FastQCFastQC Report
Thu 2 Feb 2017
SRR4062215_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062215_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332564
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGGGTCTTCTCGTCTTATTAT14890.4477333686147628No Hit
GATATACACTGTTCTACAAATCCCG11250.3382807519755596No Hit
GTATCAACGCAGAGTACTTTTTTTT10170.3058057997859059No Hit
GTATTAGAGGCACTGCCTGCCCAGT8640.25979961751722974No Hit
TTGTAGAACAGTGTATATCAATGAG8440.2537857374821087No Hit
CTGTAGGACGTGGAATATGGCAAGA8440.2537857374821087No Hit
GTCCTAAAGTGTGTATTTCTCATTT8110.24386283542415896No Hit
GTGTATATCAATGAGTTACAATGAG7910.23784895538903789No Hit
GTCCTACAGTGGACATTTCTAAATT7680.23093299334864867No Hit
GTGTATATCAATGAGTTACAATGAA7600.22852744133460026No Hit
GTCAGGATACCGCGGCCGTTAAACT7420.22311494930299128No Hit
CCATTGGGATGTCCTGATCCAACAT7300.21950662128191867No Hit
CTTTAGGACGTGAAATATGGCGAGG6780.20387053319060391No Hit
ATAAATAATCCACCTATAACTTCTC6740.2026677571835797No Hit
GTTATATAATTTAAGCTCCATAGGG6740.2026677571835797No Hit
CTTCTACACCATTGGGATGTCCTGA6680.20086359317304336No Hit
GACTATAGGCAATAATCACACTATA6590.19815734715723893No Hit
TATCAACGCAGAGTACTTTTTTTTT6530.1963531831467026No Hit
CTGTTAGTATGAGTAACAAGAATTC6490.19515040713967838No Hit
ACCTATAACTTCTCTGTTAACCCAA6450.19394763113265417No Hit
CTCTAATACTTGTAATGCTAGAGGT6090.18312264706943626No Hit
GTTCATGCTAGTCCCTAATTAAGGA6080.1828219530676802No Hit
CCTATAACTTCTCTGTTAACCCAAC5950.1789129310448515No Hit
ATCTATAACTTTATAGATGCAACAC5910.1777101550378273No Hit
ATCCTGACCGTGCAAAGGTAGCATA5770.17350043901324255No Hit
GGTCAGGATACCGCGGCCGTTAAAC5640.16959141699041386No Hit
CACTATAAATAATCCACCTATAACT5620.16899002898690177No Hit
GTCCTACAGTGTGCATTTCTCATTT5560.16718586497636545No Hit
GCCTAAAGGAAAGATCCAAAAAGAT5490.16508100696407307No Hit
CTGTTAACCCAACACCGGAATGCCT5270.15846573892543991No Hit
GTATCAACGCAGAGTACATGGGAGA5180.15575949290963542No Hit
CCTCTAGCATTACAAGTATTAGAGG5030.15124908288329464No Hit
GTACATGGGAGAAATCGTAAATAGA5000.1503470008780265No Hit
GTTCTACAGTGTGGTTTTTATCATT5000.1503470008780265No Hit
GATTAAAGATAAGAGACAGTTGGAC4940.14854283686749015No Hit
GTATCCTGACCGTGCAAAGGTAGCA4810.14463381484466148No Hit
GATATACACTGTTCTACAATGCCGG4770.14343103883763725No Hit
GTTCTACAAATCCCGTTTCCAACGA4750.14282965083412516No Hit
ATCGTAAATAGATAGAAACCGACCT4660.1401234048183207No Hit
GGTATCAACGCAGAGTACTTTTTTT4490.1350116067884678No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA4440.1335081367796875No Hit
GATTAAACCTTGTACCTTTTGCATA4280.12869703275159067No Hit
GACCTGGATTGCTCCGGTCTGAACT4090.12298384671822567No Hit
GTTAGTATGAGTAACAAGAATTCCA4040.12148037670944539No Hit
GATATATTTTGATCAACGGACCAAG4010.12057829470417723No Hit
ACCTTTGCACGGTCAGGATACCGCG3930.11817274269012883No Hit
GGATTGCTCCGGTCTGAACTCAGAT3820.11486510867081223No Hit
CTATAGAACTAGTACCGCAAGGGAA3800.11426372066730013No Hit
ATTATAACCTAGACTTACAAGTCAA3750.11276025065851986No Hit
GATAGAAACCGACCTGGATTGCTCC3730.11215886265500777No Hit
CATTTACACCTACTACCCAACTATC3730.11215886265500777No Hit
GGATACCGCGGCCGTTAAACTTTAG3650.10975331064095933No Hit
CTGTAGGACCTGGAATATGGCGAGA3640.10945261663920328No Hit
GTATAGGGGTCCTAGGAAGATAATA3570.10734775862691091No Hit
GTCCTGATCCAACATCGAGGTCGTA3540.10644567662164274No Hit
GTATTGGAATTAGTGAAATTGGAGT3460.10404012460759433No Hit
GTTATAGATTAACCCAATTTTAAGT3380.1016345725935459No Hit
CTATAAATAATCCACCTATAACTTC3340.10043179658652168No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAG250.00601403819.0045195
CTGGGGG307.700736E-419.00165710
TTAGAAC250.00602976118.9959343
AGCCTAT402.7479243E-416.6289566
TCCGGTG402.7510856E-416.62645112
GGAATTT402.757418E-416.62144319
ACGGACC702.4796464E-816.28713416
TAGGATG350.002166285416.2846814
CGGACCA702.4841938E-816.28468117
GTTATGG350.002168466816.282233
TAGAAAT601.4593115E-615.832334
CTACACT601.4593115E-615.832334
GGACCAA851.0095391E-915.64606718
TTAGGCA551.1236603E-515.5444694
AATAGAC508.663482E-515.2036157
CGGTTTC1250.015.20132513
CTAATCT508.6857865E-515.1990364
CCAACGA1004.0017767E-1115.19674719
GGTGGCT953.0013325E-1015.0035698
GTTTAGA704.4388798E-714.9186361
Produced by FastQC (version 0.11.5)