FastQCFastQC Report
Thu 2 Feb 2017
SRR4062212_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062212_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2302752
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39110.1698402606967663No Hit
TATCAACGCAGAGTACTTTTTTTTT24840.10787093008713053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8100.016.2943761
GTATCAA23650.012.8477411
TTAGTAC701.0926339E-412.2127353
CGTCGTA1209.998985E-911.87684910
ACGGTAT1209.998985E-911.8768499
TTCTACG855.3344593E-511.1745652
GTCTAGG1658.185452E-1110.9339631
CTAAGAC1408.374627E-910.8557643
GTAGGAC2100.010.85576253
CTAGGAC2650.010.7533513
TAGGACA2750.010.7084284
CAAGACG2850.010.6660074
TAAGACA2050.010.6578784
TACGCTA909.5037016E-510.5571999
GTAATAC1355.7014404E-810.55421453
GCGTTAT1002.4129338E-510.4448121
CGTTATT1106.060449E-610.361872
TCTAGGA2053.6379788E-1210.1933832
GTCTTAC856.625685E-410.053831
TAATACT1901.0004442E-109.9993814
Produced by FastQC (version 0.11.5)