##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062217_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	36869
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.49429059643603	32.0	14.0	32.0	14.0	32.0
2	24.131926550760802	32.0	14.0	32.0	14.0	32.0
3	24.563861238438797	32.0	14.0	32.0	14.0	32.0
4	24.06807887385066	27.0	14.0	32.0	14.0	32.0
5	24.568906127098646	32.0	14.0	32.0	14.0	32.0
6	28.222490439122296	32.0	21.0	36.0	14.0	36.0
7	27.654425126800295	32.0	21.0	36.0	14.0	36.0
8	27.302476335132496	32.0	21.0	36.0	14.0	36.0
9	27.376929127451245	32.0	14.0	36.0	14.0	36.0
10	26.002956413246903	32.0	14.0	36.0	14.0	36.0
11	28.53049987794624	32.0	21.0	36.0	14.0	36.0
12	26.96091567441482	32.0	14.0	36.0	14.0	36.0
13	27.56188125525509	32.0	21.0	36.0	14.0	36.0
14	26.809569014619328	32.0	14.0	36.0	14.0	36.0
15	26.916569475711302	32.0	14.0	36.0	14.0	36.0
16	27.025631289158913	32.0	14.0	36.0	14.0	36.0
17	26.728905042176354	32.0	14.0	36.0	14.0	36.0
18	26.772763025848274	32.0	14.0	36.0	14.0	36.0
19	26.737231820770837	32.0	14.0	36.0	14.0	36.0
20	26.79871979169492	32.0	14.0	36.0	14.0	36.0
21	26.808863815129243	32.0	14.0	36.0	14.0	36.0
22	26.53361902953701	32.0	14.0	36.0	14.0	36.0
23	26.28715180775177	32.0	14.0	36.0	14.0	36.0
24	26.325530933846863	32.0	14.0	36.0	14.0	36.0
25	25.52540074317177	27.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	3.0
5	4.0
6	8.0
7	3.0
8	2.0
9	1.0
10	2.0
11	2.0
12	2.0
13	1.0
14	83.0
15	211.0
16	486.0
17	913.0
18	1550.0
19	2063.0
20	2495.0
21	2462.0
22	2258.0
23	2038.0
24	1758.0
25	1747.0
26	1691.0
27	1604.0
28	1538.0
29	1575.0
30	1638.0
31	1708.0
32	1898.0
33	2030.0
34	2720.0
35	2375.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.88445577585505	19.31161680544631	12.655618541321978	28.14830887737666
2	16.29010822099867	18.698635710217257	38.598822859312705	26.41243320947137
3	18.459952805880278	21.882882638531015	27.147468062600016	32.50969649298869
4	12.061950743191927	14.48410545730715	34.18411630682435	39.269827492676576
5	15.078676071622354	35.2197504069452	32.84319045035269	16.85838307107976
6	35.67241940735917	34.59513730598068	15.440138934114838	14.292304352545315
7	30.921231203517724	29.230226372075347	19.703056294446554	20.14548612996037
8	27.404511768059287	32.478214838341884	18.785460270923256	21.331813122675573
9	25.912151156477357	13.777283092626776	18.232164187208166	42.07840156368769
10	15.485692566650378	25.15339088885269	30.952380952380953	28.40853559211598
11	37.54989003828297	20.1786538513752	21.5687871629877	20.70266894735413
12	24.58522279849024	22.49979634507291	28.31618106280718	24.598799793629674
13	28.80145541435864	18.312153796024763	25.029868578255677	27.856522211360925
14	23.35695089176643	18.190949317262533	25.42552325107908	33.026576539891956
15	25.3713106519319	26.147872600396425	21.865920877569305	26.614895870102366
16	25.82396698702286	25.6882228375957	23.193245371124505	25.294564804256936
17	23.304555573654774	26.388662648639844	25.54976380518	24.757017972525382
18	24.433946896888745	24.322636694358472	26.157897594613672	25.085518814139114
19	25.090928831225234	24.82492807122306	24.8357852451007	25.248357852451008
20	25.238844859407227	23.887200086852676	24.723157094777985	26.150797958962112
21	25.961512363271176	23.40200309421057	25.147246424015417	25.48923811850284
22	24.631544662486768	23.809136063838448	25.81494449420514	25.74437477946964
23	23.068154059115706	24.11584290095812	26.420215509051925	26.395787530874248
24	23.850621505726536	24.293003311078543	25.72870867936818	26.12766650382674
25	24.489685124864277	23.797502714440824	25.719326818675352	25.993485342019547
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	3.0
2	9.0
3	15.0
4	15.0
5	15.0
6	31.5
7	48.0
8	48.0
9	48.0
10	49.0
11	50.0
12	50.0
13	50.0
14	79.5
15	109.0
16	109.0
17	109.0
18	201.0
19	293.0
20	293.0
21	293.0
22	428.0
23	563.0
24	563.0
25	563.0
26	877.0
27	1191.0
28	1191.0
29	1191.0
30	1419.0
31	1647.0
32	1647.0
33	1647.0
34	2000.0
35	2353.0
36	2353.0
37	2353.0
38	2554.0
39	2755.0
40	2755.0
41	2755.0
42	3096.0
43	3437.0
44	3437.0
45	3437.0
46	3845.5
47	4254.0
48	4254.0
49	4254.0
50	4444.5
51	4635.0
52	4635.0
53	4635.0
54	4345.5
55	4056.0
56	4056.0
57	4056.0
58	3864.5
59	3673.0
60	3673.0
61	3673.0
62	3293.5
63	2914.0
64	2914.0
65	2914.0
66	2516.0
67	2118.0
68	2118.0
69	2118.0
70	1667.0
71	1216.0
72	1216.0
73	1216.0
74	953.5
75	691.0
76	691.0
77	691.0
78	580.5
79	470.0
80	470.0
81	470.0
82	342.0
83	214.0
84	214.0
85	214.0
86	156.5
87	99.0
88	99.0
89	99.0
90	60.0
91	21.0
92	21.0
93	21.0
94	14.0
95	7.0
96	7.0
97	7.0
98	22.0
99	37.0
100	37.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0027123057311020096
5	0.024410751579918087
6	0.04610919742873417
7	0.07323225473975427
8	0.0867937833952643
9	0.08950608912636633
10	0.09493070058857034
11	0.10306761778187637
12	0.1139168407062844
13	0.1112045349751824
14	0.0867937833952643
15	0.10849222924408039
16	0.09493070058857034
17	0.09493070058857034
18	0.09493070058857034
19	0.07323225473975427
20	0.06780764327755025
21	0.07051994900865226
22	0.07051994900865226
23	0.07051994900865226
24	0.06238303181534623
25	0.07865686620195829
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	36869.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.19932734817868
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.97602739726027	72.84439502020668
2	4.88013698630137	7.730071333640728
3	1.0821917808219177	2.5712658330847056
4	0.5273972602739726	1.670780330358838
5	0.3219178082191781	1.2747836936179446
6	0.2226027397260274	1.0577992351297838
7	0.1506849315068493	0.835390165179419
8	0.1232876712328767	0.7811440505573788
9	0.10273972602739725	0.7323225473975427
>10	0.5753424657534246	8.334915511676476
>50	0.03424657534246575	1.7928340882584284
>100	0.003424657534246575	0.37429819089207733
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	138	0.37429819089207733	No Hit
TATCAACGCAGAGTACTTTTTTTTT	83	0.22512137568146678	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	80	0.21698445848816078	No Hit
ACCATACTCCCCCCGGAACCCAAAG	72	0.1952860126393447	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	66	0.17901217825273266	No Hit
GGCTCCACTCCTGGTGGTGCCCTTC	64	0.1735875667905286	No Hit
CTCCCGACCCGGGGAGGTAGTGACG	64	0.1735875667905286	No Hit
CTCCACTCCTGGTGGTGCCCTTCCG	62	0.1681629553283246	No Hit
GTCAATCCTGTCCGTGTCCGGGCCG	60	0.1627383438661206	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	56	0.15188912094171253	No Hit
TTCGTATTGCGCCGCTAGAGGTGAA	54	0.14646450947950854	No Hit
GGTATCAACGCAGAGTACTTTTTTT	48	0.13019067509289647	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	45	0.12205375789959044	No Hit
CTATTGGAGCTGGAATTACCGCGGC	44	0.11934145216848843	No Hit
CTTCGGGCCCCGCGGGACACTCAGC	40	0.10849222924408039	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	39	0.10577992351297838	No Hit
CTCTAGATAACCTCGGGCCGATCGC	38	0.10306761778187637	No Hit
GAATAGGACCGCGGTTCTATTTTGT	37	0.10035531205077437	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.00271230573110201	0.0
8	0.0	0.0	0.0	0.00271230573110201	0.0
9	0.0	0.0	0.0	0.00271230573110201	0.0
10	0.0	0.0	0.0	0.00271230573110201	0.0
11	0.0	0.0	0.0	0.00271230573110201	0.0
12	0.0	0.0	0.0	0.00271230573110201	0.0
13	0.0	0.0	0.0	0.00271230573110201	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	45	3.390158E-5	16.88889	1
AGTACAT	85	5.0037866E-5	11.176471	12
GAGTACA	85	5.0037866E-5	11.176471	11
ACATGGG	95	1.2632458E-5	11.0	15
GTACATG	90	8.949975E-5	10.555555	13
TACATGG	90	8.949975E-5	10.555555	14
TATCAAC	105	3.8173406E-5	9.952381	1
TCAACGC	110	6.361394E-5	9.5	3
ATCAACG	110	6.361394E-5	9.5	2
AACGCAG	115	1.0341176E-4	9.086956	5
CAACGCA	115	1.0341176E-4	9.086956	4
ACGCAGA	125	2.5576688E-4	8.36	6
AGAGTAC	125	2.5576688E-4	8.36	10
CAGAGTA	130	3.9049602E-4	8.038462	9
GCAGAGT	130	3.9049602E-4	8.038462	8
CGCAGAG	140	8.6416E-4	7.464286	7
>>END_MODULE