##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062212_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2302752
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.292197770320033	32.0	32.0	32.0	32.0	32.0
2	30.896172058476118	32.0	32.0	32.0	32.0	32.0
3	30.917779248481818	32.0	32.0	32.0	32.0	32.0
4	30.925896492544574	32.0	32.0	32.0	32.0	32.0
5	30.858837165270078	32.0	32.0	32.0	32.0	32.0
6	34.50535185725602	36.0	36.0	36.0	32.0	36.0
7	34.45617244062756	36.0	36.0	36.0	32.0	36.0
8	34.424078667611624	36.0	36.0	36.0	32.0	36.0
9	34.545349216936955	36.0	36.0	36.0	32.0	36.0
10	34.280074015786326	36.0	36.0	36.0	32.0	36.0
11	34.5013501236781	36.0	36.0	36.0	32.0	36.0
12	34.37810541473854	36.0	36.0	36.0	32.0	36.0
13	34.43069553299704	36.0	36.0	36.0	32.0	36.0
14	34.355219320187324	36.0	36.0	36.0	32.0	36.0
15	34.321632550965106	36.0	36.0	36.0	32.0	36.0
16	34.31587161795973	36.0	36.0	36.0	32.0	36.0
17	34.26299749169689	36.0	36.0	36.0	32.0	36.0
18	34.2606033997582	36.0	36.0	36.0	32.0	36.0
19	34.2641291810842	36.0	36.0	36.0	32.0	36.0
20	34.248510043634745	36.0	36.0	36.0	32.0	36.0
21	34.24547367671378	36.0	36.0	36.0	32.0	36.0
22	34.20787171176054	36.0	36.0	36.0	32.0	36.0
23	34.177263552479815	36.0	36.0	36.0	32.0	36.0
24	34.16109898069788	36.0	36.0	36.0	32.0	36.0
25	33.81539609997082	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	35.0
5	165.0
6	483.0
7	108.0
8	397.0
9	300.0
10	149.0
11	53.0
12	96.0
13	101.0
14	340.0
15	491.0
16	777.0
17	1055.0
18	1408.0
19	1877.0
20	2735.0
21	3758.0
22	5733.0
23	8512.0
24	11941.0
25	17159.0
26	23625.0
27	30924.0
28	41628.0
29	55684.0
30	73270.0
31	101147.0
32	143331.0
33	205547.0
34	465792.0
35	1104129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.19600647926592	17.061053618965317	11.883590347109735	25.859349554659033
2	16.88945883693541	18.724269827649103	37.4920529594258	26.894218375989688
3	19.712047261978018	21.973050354382327	27.84792371171896	30.466978671920696
4	13.681683330213579	15.088092190724034	33.78000122473921	37.45022325432318
5	15.541685273966058	35.63153029326263	33.02931757403678	15.797466858734541
6	35.97928983155063	33.49237491227727	16.03934323649613	14.488992019675964
7	31.40337499989132	29.69274789747192	19.41985614212942	19.484020960507337
8	28.495940013581112	33.3657065697962	18.282937449516446	19.85541596710624
9	26.669165155472236	15.162059027232802	18.427065068387435	39.74171074890753
10	17.03055154540553	26.799219035360828	30.505626771954848	25.6646026472788
11	36.88905317402507	21.57950011415401	21.300051097509268	20.23139561431165
12	24.517737913281312	23.422829152054476	28.057537064053033	24.00189587061118
13	29.771877600749274	19.551155787110112	24.60916472520869	26.067801886931925
14	23.49650750974532	19.082657196842888	24.82854929267616	32.592286000735626
15	25.40745691486378	26.752550668763757	21.750009022951346	26.089983393421118
16	25.828548223774	25.864113178293902	23.09430756381366	25.213031034118444
17	24.419425205844206	26.111919593690036	24.58960511218004	24.879050088285716
18	24.901573433869867	25.518000236501997	25.120207356603274	24.460218973024862
19	25.63673011110855	25.198094536811528	24.88525535058462	24.2799200014953
20	25.883078057206543	24.67954652736666	24.74465867412892	24.69271674129788
21	26.0621281508295	24.426469923465575	24.618327578473	24.89307434723193
22	26.026909549016615	24.13525980351882	24.791023021049195	25.04680762641537
23	25.09025953282787	24.43841772550758	25.049796489581095	25.421526252083453
24	24.987840419358832	24.710919281682823	24.911644727262452	25.38959557169589
25	25.134175155298944	24.554227278022047	24.874343902180357	25.437253664498655
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	132.0
1	132.0
2	383.0
3	634.0
4	634.0
5	634.0
6	1272.0
7	1910.0
8	1910.0
9	1910.0
10	2451.5
11	2993.0
12	2993.0
13	2993.0
14	4122.5
15	5252.0
16	5252.0
17	5252.0
18	9114.5
19	12977.0
20	12977.0
21	12977.0
22	19612.0
23	26247.0
24	26247.0
25	26247.0
26	38372.0
27	50497.0
28	50497.0
29	50497.0
30	66749.0
31	83001.0
32	83001.0
33	83001.0
34	104601.5
35	126202.0
36	126202.0
37	126202.0
38	153120.0
39	180038.0
40	180038.0
41	180038.0
42	208790.5
43	237543.0
44	237543.0
45	237543.0
46	266987.0
47	296431.0
48	296431.0
49	296431.0
50	309797.5
51	323164.0
52	323164.0
53	323164.0
54	312296.5
55	301429.0
56	301429.0
57	301429.0
58	276602.0
59	251775.0
60	251775.0
61	251775.0
62	217807.5
63	183840.0
64	183840.0
65	183840.0
66	148277.0
67	112714.0
68	112714.0
69	112714.0
70	85216.5
71	57719.0
72	57719.0
73	57719.0
74	42636.0
75	27553.0
76	27553.0
77	27553.0
78	20135.5
79	12718.0
80	12718.0
81	12718.0
82	8693.0
83	4668.0
84	4668.0
85	4668.0
86	3176.5
87	1685.0
88	1685.0
89	1685.0
90	1159.5
91	634.0
92	634.0
93	634.0
94	409.0
95	184.0
96	184.0
97	184.0
98	498.0
99	812.0
100	812.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.553786078570337E-4
2	3.474104028571032E-4
3	5.645419046427926E-4
4	0.009597212378927475
5	0.034219924681424665
6	0.0637063826239213
7	0.10535220466641652
8	0.10847889829213046
9	0.11703387946248663
10	0.1316685426828421
11	0.140136671252484
12	0.13101714817748503
13	0.1274127652478426
14	0.11612192715498672
15	0.1329279053931991
16	0.11877093147677213
17	0.12302670891177166
18	0.111171328914273
19	0.09592869748891761
20	0.0914557885521324
21	0.09067411514570392
22	0.10331116854963104
23	0.08194542877391921
24	0.09106495184891816
25	0.08806853712427565
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2302752.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.51187698979113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.47847044292168	42.089181600581846
2	14.161012271823292	16.00530767112401
3	4.813137120991396	8.159982387493896
4	2.186996116821737	4.943650221239236
5	1.169290907068762	3.3039411952775586
6	0.7557657766725473	2.562584556264777
7	0.5055818259929514	1.999996457115136
8	0.3538200840030635	1.5996029650958952
9	0.26454970400358613	1.3455180297302685
>10	1.2283653567216275	11.8923275418917
>50	0.05364216236843267	2.0561877914921065
>100	0.026743821678436135	2.7863167275326224
>500	0.0023156549403493916	0.8492508056682758
>1k	3.0875399204631287E-4	0.40615204949261763
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3911	0.1698402606967663	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2484	0.10787093008713053	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.685260071427578E-5
2	0.0	0.0	0.0	0.0	8.685260071427578E-5
3	0.0	0.0	0.0	0.0	8.685260071427578E-5
4	0.0	0.0	0.0	0.0	8.685260071427578E-5
5	0.0	0.0	0.0	0.0	8.685260071427578E-5
6	0.0	0.0	0.0	0.0	2.1713150178568946E-4
7	0.0	0.0	0.0	0.0	2.1713150178568946E-4
8	0.0	0.0	0.0	0.0	2.1713150178568946E-4
9	0.0	0.0	0.0	0.0	2.1713150178568946E-4
10	0.0	0.0	0.0	0.0	2.1713150178568946E-4
11	0.0	0.0	0.0	0.0	2.1713150178568946E-4
12	0.0	0.0	0.0	0.0	3.0398410249996526E-4
13	0.0	4.342630035713789E-5	0.0	0.0	4.7768930392851685E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	810	0.0	16.294376	1
GTATCAA	2365	0.0	12.847741	1
TTAGTAC	70	1.0926339E-4	12.212735	3
CGTCGTA	120	9.998985E-9	11.876849	10
ACGGTAT	120	9.998985E-9	11.876849	9
TTCTACG	85	5.3344593E-5	11.174565	2
GTCTAGG	165	8.185452E-11	10.933963	1
CTAAGAC	140	8.374627E-9	10.855764	3
GTAGGAC	210	0.0	10.8557625	3
CTAGGAC	265	0.0	10.753351	3
TAGGACA	275	0.0	10.708428	4
CAAGACG	285	0.0	10.666007	4
TAAGACA	205	0.0	10.657878	4
TACGCTA	90	9.5037016E-5	10.557199	9
GTAATAC	135	5.7014404E-8	10.5542145	3
GCGTTAT	100	2.4129338E-5	10.444812	1
CGTTATT	110	6.060449E-6	10.36187	2
TCTAGGA	205	3.6379788E-12	10.193383	2
GTCTTAC	85	6.625685E-4	10.05383	1
TAATACT	190	1.0004442E-10	9.999381	4
>>END_MODULE