##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062212_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2302752
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.294410340323232	32.0	32.0	32.0	32.0	32.0
2	31.456441683689775	32.0	32.0	32.0	32.0	32.0
3	31.57499157529773	32.0	32.0	32.0	32.0	32.0
4	31.652511429802253	32.0	32.0	32.0	32.0	32.0
5	31.624131039729853	32.0	32.0	32.0	32.0	32.0
6	35.270069898973055	36.0	36.0	36.0	36.0	36.0
7	35.27927366907075	36.0	36.0	36.0	36.0	36.0
8	35.22532300482206	36.0	36.0	36.0	36.0	36.0
9	35.3142392233293	36.0	36.0	36.0	36.0	36.0
10	35.19992383026917	36.0	36.0	36.0	36.0	36.0
11	35.31180257361626	36.0	36.0	36.0	36.0	36.0
12	35.24503333402816	36.0	36.0	36.0	36.0	36.0
13	35.273007688192216	36.0	36.0	36.0	36.0	36.0
14	35.24183932963689	36.0	36.0	36.0	36.0	36.0
15	35.21778854171009	36.0	36.0	36.0	36.0	36.0
16	35.229480204555244	36.0	36.0	36.0	36.0	36.0
17	35.21598374466725	36.0	36.0	36.0	36.0	36.0
18	35.20613813385028	36.0	36.0	36.0	36.0	36.0
19	35.19983046372341	36.0	36.0	36.0	36.0	36.0
20	35.20445536471144	36.0	36.0	36.0	36.0	36.0
21	35.196044993121276	36.0	36.0	36.0	36.0	36.0
22	35.17896325787579	36.0	36.0	36.0	36.0	36.0
23	35.145698711802225	36.0	36.0	36.0	36.0	36.0
24	35.13506230805575	36.0	36.0	36.0	36.0	36.0
25	35.122754860271534	36.0	36.0	36.0	36.0	36.0
26	35.0856397041453	36.0	36.0	36.0	36.0	36.0
27	35.0692241283473	36.0	36.0	36.0	36.0	36.0
28	35.04906607398452	36.0	36.0	36.0	36.0	36.0
29	35.03749513625436	36.0	36.0	36.0	36.0	36.0
30	35.01748777115382	36.0	36.0	36.0	36.0	36.0
31	35.013635424049134	36.0	36.0	36.0	36.0	36.0
32	34.99701357332444	36.0	36.0	36.0	36.0	36.0
33	34.975861490946485	36.0	36.0	36.0	36.0	36.0
34	34.96704204360695	36.0	36.0	36.0	36.0	36.0
35	34.9581344408777	36.0	36.0	36.0	36.0	36.0
36	34.93330675643752	36.0	36.0	36.0	36.0	36.0
37	34.92382983491058	36.0	36.0	36.0	36.0	36.0
38	34.915734303998	36.0	36.0	36.0	36.0	36.0
39	34.90198901140896	36.0	36.0	36.0	36.0	36.0
40	34.883591459262654	36.0	36.0	36.0	32.0	36.0
41	34.859957129556285	36.0	36.0	36.0	32.0	36.0
42	34.83901045357902	36.0	36.0	36.0	32.0	36.0
43	34.8281369422326	36.0	36.0	36.0	32.0	36.0
44	34.79308236405831	36.0	36.0	36.0	32.0	36.0
45	34.77877904350968	36.0	36.0	36.0	32.0	36.0
46	34.75952729603535	36.0	36.0	36.0	32.0	36.0
47	34.738047345089704	36.0	36.0	36.0	32.0	36.0
48	34.71501446964328	36.0	36.0	36.0	32.0	36.0
49	34.70018699364934	36.0	36.0	36.0	32.0	36.0
50	34.29427441600311	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	7.0
20	28.0
21	60.0
22	157.0
23	406.0
24	1033.0
25	2506.0
26	5008.0
27	9608.0
28	16785.0
29	27221.0
30	40642.0
31	61010.0
32	92939.0
33	158538.0
34	364341.0
35	1522461.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.0176401748379	17.505423502335777	12.250077975063618	26.226858347762704
2	16.42087654094731	19.15399788590671	37.07402192785504	27.35110364529093
3	19.122240036686602	22.398412679112088	28.092522499205298	30.386824784996016
4	13.430023480610801	15.422336153583258	33.88968238424389	37.25795798156205
5	15.19620871027362	35.90529939828519	32.95886834535373	15.939623546087464
6	36.12392671712232	33.57901081664168	15.902955946547342	14.394106519688652
7	31.477162977168188	29.647743222023042	19.38237378580064	19.492720015008132
8	28.493776142632814	33.45171342810689	18.199224232570423	19.85528619668987
9	26.805361584747295	14.941860869081863	18.25915252706327	39.99362501910757
10	17.0049215047567	26.809932988846818	30.57699247552167	25.60815303087481
11	37.15875613179361	21.377030613804703	21.335558496963632	20.128654757438056
12	24.61806568835897	23.237977863009128	28.160435861091425	23.983520587540475
13	29.892580703436582	19.483861049735275	24.58271667986826	26.040841566959884
14	23.572881491363376	18.83481156459749	24.862295201567516	32.73001174247162
15	25.461751851697446	26.758634885563016	21.69273981740109	26.086873445338448
16	25.94787409042824	25.851206306248404	23.051879246043395	25.14904035727996
17	24.6136796320229	26.01461208154417	24.550537791303622	24.821170495129305
18	25.049603799288416	25.355803307962187	25.12529600516253	24.469296887586868
19	25.869394533149904	24.948235849974292	24.911280068370367	24.27108954850544
20	26.019553151472437	24.415904642668394	24.793757374338238	24.770784831520935
21	26.216795664659937	24.28514788194458	24.66934086172657	24.828715591668914
22	26.133556718222373	24.1196620391601	24.729019885771457	25.01776135684607
23	25.196547435416406	24.316947721682578	25.058842636983925	25.427662205917095
24	25.147271295765925	24.558576954327552	24.97200054022998	25.32215120967654
25	25.363424955867885	24.29348451521988	24.956779843187153	25.38631068572508
26	25.140701701969476	24.76940554399447	25.07013371872861	25.019759035307445
27	25.363805411114228	24.645704989964138	24.855498358044454	25.13499124087718
28	25.45565718424922	24.502348963268624	24.879121108186062	25.162872744296088
29	25.16239307331334	24.886244509815672	24.938660190919883	25.012702225951106
30	25.313953437875913	24.718312611552378	24.891976045823867	25.075757904747842
31	25.481708328592607	24.468568341568442	24.726217700463188	25.323505629375763
32	25.314310752812847	24.656571723127737	24.65791794720804	25.37119957685137
33	25.068765606340243	24.603148409510368	24.921680599283466	25.40640538486592
34	25.58557643076851	24.466948158512654	24.862391655820847	25.08508375489799
35	25.46037406627823	24.61520957360926	24.96131793896594	24.963098421146572
36	25.235306021593352	24.72604447038449	24.871349249982412	25.16730025803975
37	25.665639904998848	24.42032406178983	24.74554969820329	25.168486335008026
38	25.174747432637123	24.549321854893623	25.009119523074997	25.266811189394257
39	25.528164503228545	24.560577642537865	24.66671211978431	25.244545734449282
40	25.587678031778538	24.60559096974963	24.805829901565478	25.000901096906347
41	25.170016033185043	24.818519284117418	24.912234379804094	25.099230302893442
42	25.58244268510356	24.653765476867758	24.667141486039874	25.096650351988814
43	25.47160019438566	24.39839679602404	24.748082526179743	25.381920483410557
44	25.127190908178914	24.639576903216785	24.851203465933246	25.38202872267106
45	25.230794485425356	24.763125689951526	24.7948276326292	25.211252191993914
46	25.45153633092369	24.50664308848679	24.679701254269997	25.362119326319522
47	25.23528655626988	24.78408180919641	24.856648039452406	25.123983595081306
48	25.466710847512243	24.93840810854591	24.55637118337642	25.038509860565426
49	25.32030208410237	24.761689690663445	24.584981782018268	25.333026443215918
50	25.240531076984276	24.98422515663019	24.683927725378325	25.09131604100721
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	78.0
1	84.5
2	91.0
3	210.0
4	329.0
5	364.0
6	399.0
7	315.5
8	232.0
9	319.0
10	406.0
11	687.5
12	969.0
13	1828.0
14	2687.0
15	3391.0
16	4095.0
17	3873.0
18	3651.0
19	3458.5
20	3266.0
21	3877.5
22	4489.0
23	5726.0
24	6963.0
25	8848.5
26	10734.0
27	13349.5
28	15965.0
29	19219.0
30	22473.0
31	27077.0
32	31681.0
33	36909.0
34	42137.0
35	49339.5
36	56542.0
37	64564.5
38	72587.0
39	82069.0
40	91551.0
41	100361.0
42	109171.0
43	117887.0
44	126603.0
45	137594.0
46	148585.0
47	157815.0
48	167045.0
49	174545.0
50	182045.0
51	184500.0
52	186955.0
53	187173.5
54	187392.0
55	183780.5
56	180169.0
57	172039.5
58	163910.0
59	152253.5
60	140597.0
61	123922.5
62	107248.0
63	93141.5
64	79035.0
65	66245.5
66	53456.0
67	45203.5
68	36951.0
69	30778.5
70	24606.0
71	19391.5
72	14177.0
73	11935.0
74	9693.0
75	7569.5
76	5446.0
77	4591.0
78	3736.0
79	3019.5
80	2303.0
81	1733.5
82	1164.0
83	880.5
84	597.0
85	455.5
86	314.0
87	208.0
88	102.0
89	71.5
90	41.0
91	33.0
92	25.0
93	23.0
94	21.0
95	18.5
96	16.0
97	14.5
98	13.0
99	12.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03187490446213921
2	0.004776893039285168
3	1.737052014285516E-4
4	4.34263003571379E-5
5	0.0
6	1.737052014285516E-4
7	0.0
8	0.0
9	0.0
10	4.34263003571379E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	8.685260071427578E-4
17	0.0
18	2.1713150178568946E-4
19	0.0
20	1.302789010714137E-4
21	1.302789010714137E-4
22	0.0
23	0.0
24	0.001259362710356999
25	3.0398410249996526E-4
26	3.474104028571032E-4
27	4.342630035713789E-4
28	0.0014764942121426885
29	2.605578021428274E-4
30	9.553786078570337E-4
31	4.342630035713789E-4
32	6.513945053570685E-4
33	8.68526007142758E-5
34	9.119523074998958E-4
35	2.1713150178568946E-4
36	2.605578021428274E-4
37	0.0018673309153569293
38	0.0
39	5.645419046427926E-4
40	1.302789010714137E-4
41	0.001215936409999861
42	0.005298008643570823
43	0.004907171940356582
44	0.0048203193396423065
45	0.0023450202192854463
46	0.002518725420713998
47	0.001215936409999861
48	0.0041254985339281005
49	0.0035609566292853076
50	0.0015199205124998263
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2302752.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.643004029507075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.99039521268557	42.65028649389656
2	14.699639274086653	16.94662731816955
3	4.906823761051574	8.485321854911309
4	2.1705150765339987	5.004600372110207
5	1.2111814147842834	3.490806758643726
6	0.731839116588959	2.5311243087892956
7	0.4790379089511903	1.9329228881172074
8	0.33114291528559653	1.5270457920120286
9	0.253780390265459	1.3165797652813556
>10	1.1536877908585579	11.327317586782181
>50	0.04909348294767263	1.9204234768354442
>100	0.021422415372325254	2.241457733479964
>500	0.0013653858204998107	0.5497977333318366
>1k	7.585476780551414E-5	0.07568791763934364
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.0398410249996526E-4
2	0.0	0.0	0.0	0.0	3.0398410249996526E-4
3	0.0	0.0	0.0	0.0	3.0398410249996526E-4
4	0.0	0.0	0.0	0.0	3.4741040285710313E-4
5	0.0	0.0	0.0	0.0	3.4741040285710313E-4
6	0.0	0.0	0.0	0.0	3.4741040285710313E-4
7	0.0	0.0	0.0	0.0	3.9083670321424105E-4
8	0.0	0.0	0.0	0.0	4.342630035713789E-4
9	0.0	0.0	0.0	0.0	4.342630035713789E-4
10	0.0	0.0	0.0	0.0	4.7768930392851685E-4
11	0.0	0.0	0.0	0.0	4.7768930392851685E-4
12	0.0	0.0	0.0	0.0	9.119523074998958E-4
13	0.0	0.0	0.0	0.0	0.0010422312085713095
14	0.0	0.0	0.0	0.0	0.0010422312085713095
15	0.0	0.0	0.0	1.302789010714137E-4	0.0011725101096427232
16	0.0	0.0	0.0	1.7370520142855156E-4	0.0011725101096427232
17	0.0	0.0	0.0	1.7370520142855156E-4	0.0011725101096427232
18	0.0	0.0	0.0	2.1713150178568946E-4	0.001215936409999861
19	0.0	0.0	0.0	3.4741040285710313E-4	0.0013027890107141368
20	0.0	0.0	0.0	4.342630035713789E-4	0.0014330679117855506
21	0.0	0.0	0.0	6.079682049999305E-4	0.0014330679117855506
22	0.0	0.0	0.0	6.513945053570684E-4	0.0014330679117855506
23	0.0	0.0	0.0	6.948208057142063E-4	0.0014764942121426885
24	0.0	0.0	0.0	9.988049082141715E-4	0.0015199205124998263
25	0.0	0.0	0.0	0.001259362710356999	0.0015199205124998263
26	0.0	0.0	0.0	0.0016936257139283778	0.00165019941357124
27	0.0	0.0	0.0	0.0019541835160712053	0.0016936257139283778
28	0.0	0.0	0.0	0.002518725420713998	0.0017370520142855157
29	0.0	0.0	0.0	0.0034306777282138937	0.0017370520142855157
30	0.0	0.0	0.0	0.005688845346785064	0.0017370520142855157
31	0.0	0.0	0.0	0.010769722488570197	0.0017804783146426536
32	0.0	0.0	0.0	0.01689283083892664	0.0018239046149997917
33	0.0	0.0	0.0	0.022538249885354567	0.0018239046149997917
34	0.0	0.0	0.0	0.030224705048567974	0.0018239046149997917
35	0.0	0.0	0.0	0.0383888495157099	0.0018239046149997917
36	0.0	0.0	0.0	0.047334667389280306	0.0018239046149997917
37	0.0	4.342630035713789E-5	0.0	0.06396694042606411	0.0018239046149997917
38	0.0	4.342630035713789E-5	0.0	0.08715658481677575	0.0019107572157140674
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1095	0.0	31.753788	1
GTATCAA	2575	0.0	25.125961	1
ATACCGT	300	0.0	21.266102	6
TACCGTA	95	1.5985002E-5	18.525822	7
ATTATAC	240	0.0	17.416204	3
CGTCGTA	255	0.0	17.254442	10
TACCGTC	310	0.0	17.031803	7
TATCAAC	3785	0.0	16.97328	2
TCTAGCG	185	4.2382453E-10	16.648205	28
TCAACGC	3845	0.0	16.420889	4
ATCAACG	3885	0.0	16.308447	3
GTATAAA	360	0.0	15.893642	1
CAACGCA	3975	0.0	15.883854	5
AACGCAG	4100	0.0	15.560561	6
CGCAATA	205	1.8117134E-9	15.024642	36
ACCGTCG	295	0.0	14.914858	8
GTATATA	400	0.0	14.304277	1
TATACTG	480	0.0	14.207955	5
CTTACAC	390	0.0	14.102189	3
TACGCTA	190	1.7554885E-7	13.894366	9
>>END_MODULE