##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062216_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2190985
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.308834154501287	32.0	32.0	32.0	32.0	32.0
2	30.906815884179945	32.0	32.0	32.0	32.0	32.0
3	30.938465119569507	32.0	32.0	32.0	32.0	32.0
4	30.94097129829734	32.0	32.0	32.0	32.0	32.0
5	30.872341435473086	32.0	32.0	32.0	32.0	32.0
6	34.526068412152526	36.0	36.0	36.0	32.0	36.0
7	34.47612192689589	36.0	36.0	36.0	32.0	36.0
8	34.43263554976415	36.0	36.0	36.0	32.0	36.0
9	34.55624342476101	36.0	36.0	36.0	32.0	36.0
10	34.28811014224196	36.0	36.0	36.0	32.0	36.0
11	34.52994977144983	36.0	36.0	36.0	32.0	36.0
12	34.39481374815437	36.0	36.0	36.0	32.0	36.0
13	34.45470461915531	36.0	36.0	36.0	32.0	36.0
14	34.38268221827169	36.0	36.0	36.0	32.0	36.0
15	34.34351079537286	36.0	36.0	36.0	32.0	36.0
16	34.34239485893331	36.0	36.0	36.0	32.0	36.0
17	34.29671312218021	36.0	36.0	36.0	32.0	36.0
18	34.29376239453944	36.0	36.0	36.0	32.0	36.0
19	34.297662923297054	36.0	36.0	36.0	32.0	36.0
20	34.276175783951054	36.0	36.0	36.0	32.0	36.0
21	34.27148885090496	36.0	36.0	36.0	32.0	36.0
22	34.243023571589944	36.0	36.0	36.0	32.0	36.0
23	34.207313605524455	36.0	36.0	36.0	32.0	36.0
24	34.196957076383455	36.0	36.0	36.0	32.0	36.0
25	33.84766349381671	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	27.0
5	167.0
6	461.0
7	91.0
8	389.0
9	321.0
10	155.0
11	34.0
12	95.0
13	99.0
14	296.0
15	499.0
16	810.0
17	1071.0
18	1487.0
19	1849.0
20	2696.0
21	3633.0
22	5236.0
23	7790.0
24	10889.0
25	15651.0
26	21685.0
27	28420.0
28	38149.0
29	51698.0
30	68485.0
31	94428.0
32	136167.0
33	195621.0
34	447746.0
35	1054839.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.261902979692955	16.735738241249056	12.081668160681783	25.920690618376202
2	16.650197651008703	18.837800202009785	37.604445128054394	26.90755701892712
3	19.464037455487833	22.13111611548106	28.09987704098725	30.304969388043858
4	13.49387955199334	15.101852270273799	34.25218792835947	37.15208024937339
5	15.228920745454596	35.85347501682469	33.56253258784221	15.355071649878507
6	35.655478468888596	33.9383604029779	16.1477055844472	14.25845554368631
7	31.004716175082404	30.0411031883756	19.731952017647505	19.22222861889449
8	28.18063518579102	33.62368796685352	18.56151089195408	19.634165955401382
9	26.883187149334336	15.121475750089793	18.122703165598733	39.87263393497714
10	16.03953726030753	27.37795770772738	31.55243207075647	25.03007296120862
11	36.63626274980849	21.61146244119868	21.874960006874247	19.877314802118583
12	24.46257739817809	23.28654555957946	28.74559650755289	23.505280534689557
13	29.533274073803252	20.047575196002036	24.739924546927377	25.679226183267335
14	23.175364177766365	19.118470240899494	25.277014544263153	32.42915103707099
15	24.90815939452384	27.09482959557227	22.192108883333407	25.80490212657048
16	25.41081743072311	26.369315273817357	23.31802478613731	24.90184250932222
17	24.204576404718253	26.225261256847883	24.990311972756537	24.57985036567733
18	24.452925127358746	26.049176080635945	25.418425288884684	24.079473503120628
19	25.475735784577992	25.424886468508145	25.207372008662198	23.89200573825166
20	25.536073153650168	25.170049069704014	25.131674103339215	24.1622036733066
21	25.41891491226988	24.817296495226664	25.203923928094518	24.55986466440894
22	25.519995942832313	24.480483789833233	25.13863131813632	24.860888949198138
23	24.864446590357826	24.807804222049047	25.209736640394965	25.118012547198166
24	24.701425273745844	24.929796165616636	25.289141121698982	25.07963743893854
25	24.92384747933786	24.903884281059906	25.24449207593507	24.92777616366716
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	147.0
1	147.0
2	374.5
3	602.0
4	602.0
5	602.0
6	1266.5
7	1931.0
8	1931.0
9	1931.0
10	2477.5
11	3024.0
12	3024.0
13	3024.0
14	4405.0
15	5786.0
16	5786.0
17	5786.0
18	9724.5
19	13663.0
20	13663.0
21	13663.0
22	20669.5
23	27676.0
24	27676.0
25	27676.0
26	39462.5
27	51249.0
28	51249.0
29	51249.0
30	68315.0
31	85381.0
32	85381.0
33	85381.0
34	105540.5
35	125700.0
36	125700.0
37	125700.0
38	152444.0
39	179188.0
40	179188.0
41	179188.0
42	207172.0
43	235156.0
44	235156.0
45	235156.0
46	259193.0
47	283230.0
48	283230.0
49	283230.0
50	296572.0
51	309914.0
52	309914.0
53	309914.0
54	298107.0
55	286300.0
56	286300.0
57	286300.0
58	259356.0
59	232412.0
60	232412.0
61	232412.0
62	198534.0
63	164656.0
64	164656.0
65	164656.0
66	131637.5
67	98619.0
68	98619.0
69	98619.0
70	74120.5
71	49622.0
72	49622.0
73	49622.0
74	35784.5
75	21947.0
76	21947.0
77	21947.0
78	15441.0
79	8935.0
80	8935.0
81	8935.0
82	6082.5
83	3230.0
84	3230.0
85	3230.0
86	2202.0
87	1174.0
88	1174.0
89	1174.0
90	785.0
91	396.0
92	396.0
93	396.0
94	288.0
95	180.0
96	180.0
97	180.0
98	523.5
99	867.0
100	867.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.58473015561494E-4
2	9.12831443391899E-5
3	5.020572938655445E-4
4	0.009767296444293319
5	0.034276820699365815
6	0.0632592190270586
7	0.10712076988203935
8	0.1094028484905191
9	0.12081324153291784
10	0.1356923940602058
11	0.1418996478752707
12	0.13459699632813552
13	0.13099131212673754
14	0.11693370789850226
15	0.13487084576115307
16	0.12163478983197056
17	0.12400815158478949
18	0.11328238212493467
19	0.09881400374717308
20	0.09393035552502642
21	0.09383907238068723
22	0.10342380253630216
23	0.08265688719913646
24	0.0913744274835291
25	0.08932055673589732
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2190985.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.98982902139693
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.557653674078	37.554058289930126
2	15.951961642585143	17.224873632781083
3	6.141543785327067	9.947426966916938
4	2.8711626590423154	6.200543242172559
5	1.618480081091286	4.369073142632758
6	0.9920678998417811	3.213694577404446
7	0.6254000847283862	2.363567055111691
8	0.4498979988221215	1.9431932826779983
9	0.3053553473967646	1.4837474697048525
>10	1.4380895717448654	12.812669818268086
>50	0.038436009271496284	1.371822176958328
>100	0.00884713972005958	0.8291216311689907
>500	7.643813197809131E-4	0.2712736888496504
>1k	3.3972503101359266E-4	0.41493502542252664
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4024	0.1836616864104501	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2325	0.10611665529430828	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.302651547135192E-4
2	0.0	0.0	0.0	0.0	7.302651547135192E-4
3	0.0	0.0	0.0	0.0	7.302651547135192E-4
4	0.0	0.0	0.0	0.0	8.671898712223041E-4
5	0.0	4.564157216959495E-5	0.0	0.0	9.128314433918991E-4
6	0.0	4.564157216959495E-5	0.0	0.0	0.001004114587731089
7	0.0	9.12831443391899E-5	0.0	0.0	0.0010953977320702789
8	0.0	9.12831443391899E-5	0.0	0.0	0.0010953977320702789
9	0.0	9.12831443391899E-5	0.0	0.0	0.0010953977320702789
10	0.0	9.12831443391899E-5	0.0	0.0	0.0010953977320702789
11	0.0	9.12831443391899E-5	0.0	0.0	0.0013692471650878486
12	0.0	9.12831443391899E-5	0.0	0.0	0.002282078608479748
13	0.0	9.12831443391899E-5	0.0	0.0	0.0025102864693277225
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTGC	45	6.7680835E-4	14.776568	17
GGTATCA	670	0.0	13.888672	1
GTATCAA	2070	0.0	13.623714	1
CATTCGT	50	0.0014979718	13.302257	9
TGGACCG	190	0.0	12.501549	5
TCGCGCT	55	0.0030652361	12.092961	9
CCGTGCG	65	8.0143E-4	11.694292	9
ACCGAGC	140	8.3346094E-9	10.858985	8
CTTAGAC	150	2.1373125E-9	10.76554	3
TAGATTG	115	8.806801E-7	10.740461	5
GTTCTAG	90	9.565932E-5	10.550351	1
TTAGATT	100	2.3981342E-5	10.450579	4
GTATAGG	175	2.382876E-10	10.3092	1
GTATTAG	105	4.1286003E-5	9.947474	1
TTAGACT	155	4.0614395E-8	9.806994	4
TATCAAC	2980	0.0	9.593098	2
GGACCGC	170	1.6605554E-8	9.501613	6
GCGATTG	80	0.004517186	9.499873	16
ATTAGCC	100	2.758889E-4	9.499005	3
CTTACAC	190	1.0531949E-9	9.499005	3
>>END_MODULE