##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062213_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10335
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.360328979196904	32.0	21.0	32.0	14.0	32.0
2	25.248379293662314	32.0	14.0	32.0	14.0	32.0
3	25.516207063376875	32.0	14.0	32.0	14.0	32.0
4	25.157039187227866	32.0	14.0	32.0	14.0	32.0
5	25.597774552491533	32.0	21.0	32.0	14.0	32.0
6	29.023028543783262	32.0	21.0	36.0	14.0	36.0
7	28.67431059506531	32.0	21.0	36.0	14.0	36.0
8	28.344267053701017	32.0	21.0	36.0	14.0	36.0
9	28.33952588292211	32.0	21.0	36.0	14.0	36.0
10	27.185002418964682	32.0	14.0	36.0	14.0	36.0
11	29.32694726656991	36.0	21.0	36.0	14.0	36.0
12	28.07489114658926	32.0	21.0	36.0	14.0	36.0
13	28.49230769230769	32.0	21.0	36.0	14.0	36.0
14	27.85312046444122	32.0	21.0	36.0	14.0	36.0
15	27.973971940009676	32.0	21.0	36.0	14.0	36.0
16	28.098209966134494	32.0	21.0	36.0	14.0	36.0
17	27.772907595549103	32.0	21.0	36.0	14.0	36.0
18	27.907014997581037	32.0	21.0	36.0	14.0	36.0
19	27.717851959361393	32.0	21.0	36.0	14.0	36.0
20	27.81635220125786	32.0	21.0	36.0	14.0	36.0
21	27.62264150943396	32.0	14.0	36.0	14.0	36.0
22	27.54649250120948	32.0	14.0	36.0	14.0	36.0
23	27.42360909530721	32.0	14.0	36.0	14.0	36.0
24	27.313497822931787	32.0	14.0	36.0	14.0	36.0
25	26.463957426221576	32.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	2.0
7	0.0
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	12.0
15	47.0
16	120.0
17	228.0
18	331.0
19	498.0
20	533.0
21	521.0
22	550.0
23	543.0
24	473.0
25	482.0
26	440.0
27	439.0
28	414.0
29	473.0
30	468.0
31	544.0
32	589.0
33	673.0
34	987.0
35	966.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.27431059506531	19.177552007740687	12.607643928398646	28.940493468795353
2	15.936139332365748	18.355104015481373	38.75181422351234	26.95694242864054
3	18.635703918722786	22.148040638606677	28.243831640058055	30.972423802612482
4	12.483065608670408	15.057093090768337	35.620282562415326	36.839558738145925
5	14.981128423497534	35.88502854930804	33.949482241362624	15.184360785831801
6	34.18505613627565	35.53039101819589	15.602013162988774	14.682539682539684
7	29.99031945788964	29.72894482090997	19.961277831558565	20.31945788964182
8	27.466356859328105	33.71091102720496	18.143092264498016	20.67963984896892
9	25.680255640553888	14.147380652658082	18.572673574126078	41.599690132661955
10	15.046475600309837	27.352827265685516	32.45546088303641	25.14523625096824
11	37.07397366382649	20.94306738962045	21.446553059643687	20.536405886909375
12	23.6974627154755	23.34882820065853	28.985086190199493	23.96862289366647
13	29.37645236250968	19.44229279628195	24.41905499612703	26.76219984508133
14	22.62561719430729	18.34640333042889	26.54661632297415	32.481363152289674
15	23.998063891577928	27.06679574056147	23.65924491771539	25.275895450145207
16	25.04841208365608	26.055383423702555	23.450813323005423	25.44539116963594
17	22.84082106893881	26.161890007745935	26.074748257164988	24.922540666150272
18	23.535676251331203	25.317068448058865	26.866105140865525	24.281150159744406
19	24.336882865440465	25.06292352371733	26.2729912875121	24.32720232333011
20	23.361409623390454	24.87172039887695	26.527253364314067	25.23961661341853
21	23.82381413359148	24.598257502420136	26.611810261374636	24.966118102613745
22	24.3489205150547	23.603446606641494	27.05005324813631	24.99757963016749
23	23.204259438528556	24.453049370764763	26.282671829622462	26.06001936108422
24	23.356887039008807	24.518439647662376	27.170651437421355	24.95402187590746
25	23.552758954501453	24.288480154888674	26.85382381413359	25.304937076476282
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	3.5
3	5.0
4	5.0
5	5.0
6	13.0
7	21.0
8	21.0
9	21.0
10	21.0
11	21.0
12	21.0
13	21.0
14	39.5
15	58.0
16	58.0
17	58.0
18	71.0
19	84.0
20	84.0
21	84.0
22	144.0
23	204.0
24	204.0
25	204.0
26	275.5
27	347.0
28	347.0
29	347.0
30	444.0
31	541.0
32	541.0
33	541.0
34	610.0
35	679.0
36	679.0
37	679.0
38	734.5
39	790.0
40	790.0
41	790.0
42	904.0
43	1018.0
44	1018.0
45	1018.0
46	1133.0
47	1248.0
48	1248.0
49	1248.0
50	1260.0
51	1272.0
52	1272.0
53	1272.0
54	1234.5
55	1197.0
56	1197.0
57	1197.0
58	1098.0
59	999.0
60	999.0
61	999.0
62	884.0
63	769.0
64	769.0
65	769.0
66	641.0
67	513.0
68	513.0
69	513.0
70	392.0
71	271.0
72	271.0
73	271.0
74	218.5
75	166.0
76	166.0
77	166.0
78	118.0
79	70.0
80	70.0
81	70.0
82	50.5
83	31.0
84	31.0
85	31.0
86	22.5
87	14.0
88	14.0
89	14.0
90	8.5
91	3.0
92	3.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	6.0
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.009675858732462506
5	0.019351717464925013
6	0.029027576197387515
7	0.04837929366231253
8	0.05805515239477503
9	0.07740686985970005
10	0.06773101112723753
11	0.06773101112723753
12	0.08708272859216255
13	0.06773101112723753
14	0.05805515239477503
15	0.04837929366231253
16	0.06773101112723753
17	0.06773101112723753
18	0.05805515239477503
19	0.04837929366231253
20	0.05805515239477503
21	0.04837929366231253
22	0.05805515239477503
23	0.04837929366231253
24	0.038703434929850025
25	0.04837929366231253
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	10335.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.0880503144654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.11271081859317	90.43057571359458
2	3.187988482106129	5.999032414126754
3	0.462772521596051	1.3062409288824384
4	0.0925545043192102	0.3483309143686502
5	0.07198683669271905	0.3386550556361877
6	0.010283833813245578	0.05805515239477503
7	0.0	0.0
8	0.010283833813245578	0.07740686985970005
9	0.0	0.0
>10	0.030851501439736733	0.35800677310111273
>50	0.020567667626491155	1.0836961780358005
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	58	0.5611998064828254	No Hit
TATCAACGCAGAGTACTTTTTTTTT	54	0.5224963715529753	No Hit
GGTATCAACGCAGAGTACTTTTTTT	16	0.1548137397194001	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	11	0.10643444605708757	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE