FastQCFastQC Report
Thu 2 Feb 2017
SRR4062217_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062217_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36869
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT1380.37429819089207733No Hit
TATCAACGCAGAGTACTTTTTTTTT830.22512137568146678No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT800.21698445848816078No Hit
ACCATACTCCCCCCGGAACCCAAAG720.1952860126393447No Hit
GCGCAAGACGGACCAGAGCGAAAGC660.17901217825273266No Hit
GGCTCCACTCCTGGTGGTGCCCTTC640.1735875667905286No Hit
CTCCCGACCCGGGGAGGTAGTGACG640.1735875667905286No Hit
CTCCACTCCTGGTGGTGCCCTTCCG620.1681629553283246No Hit
GTCAATCCTGTCCGTGTCCGGGCCG600.1627383438661206No Hit
ATACAGGACTCTTTCGAGGCCCTGT560.15188912094171253No Hit
TTCGTATTGCGCCGCTAGAGGTGAA540.14646450947950854No Hit
GGTATCAACGCAGAGTACTTTTTTT480.13019067509289647No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG450.12205375789959044No Hit
CTATTGGAGCTGGAATTACCGCGGC440.11934145216848843No Hit
CTTCGGGCCCCGCGGGACACTCAGC400.10849222924408039No Hit
ACGCAGAGTACTTTTTTTTTTTTTT390.10577992351297838No Hit
CTCTAGATAACCTCGGGCCGATCGC380.10306761778187637No Hit
GAATAGGACCGCGGTTCTATTTTGT370.10035531205077437No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA453.390158E-516.888891
AGTACAT855.0037866E-511.17647112
GAGTACA855.0037866E-511.17647111
ACATGGG951.2632458E-511.015
GTACATG908.949975E-510.55555513
TACATGG908.949975E-510.55555514
TATCAAC1053.8173406E-59.9523811
TCAACGC1106.361394E-59.53
ATCAACG1106.361394E-59.52
AACGCAG1151.0341176E-49.0869565
CAACGCA1151.0341176E-49.0869564
ACGCAGA1252.5576688E-48.366
AGAGTAC1252.5576688E-48.3610
CAGAGTA1303.9049602E-48.0384629
GCAGAGT1303.9049602E-48.0384628
CGCAGAG1408.6416E-47.4642867
Produced by FastQC (version 0.11.5)