FastQCFastQC Report
Thu 2 Feb 2017
SRR4062214_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062214_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2729528
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61370.22483740778625463No Hit
TATCAACGCAGAGTACTTTTTTTTT38100.13958457286387976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21050.013.535011
CGACTTT2500.012.16025813
CCGTCGT4350.012.0132919
GTATCAA53900.011.6995381
CGTCGTA4550.011.69405710
AGGCGTA658.0192223E-411.69362812
CGTCTTA1406.82121E-1011.53532415
ACCGTCG4900.011.2461698
GTCGAAT700.001490164710.85876811
TACCGTC5100.010.8053417
CGCGTAA1605.4387783E-1010.689110
CGTGCGC909.505759E-510.55713610
TTTCGTT2800.010.51634417
GCATCGC2900.010.48298114
ATACCGT5450.010.4598976
GCGTAAC1659.276846E-1010.36518811
CGAGCCG4600.010.32573815
CTTTCGT2950.010.304358516
CGCATCG2900.010.15538813
GTCGTAG5350.010.12301111
Produced by FastQC (version 0.11.5)