FastQCFastQC Report
Thu 2 Feb 2017
SRR4062214_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062214_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2729528
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA27440.10053020155865776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22400.026.329861
GTATCAA53250.025.8298681
CGTCTTA2950.017.89780415
TCAACGC81750.016.8995254
GATATAC4450.016.8143711
ATCAACG82100.016.8009913
AACGCAG83300.016.637586
CAACGCA83450.016.5813145
TATCAAC83650.016.5428832
TAAACGC6600.016.0001428
GTAAACG6950.015.51064227
CGCTTCG6850.015.41591232
AACGAAT3600.015.27763131
AACGCTT7100.015.18323230
TCTATAC4700.014.9783073
TTTATAC4450.014.8310463
CAACGAA4100.014.48793130
AAACGCT7450.014.46992529
TCCAACG4300.014.325706528
CGCAATA4500.014.17790236
Produced by FastQC (version 0.11.5)