##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062214_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2729528
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.222135109073804	32.0	32.0	32.0	32.0	32.0
2	30.71354424647778	32.0	32.0	32.0	32.0	32.0
3	30.752362313191146	32.0	32.0	32.0	32.0	32.0
4	30.754818781855324	32.0	32.0	32.0	32.0	32.0
5	30.65853033931141	32.0	32.0	32.0	32.0	32.0
6	34.31548641376824	36.0	36.0	36.0	32.0	36.0
7	34.229604165995	36.0	36.0	36.0	32.0	36.0
8	34.20758973712671	36.0	36.0	36.0	32.0	36.0
9	34.33651202698782	36.0	36.0	36.0	32.0	36.0
10	33.990567599966	36.0	36.0	36.0	32.0	36.0
11	34.342580475452166	36.0	36.0	36.0	32.0	36.0
12	34.13204334229215	36.0	36.0	36.0	32.0	36.0
13	34.228985011327964	36.0	36.0	36.0	32.0	36.0
14	34.13453754641828	36.0	36.0	36.0	32.0	36.0
15	34.08259193530896	36.0	36.0	36.0	32.0	36.0
16	34.08146353508739	36.0	36.0	36.0	32.0	36.0
17	34.017153881550215	36.0	36.0	36.0	32.0	36.0
18	34.02206901706082	36.0	36.0	36.0	32.0	36.0
19	34.02448408662597	36.0	36.0	36.0	32.0	36.0
20	34.00180800490048	36.0	36.0	36.0	32.0	36.0
21	33.98644820642983	36.0	36.0	36.0	32.0	36.0
22	33.96056424407443	36.0	36.0	36.0	32.0	36.0
23	33.92052728530354	36.0	36.0	36.0	32.0	36.0
24	33.89327971722584	36.0	36.0	36.0	32.0	36.0
25	33.53693678907123	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	46.0
5	214.0
6	569.0
7	156.0
8	517.0
9	378.0
10	212.0
11	51.0
12	119.0
13	122.0
14	426.0
15	655.0
16	957.0
17	1454.0
18	1988.0
19	2608.0
20	4087.0
21	5941.0
22	8735.0
23	12638.0
24	17633.0
25	24553.0
26	33652.0
27	42738.0
28	56627.0
29	74991.0
30	98320.0
31	133174.0
32	189085.0
33	268660.0
34	576682.0
35	1171539.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.89218746164586	19.672965385197347	14.242734100825169	24.19211305233162
2	15.631863612699812	21.31450902923993	37.77215855822322	25.281468799837043
3	17.748714424095702	25.230004147255407	29.976523310359788	27.044758118289103
4	13.055413371653982	17.729296426015782	36.944385107855595	32.27090509447464
5	14.856847785375393	36.785732348127304	32.93582063406085	15.421599232436451
6	31.0810696865325	36.62277767288738	18.348911084919063	13.947241555661058
7	28.748361929499428	32.12147597074818	21.07128068597056	18.058881413781837
8	27.779147062663856	32.45520604558838	20.193773372592688	19.571873519155073
9	27.263363617156138	16.054130866023428	20.181989048524542	36.50051646829589
10	16.84076641553845	27.612780244710606	30.676358631052832	24.870094708698115
11	34.533842961380195	23.089371775078806	23.114393347930474	19.26239191561053
12	24.53795366825112	24.65820916164899	28.35751466599752	22.44632250410237
13	27.903881624609493	21.205390224110886	25.761269741582087	25.12945840969753
14	23.809513329518612	20.929618381090716	26.01258858224394	29.248279707146736
15	24.709216049036236	27.635013450993295	23.14392617857152	24.51184432139895
16	24.76509427041303	27.219426307681022	24.196463942484044	23.819015479421907
17	23.249381716111035	26.90494164911408	25.498900624850062	24.346776009924824
18	23.657908037696902	26.593561127620603	26.44372863924225	23.30480219544025
19	24.864647996775684	25.954321930746865	26.28738889363604	22.89364117884141
20	25.38875589313008	25.17532703754881	25.69932788246304	23.736589186858062
21	26.36651373472132	25.107511365382422	25.215912012119162	23.310062887777093
22	25.31890641377626	25.062255607772432	25.620263897762175	23.998574080689135
23	24.06313148628427	25.204449843557324	25.780719177969175	24.951699492189235
24	23.837267933058083	25.927103467926383	26.038839572696492	24.196789026319042
25	24.297656189265027	25.41114597222161	25.976159987561477	24.315037850951885
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	761.0
1	761.0
2	959.5
3	1158.0
4	1158.0
5	1158.0
6	2292.0
7	3426.0
8	3426.0
9	3426.0
10	4299.5
11	5173.0
12	5173.0
13	5173.0
14	7823.5
15	10474.0
16	10474.0
17	10474.0
18	18283.0
19	26092.0
20	26092.0
21	26092.0
22	40396.0
23	54700.0
24	54700.0
25	54700.0
26	77827.5
27	100955.0
28	100955.0
29	100955.0
30	124081.0
31	147207.0
32	147207.0
33	147207.0
34	175706.0
35	204205.0
36	204205.0
37	204205.0
38	229656.0
39	255107.0
40	255107.0
41	255107.0
42	283603.5
43	312100.0
44	312100.0
45	312100.0
46	334839.5
47	357579.0
48	357579.0
49	357579.0
50	357265.0
51	356951.0
52	356951.0
53	356951.0
54	323357.0
55	289763.0
56	289763.0
57	289763.0
58	259642.5
59	229522.0
60	229522.0
61	229522.0
62	197376.0
63	165230.0
64	165230.0
65	165230.0
66	133333.5
67	101437.0
68	101437.0
69	101437.0
70	77200.0
71	52963.0
72	52963.0
73	52963.0
74	39754.0
75	26545.0
76	26545.0
77	26545.0
78	21142.0
79	15739.0
80	15739.0
81	15739.0
82	11188.0
83	6637.0
84	6637.0
85	6637.0
86	4894.5
87	3152.0
88	3152.0
89	3152.0
90	2204.5
91	1257.0
92	1257.0
93	1257.0
94	820.0
95	383.0
96	383.0
97	383.0
98	697.5
99	1012.0
100	1012.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011357274957428539
2	2.5645459581290247E-4
3	4.3963644996497563E-4
4	0.010148274720024853
5	0.03275291552239068
6	0.06642174031554172
7	0.10979920337875267
8	0.11152111280778214
9	0.12163275115697658
10	0.13540802658921247
11	0.14214911882200879
12	0.13427229909346966
13	0.13027893467295446
14	0.11899493245718674
15	0.13581102666834705
16	0.12192584212361991
17	0.12580929743164387
18	0.1154045681158061
19	0.10071338341280983
20	0.096133837059008
21	0.09551101875489096
22	0.10499983879996835
23	0.08539938040569652
24	0.09404556392167437
25	0.09217710900932322
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2729528.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.64895817633753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.27668845796823	34.67536635739536
2	15.974194616999432	15.54255851646163
3	5.687401723311374	8.30058505708215
4	2.5427276619026378	4.948042067108718
5	1.3261438281603495	3.2257757815990495
6	0.7778456972000138	2.270482967443655
7	0.49807060552177174	1.6961431239824247
8	0.3356742817155911	1.3064163273643148
9	0.2357464219559463	1.0321936039760446
>10	1.1695918828802938	10.307462404924571
>50	0.09180449940970552	3.1249731931264924
>100	0.07036078838662152	7.2497726283392625
>500	0.01027437749416853	3.5265759205236327
>1k	0.003399610200266325	2.568100261021424
>5k	7.554689333925168E-5	0.2255517896513557
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6137	0.22483740778625463	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3810	0.13958457286387976	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1981822498248781E-4
2	0.0	0.0	0.0	0.0	2.1981822498248781E-4
3	0.0	0.0	0.0	0.0	2.1981822498248781E-4
4	0.0	0.0	0.0	3.6636370830414634E-5	2.1981822498248781E-4
5	0.0	0.0	0.0	3.6636370830414634E-5	2.5645459581290247E-4
6	0.0	0.0	0.0	3.6636370830414634E-5	2.9309096664331707E-4
7	0.0	0.0	0.0	3.6636370830414634E-5	2.9309096664331707E-4
8	0.0	0.0	0.0	7.327274166082927E-5	3.297273374737317E-4
9	0.0	0.0	0.0	1.0990911249124391E-4	3.663637083041464E-4
10	0.0	0.0	0.0	1.0990911249124391E-4	4.03000079134561E-4
11	0.0	0.0	0.0	1.4654548332165853E-4	4.03000079134561E-4
12	0.0	0.0	0.0	1.831818541520732E-4	4.3963644996497563E-4
13	0.0	0.0	0.0	1.831818541520732E-4	5.129091916258049E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2105	0.0	13.53501	1
CGACTTT	250	0.0	12.160258	13
CCGTCGT	435	0.0	12.013291	9
GTATCAA	5390	0.0	11.699538	1
CGTCGTA	455	0.0	11.694057	10
AGGCGTA	65	8.0192223E-4	11.693628	12
CGTCTTA	140	6.82121E-10	11.535324	15
ACCGTCG	490	0.0	11.246169	8
GTCGAAT	70	0.0014901647	10.858768	11
TACCGTC	510	0.0	10.805341	7
CGCGTAA	160	5.4387783E-10	10.6891	10
CGTGCGC	90	9.505759E-5	10.557136	10
TTTCGTT	280	0.0	10.516344	17
GCATCGC	290	0.0	10.482981	14
ATACCGT	545	0.0	10.459897	6
GCGTAAC	165	9.276846E-10	10.365188	11
CGAGCCG	460	0.0	10.325738	15
CTTTCGT	295	0.0	10.3043585	16
CGCATCG	290	0.0	10.155388	13
GTCGTAG	535	0.0	10.123011	11
>>END_MODULE