FastQCFastQC Report
Thu 2 Feb 2017
SRR4062216_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062216_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2190985
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40240.1836616864104501No Hit
TATCAACGCAGAGTACTTTTTTTTT23250.10611665529430828No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGC456.7680835E-414.77656817
GGTATCA6700.013.8886721
GTATCAA20700.013.6237141
CATTCGT500.001497971813.3022579
TGGACCG1900.012.5015495
TCGCGCT550.003065236112.0929619
CCGTGCG658.0143E-411.6942929
ACCGAGC1408.3346094E-910.8589858
CTTAGAC1502.1373125E-910.765543
TAGATTG1158.806801E-710.7404615
GTTCTAG909.565932E-510.5503511
TTAGATT1002.3981342E-510.4505794
GTATAGG1752.382876E-1010.30921
GTATTAG1054.1286003E-59.9474741
TTAGACT1554.0614395E-89.8069944
TATCAAC29800.09.5930982
GGACCGC1701.6605554E-89.5016136
GCGATTG800.0045171869.49987316
ATTAGCC1002.758889E-49.4990053
CTTACAC1901.0531949E-99.4990053
Produced by FastQC (version 0.11.5)