FastQCFastQC Report
Thu 2 Feb 2017
SRR4062216_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062216_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2190985
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8700.027.0681691
GTATCAA20850.026.283771
TCGATCG1051.791961E-618.85943241
TATCAAC31200.017.2749792
TCAACGC32950.016.2235894
GTATTAG1906.148184E-1016.2169361
ATCAACG33350.016.0949673
TTAGGAC2500.015.8390313
CAACGCA34100.015.6764595
AACGCAG35650.014.9948746
GTATAGG2509.094947E-1214.9659161
GTACGGG1655.398506E-714.6724651
ATCGATC1503.249137E-614.66844740
CGCGAAG900.004360314.66677331
GTATTAT2900.014.4194931
TCTATAC2803.6379788E-1214.1419933
CTATACT3650.013.8621654
TAGGACG1302.333074E-413.5376334
GTATATG3101.8189894E-1213.4892021
TAGGACA4650.013.2465014
Produced by FastQC (version 0.11.5)