FastQCFastQC Report
Thu 2 Feb 2017
SRR4062211_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062211_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621058
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26820.16544750403748662No Hit
GCGCAAGACGGACCAGAGCGAAAGC19050.1175158445903848No Hit
TATCAACGCAGAGTACTTTTTTTTT18000.1110385933137494No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATATCG400.005277748714.2506025
GGTATCA8050.014.1568411
GACTGCG551.9585012E-413.819627
GTAATAC1153.783498E-1013.21713353
CCTAATG550.003073251812.0884322
AGGACGT2800.011.8755035
TAGGACG2800.011.8751354
CCGACCA3300.011.8046239
GTCTTAG1459.822543E-1111.7892311
TAGAACA3550.011.7747814
CGACCAT3150.011.76349310
GTATAAT1302.6284397E-911.6884691
CAAGACG3500.011.6715614
GACCATA3100.011.64673311
GTCCTAG1552.5465852E-1111.6413371
TTAGGAC2450.011.6324263
CGTCGTA2300.011.56677910
GTCTTAT1657.2759576E-1211.5113711
TCGCGTA1251.8279025E-811.4015389
ACCGTCC752.0719127E-411.4011878
Produced by FastQC (version 0.11.5)