##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062217_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	36869
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.051235455260517	32.0	32.0	32.0	21.0	32.0
2	28.61203178822317	32.0	32.0	32.0	14.0	32.0
3	30.28346307195747	32.0	32.0	32.0	21.0	32.0
4	30.84895169383493	32.0	32.0	32.0	32.0	32.0
5	30.552550923540103	32.0	32.0	32.0	32.0	32.0
6	34.111231658032494	36.0	36.0	36.0	32.0	36.0
7	34.124332094713715	36.0	36.0	36.0	32.0	36.0
8	33.82616832569367	36.0	36.0	36.0	32.0	36.0
9	34.24719954433264	36.0	36.0	36.0	32.0	36.0
10	33.73175296319401	36.0	36.0	36.0	32.0	36.0
11	34.49982370012748	36.0	36.0	36.0	32.0	36.0
12	34.11885323713689	36.0	36.0	36.0	32.0	36.0
13	34.33930944696086	36.0	36.0	36.0	32.0	36.0
14	34.10404404784507	36.0	36.0	36.0	32.0	36.0
15	34.058097588760205	36.0	36.0	36.0	32.0	36.0
16	34.10138598822859	36.0	36.0	36.0	32.0	36.0
17	33.998643847134446	36.0	36.0	36.0	32.0	36.0
18	34.120426374460926	36.0	36.0	36.0	32.0	36.0
19	34.02465485909572	36.0	36.0	36.0	32.0	36.0
20	34.08082671078684	36.0	36.0	36.0	32.0	36.0
21	34.087119260083	36.0	36.0	36.0	32.0	36.0
22	34.04865876481597	36.0	36.0	36.0	32.0	36.0
23	33.98106810599691	36.0	36.0	36.0	32.0	36.0
24	33.86001790121782	36.0	36.0	36.0	32.0	36.0
25	33.917166182972146	36.0	36.0	36.0	32.0	36.0
26	33.836095364669504	36.0	36.0	36.0	32.0	36.0
27	33.79657707016735	36.0	36.0	36.0	32.0	36.0
28	33.66570831864168	36.0	36.0	36.0	27.0	36.0
29	33.667552686538826	36.0	36.0	36.0	27.0	36.0
30	33.61799886083159	36.0	36.0	36.0	27.0	36.0
31	33.67430632780927	36.0	36.0	36.0	32.0	36.0
32	33.584339146708615	36.0	36.0	36.0	27.0	36.0
33	33.526485665464214	36.0	36.0	36.0	21.0	36.0
34	33.4272695218205	36.0	36.0	36.0	21.0	36.0
35	33.458786514415905	36.0	36.0	36.0	21.0	36.0
36	33.38303181534623	36.0	36.0	36.0	21.0	36.0
37	33.34712088746644	36.0	36.0	36.0	21.0	36.0
38	33.34527651956929	36.0	36.0	36.0	21.0	36.0
39	33.35729203395807	36.0	36.0	36.0	21.0	36.0
40	33.29501749437197	36.0	36.0	36.0	21.0	36.0
41	33.193441644742194	36.0	36.0	36.0	21.0	36.0
42	33.126474816241284	36.0	36.0	36.0	21.0	36.0
43	33.22048333288128	36.0	36.0	36.0	21.0	36.0
44	33.0718761018742	36.0	36.0	36.0	21.0	36.0
45	33.04157964685779	36.0	36.0	36.0	14.0	36.0
46	32.94692017684233	36.0	36.0	36.0	14.0	36.0
47	33.06482410697334	36.0	36.0	36.0	14.0	36.0
48	32.97287694268898	36.0	36.0	36.0	14.0	36.0
49	32.872141907835854	36.0	36.0	36.0	14.0	36.0
50	32.15031598361767	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	3.0
22	15.0
23	20.0
24	59.0
25	107.0
26	232.0
27	410.0
28	799.0
29	1278.0
30	2024.0
31	3240.0
32	4621.0
33	7085.0
34	9558.0
35	7417.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.26479683176911	18.49671784299897	13.418868333966255	24.819616991265665
2	19.67831181512423	19.339264402734077	34.99511771726158	25.987306064880112
3	22.75624508394586	20.686755811115027	27.470232444601155	29.086766660337954
4	16.523366513873444	15.698825571618432	34.88567631343405	32.89213160107407
5	21.40551682985706	30.936559168949522	30.223222761669692	17.43470123952372
6	37.267080745341616	34.72022566383683	13.789362336922617	14.223331253898937
7	31.468171092245516	28.82096069868996	18.169736092652364	21.54113211641216
8	28.883343730505302	32.412053486669016	16.458271176326996	22.246331606498686
9	25.58517996148526	13.097724375491607	16.108383737014837	45.2087119260083
10	15.88597466706447	24.888117388592043	29.33901109333044	29.886896851013045
11	40.939542705253736	19.44723209200141	18.858661748352272	20.75456345439258
12	24.836583579701106	22.15682551737232	27.06067427920475	25.945916623721825
13	30.293742710678345	17.469961214028046	23.192926306653288	29.04336976864032
14	23.651305975209524	17.714068729827225	23.39906154221704	35.23556375274621
15	26.523637744446553	25.36005858580379	20.499606715668993	27.616696954080666
16	26.73519759147251	25.66926143914942	21.400092218394857	26.195448750983214
17	24.380916216875967	26.358187094849335	23.469581491225693	25.791315197049013
18	26.1818872223277	23.949659605630746	24.81488513385229	25.053568038189265
19	26.355474789118226	24.606037592557435	23.86286582223548	25.175621796088855
20	26.64026689088394	23.201063223846592	23.3502400390572	26.808429846212267
21	27.462095527407847	23.556375274620954	23.087146383140308	25.894382814830884
22	25.84556131167105	23.65401828094063	24.283273210556295	26.217147196832023
23	24.698255987414903	23.827605847731157	25.140361821584527	26.333776343269417
24	24.48127152892674	24.679269847297185	24.08798719791695	26.751471425859126
25	25.064417261113675	23.984919580135074	24.10426103230356	26.846402126447693
26	25.18104640755106	24.315820879329518	24.283273210556295	26.219859502563132
27	25.539070764056525	23.96864574574846	25.069841872575875	25.42244161761914
28	25.256990968021913	23.984919580135074	25.65569991049391	25.1023895413491
29	25.35734628007269	24.2914101277496	24.421600802842498	25.929642789335215
30	25.189183324744363	24.459573083077924	24.817597439583388	25.53364615259432
31	26.127641107705664	24.367354688220455	24.052727223412624	25.45227698066126
32	26.040847324310395	24.50839458623776	23.569936803276466	25.880821286175376
33	24.497545363313353	24.220890178740948	24.513819197699966	26.767745260245736
34	25.379044725921506	24.296834739211803	24.408039274186986	25.916081260679704
35	26.40972090374027	23.789633567495727	24.326670102253924	25.473975426510076
36	24.35650546529605	24.890829694323145	24.2886978220185	26.46396701836231
37	26.442268572513495	23.933385771244133	24.47855922319564	25.145786433046734
38	25.44956467493016	23.90083810247091	25.015595757953836	25.634001464645095
39	25.89980742629309	24.231739401665354	23.515690688654427	26.352762483387128
40	26.076107298814723	25.12951259866012	23.724538229949278	25.069841872575875
41	24.76063901923025	24.44058694296021	25.107814152811304	25.69095988499824
42	25.799452114242317	25.539070764056525	24.123247172421276	24.538229949279884
43	25.50450254963654	23.730606488011283	25.013561896495606	25.75132906585657
44	25.534338721926876	24.65824020831073	24.077791038298795	25.7296300314636
45	25.465838509316768	24.961349643331797	24.055439529143722	25.51737231820771
46	24.719954433263716	24.79318668800347	24.32124549079172	26.16561338794109
47	25.140361821584527	24.22631479020315	24.934226586020777	25.699096802191544
48	26.3317782358685	25.561462514918087	23.801128349788435	24.305630899424976
49	25.25564868310413	24.827081129465377	24.108281118615565	25.80898906881493
50	26.024573489923785	24.64129756706176	24.106973338034663	25.227155604979796
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	1.5
4	1.0
5	3.0
6	5.0
7	4.5
8	4.0
9	5.0
10	6.0
11	11.0
12	16.0
13	19.5
14	23.0
15	27.5
16	32.0
17	48.5
18	65.0
19	73.0
20	81.0
21	109.0
22	137.0
23	164.5
24	192.0
25	246.5
26	301.0
27	353.5
28	406.0
29	463.5
30	521.0
31	553.0
32	585.0
33	671.0
34	757.0
35	853.5
36	950.0
37	1083.5
38	1217.0
39	1335.0
40	1453.0
41	1561.5
42	1670.0
43	1678.5
44	1687.0
45	1749.5
46	1812.0
47	2007.5
48	2203.0
49	2278.0
50	2353.0
51	2261.5
52	2170.0
53	2288.0
54	2406.0
55	2494.5
56	2583.0
57	2527.5
58	2472.0
59	2357.5
60	2243.0
61	2114.0
62	1985.0
63	1744.5
64	1504.0
65	1350.0
66	1196.0
67	1075.5
68	955.0
69	905.5
70	856.0
71	723.0
72	590.0
73	516.0
74	442.0
75	357.5
76	273.0
77	248.5
78	224.0
79	203.5
80	183.0
81	144.5
82	106.0
83	95.0
84	84.0
85	68.5
86	53.0
87	43.0
88	33.0
89	19.0
90	5.0
91	6.0
92	7.0
93	5.0
94	3.0
95	4.5
96	6.0
97	4.0
98	2.0
99	5.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00813691719330603
2	0.0027123057311020096
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0027123057311020096
44	0.0027123057311020096
45	0.0
46	0.0
47	0.0
48	0.0027123057311020096
49	0.005424611462204019
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	36869.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.25506523095282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.61962400206026	78.87927527190864
2	3.9821014679371625	6.7102443787463715
3	0.9625289724439866	2.432938240798503
4	0.40239505536955966	1.356152865551005
5	0.20602626834921456	0.8679378339526431
6	0.14808138037599794	0.7485963817841547
7	0.09657481328869431	0.569584203531422
8	0.09657481328869431	0.6509533754644824
9	0.06760236930208602	0.5126257831782799
>10	0.3991758949266031	6.064715614744094
>50	0.016095802214782386	0.8869239740703573
>100	0.0032191604429564775	0.3200520762700372
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	118	0.3200520762700372	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	81	0.2196967642192628	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	65	0.17629987252163062	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	62	0.1681629553283246	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	61	0.1654506495972226	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC	58	0.15731373240391658	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	50	0.1356152865551005	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT	49	0.13290298082399848	No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC	49	0.13290298082399848	No Hit
GGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTT	41	0.1112045349751824	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	41	0.1112045349751824	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC	39	0.10577992351297838	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.00271230573110201	0.0
32	0.0	0.0	0.0	0.00542461146220402	0.0
33	0.0	0.0	0.0	0.00542461146220402	0.0
34	0.0	0.0	0.0	0.00542461146220402	0.0
35	0.0	0.0	0.0	0.00813691719330603	0.0
36	0.0	0.0	0.0	0.00813691719330603	0.0
37	0.0	0.0	0.0	0.00813691719330603	0.0
38	0.0	0.0	0.0	0.013561528655510049	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGGC	20	7.7575806E-4	44.0	30
TCTAGCG	20	7.7575806E-4	44.0	28
TTTCACC	20	7.7575806E-4	44.0	21
AGAATTT	20	7.7575806E-4	44.0	17
GCAATAC	20	7.7575806E-4	44.0	37
CCTCTAG	20	7.7575806E-4	44.0	26
CACCTCT	20	7.7575806E-4	44.0	24
AGCGGCG	20	7.7575806E-4	44.0	31
ACCTCTA	20	7.7575806E-4	44.0	25
CTAGCGG	20	7.7575806E-4	44.0	29
CTTGCGC	20	7.7575806E-4	44.0	3
GCGCAAT	20	7.7575806E-4	44.0	35
CGCAATA	20	7.7575806E-4	44.0	36
CGCCGGT	25	4.3592616E-5	44.0	7
AATTTCA	25	0.0023234098	35.2	19
TCGGAAC	25	0.0023234098	35.2	40
CGGAACT	25	0.0023234098	35.2	41
TCTTGCG	25	0.0023234098	35.2	2
AATACGA	25	0.0023234098	35.2	39
CGGTCCA	25	0.0023234098	35.2	10
>>END_MODULE