FastQCFastQC Report
Thu 2 Feb 2017
SRR4062217_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062217_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36869
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC1180.3200520762700372No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT810.2196967642192628No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG650.17629987252163062No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT620.1681629553283246No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG610.1654506495972226No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC580.15731373240391658No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG500.1356152865551005No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT490.13290298082399848No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC490.13290298082399848No Hit
GGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTT410.1112045349751824No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA410.1112045349751824No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC390.10577992351297838No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGC207.7575806E-444.030
TCTAGCG207.7575806E-444.028
TTTCACC207.7575806E-444.021
AGAATTT207.7575806E-444.017
GCAATAC207.7575806E-444.037
CCTCTAG207.7575806E-444.026
CACCTCT207.7575806E-444.024
AGCGGCG207.7575806E-444.031
ACCTCTA207.7575806E-444.025
CTAGCGG207.7575806E-444.029
CTTGCGC207.7575806E-444.03
GCGCAAT207.7575806E-444.035
CGCAATA207.7575806E-444.036
CGCCGGT254.3592616E-544.07
AATTTCA250.002323409835.219
TCGGAAC250.002323409835.240
CGGAACT250.002323409835.241
TCTTGCG250.002323409835.22
AATACGA250.002323409835.239
CGGTCCA250.002323409835.210
Produced by FastQC (version 0.11.5)