.326491301513954	24.47538829215043
36	24.831521256738156	24.845100239387527	24.970556791279776	25.35282171259454
37	25.873740940000978	24.617694868639123	24.85337738746127	24.655186803898633
38	25.063440218482086	24.28705514844448	25.217511686181727	25.43199294689171
39	25.693193259498702	24.27666790313179	24.719543214456046	25.310595622913468
40	25.76530662937863	24.582943904340564	24.98918651457353	24.662562951707272
41	24.767300446719553	25.02417743721815	25.365576098649246	24.842946017413052
42	25.99776761316777	24.89243803801682	24.986503002460925	24.123291346354485
43	25.54084766971313	23.945215388498173	25.171748405725676	25.342188536063027
44	24.992349159202405	24.72182072788311	24.82595165198544	25.45987846092904
45	25.203424365350426	24.89295546258343	24.88487412993193	25.018746042134214
46	25.015864604378496	24.7103633292485	24.892855959990726	25.380916106382273
47	25.141934334298	24.52504741080377	25.25692581923153	25.076092435666702
48	26.043303383350068	25.089492245771805	24.220403967070034	24.646800403808093
49	25.090403964459767	25.223484429453112	24.457857742348533	25.228253863738587
50	25.441222838398907	25.14884067225377	24.37588926836894	25.03404722097839
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	199.0
1	128.5
2	58.0
3	156.5
4	255.0
5	259.5
6	264.0
7	220.0
8	176.0
9	245.0
10	314.0
11	498.5
12	683.0
13	1292.0
14	1901.0
15	2552.5
16	3204.0
17	3075.5
18	2947.0
19	2806.0
20	2665.0
21	3157.0
22	3649.0
23	4446.0
24	5243.0
25	6540.0
26	7837.0
27	10040.5
28	12244.0
29	14329.5
30	16415.0
31	18756.0
32	21097.0
33	24267.5
34	27438.0
35	31872.5
36	36307.0
37	43871.0
38	51435.0
39	57394.5
40	63354.0
41	69436.0
42	75518.0
43	78980.5
44	82443.0
45	90495.0
46	98547.0
47	105413.0
48	112279.0
49	116657.5
50	121036.0
51	117469.0
52	113902.0
53	113901.0
54	113900.0
55	114524.0
56	115148.0
57	111349.5
58	107551.0
59	98336.5
60	89122.0
61	77740.5
62	66359.0
63	57404.0
64	48449.0
65	40614.5
66	32780.0
67	28434.0
68	24088.0
69	21739.0
70	19390.0
71	15146.0
72	10902.0
73	9614.0
74	8326.0
75	6098.0
76	3870.0
77	3486.0
78	3102.0
79	2652.0
80	2202.0
81	1746.5
82	1291.0
83	1117.0
84	943.0
85	721.0
86	499.0
87	352.0
88	205.0
89	136.0
90	67.0
91	46.0
92	25.0
93	21.0
94	17.0
95	21.5
96	26.0
97	18.0
98	10.0
99	9.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.028085236041836405
2	0.0035106545052295507
3	1.9871629274884246E-4
4	0.0
5	6.623876424961415E-5
6	2.649550569984566E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.299101139969132E-4
17	0.0
18	1.9871629274884246E-4
19	0.0
20	1.9871629274884246E-4
21	0.0
22	0.0
23	0.0
24	7.948651709953698E-4
25	1.324775284992283E-4
26	3.3119382124807077E-4
27	3.974325854976849E-4
28	0.001987162927488425
29	2.649550569984566E-4
30	9.273426994945981E-4
31	1.9871629274884246E-4
32	3.3119382124807077E-4
33	1.9871629274884246E-4
34	9.273426994945981E-4
35	1.324775284992283E-4
36	2.649550569984566E-4
37	0.002119640455987653
38	6.623876424961415E-5
39	6.623876424961415E-4
40	6.623876424961415E-5
41	0.0011922977564930548
42	0.006292682603713346
43	0.004570474733223377
44	0.0034444157409799364
45	0.002517073041485338
46	0.002649550569984566
47	8.611039352449841E-4
48	0.004106803383476078
49	0.00510038484722029
50	0.0014572528134915114
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1509690.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.238909329770294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.53819713031338	42.05587243744923
2	13.537644413350705	14.685341357488008
3	3.9939949795866876	6.498897946840803
4	1.6285979217366804	3.533335000469126
5	0.8359475280795003	2.2670441089974815
6	0.5062563763558008	1.6475276216868204
7	0.3435520183652593	1.3043720741921014
8	0.23596817708299656	1.0238925249212665
9	0.19338970487811	0.9440322001375361
>10	1.0272105186851639	10.088819905118086
>50	0.08048960596845951	3.027023302598403
>100	0.066362469167071	7.306601826926851
>500	0.009445198466613429	3.4145610576116496
>1k	0.00294395796361977	2.2026786355627
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2017	0.13360358749147178	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1802	0.11936225317780473	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1789	0.11850114924255974	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1757	0.11638150878657207	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1674	0.1108836913538541	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1657	0.10975763236161067	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1626	0.10770423066987261	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1592	0.10545211268538573	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	6.623876424961415E-5
6	0.0	0.0	0.0	0.0	6.623876424961415E-5
7	0.0	0.0	0.0	0.0	6.623876424961415E-5
8	0.0	0.0	0.0	0.0	6.623876424961415E-5
9	0.0	0.0	0.0	0.0	6.623876424961415E-5
10	0.0	0.0	0.0	0.0	6.623876424961415E-5
11	0.0	0.0	0.0	0.0	6.623876424961415E-5
12	0.0	0.0	0.0	0.0	6.623876424961415E-5
13	0.0	0.0	0.0	0.0	1.987162927488425E-4
14	0.0	0.0	0.0	6.623876424961415E-5	1.987162927488425E-4
15	0.0	0.0	0.0	6.623876424961415E-5	3.311938212480708E-4
16	0.0	0.0	0.0	1.324775284992283E-4	3.311938212480708E-4
17	0.0	0.0	0.0	2.649550569984566E-4	3.311938212480708E-4
18	0.0	0.0	0.0	4.6367134974729913E-4	3.311938212480708E-4
19	0.0	1.324775284992283E-4	0.0	5.961488782465274E-4	3.311938212480708E-4
20	0.0	1.324775284992283E-4	0.0	6.623876424961416E-4	3.311938212480708E-4
21	0.0	1.324775284992283E-4	0.0	6.623876424961416E-4	3.97432585497685E-4
22	0.0	1.324775284992283E-4	0.0	7.286264067457558E-4	4.6367134974729913E-4
23	6.623876424961415E-5	1.324775284992283E-4	0.0	0.0013247752849922833	4.6367134974729913E-4
24	6.623876424961415E-5	1.324775284992283E-4	0.0	0.00158973034199074	4.6367134974729913E-4
25	6.623876424961415E-5	1.324775284992283E-4	0.0	0.001987162927488425	4.6367134974729913E-4
26	6.623876424961415E-5	1.324775284992283E-4	0.0	0.0026495505699845666	4.6367134974729913E-4
27	6.623876424961415E-5	1.324775284992283E-4	0.0	0.0031132219197318655	4.6367134974729913E-4
28	6.623876424961415E-5	1.324775284992283E-4	0.0	0.003908087090727236	4.6367134974729913E-4
29	6.623876424961415E-5	1.324775284992283E-4	0.0	0.005762772489716432	4.6367134974729913E-4
30	6.623876424961415E-5	1.324775284992283E-4	0.0	0.008544800588200226	4.6367134974729913E-4
31	6.623876424961415E-5	1.324775284992283E-4	0.0	0.014241334313667045	4.6367134974729913E-4
32	6.623876424961415E-5	1.324775284992283E-4	0.0	0.02166007590962383	4.6367134974729913E-4
33	6.623876424961415E-5	1.324775284992283E-4	0.0	0.02755532592783949	4.6367134974729913E-4
34	6.623876424961415E-5	1.324775284992283E-4	0.0	0.034311679881300135	5.299101139969132E-4
35	6.623876424961415E-5	1.324775284992283E-4	0.0	0.04219409282700422	5.299101139969132E-4
36	6.623876424961415E-5	1.324775284992283E-4	0.0	0.053653399042187466	5.299101139969132E-4
37	6.623876424961415E-5	1.324775284992283E-4	0.0	0.07114043280408561	5.299101139969132E-4
38	6.623876424961415E-5	1.324775284992283E-4	0.0	0.09492014916969709	5.299101139969132E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1905	0.0	25.762997	1
TACCGTG	70	3.2173644E-5	21.998823	7
GGTATCA	990	0.0	20.674463	1
ATACCGT	495	0.0	20.44335	6
GTATTAC	185	0.0	20.223843	1
TACCGTC	515	0.0	19.222275	7
ACCGTCG	510	0.0	18.979378	8
ATCGATT	105	1.79364E-6	18.85676	29
CGTCGTA	550	0.0	18.798994	10
CGCAATA	395	0.0	18.38181	36
CCGTCGT	535	0.0	18.092491	9
CGGATCG	110	2.798788E-6	17.999039	26
GTAATAC	135	5.5315468E-8	17.924969	3
ACGCTTA	100	2.4848177E-5	17.59906	17
CTCGACG	100	2.4848177E-5	17.59906	13
TACGAAT	430	0.0	17.397877	41
GTCGTAG	570	0.0	17.367493	11
CTAGCGG	420	0.0	17.285362	29
TCAACGC	2940	0.0	16.985487	4
ATCAACG	2955	0.0	16.973713	3
>>END_MODULE