##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062211_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1621058
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24653775497237	32.0	32.0	32.0	32.0	32.0
2	30.748153366505086	32.0	32.0	32.0	32.0	32.0
3	30.775144381015362	32.0	32.0	32.0	32.0	32.0
4	30.78711680889888	32.0	32.0	32.0	32.0	32.0
5	30.68804139025254	32.0	32.0	32.0	32.0	32.0
6	34.35595765234803	36.0	36.0	36.0	32.0	36.0
7	34.28956027483286	36.0	36.0	36.0	32.0	36.0
8	34.23871261854912	36.0	36.0	36.0	32.0	36.0
9	34.39576498805101	36.0	36.0	36.0	32.0	36.0
10	34.04420384711713	36.0	36.0	36.0	32.0	36.0
11	34.37634803936688	36.0	36.0	36.0	32.0	36.0
12	34.17415107911006	36.0	36.0	36.0	32.0	36.0
13	34.28075121309663	36.0	36.0	36.0	32.0	36.0
14	34.15904551225187	36.0	36.0	36.0	32.0	36.0
15	34.110786905835575	36.0	36.0	36.0	32.0	36.0
16	34.116270978583124	36.0	36.0	36.0	32.0	36.0
17	34.04402063343816	36.0	36.0	36.0	32.0	36.0
18	34.056547020526104	36.0	36.0	36.0	32.0	36.0
19	34.054447157350324	36.0	36.0	36.0	32.0	36.0
20	34.046470885063954	36.0	36.0	36.0	32.0	36.0
21	34.02647653569459	36.0	36.0	36.0	32.0	36.0
22	33.991458664649876	36.0	36.0	36.0	32.0	36.0
23	33.95854805935383	36.0	36.0	36.0	32.0	36.0
24	33.93548596040364	36.0	36.0	36.0	32.0	36.0
25	33.5716618406004	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	16.0
5	106.0
6	325.0
7	80.0
8	244.0
9	232.0
10	107.0
11	40.0
12	68.0
13	68.0
14	268.0
15	389.0
16	551.0
17	725.0
18	1063.0
19	1466.0
20	2216.0
21	3269.0
22	4977.0
23	7327.0
24	10384.0
25	14678.0
26	19835.0
27	25801.0
28	33265.0
29	43705.0
30	57178.0
31	77841.0
32	109029.0
33	154336.0
34	334909.0
35	716559.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.70560700388145	17.08226303542655	11.287232178768743	25.924897781923256
2	17.00644518141261	18.903912017845155	37.4647760472186	26.62486675352363
3	19.75724391151173	22.21626586702084	27.75853443229115	30.26795578917628
4	13.143456804899461	15.071999545934913	34.36310276985871	37.421440879306914
5	15.273296321596083	36.08033484576694	33.021035230262	15.625333602374972
6	35.78898444381212	34.126954244945	16.020472469133594	14.063588842109292
7	31.519253652725965	29.637206705911485	19.537830823258144	19.305708818104407
8	28.467702742841546	33.44763640877151	18.426260930887707	19.65839991749924
9	26.724035710933137	14.664987673107612	18.369292945262668	40.24168367069658
10	16.919660780353425	26.484703615172233	30.41142426544617	26.184211339028174
11	37.58025521064329	21.199235529353917	21.36212411158702	19.858385148415774
12	24.590271266548815	23.423747280725486	28.20818802740955	23.77779342531615
13	29.766523747231478	19.258363010424347	24.722282955776503	26.252830286567676
14	23.42658854160234	19.415371238152172	24.536585305604206	32.62145491464128
15	25.24147353519232	27.25794277765905	21.59975985443028	25.900823832718352
16	25.92771691502745	25.79438086433134	23.27748535248827	25.000416868152943
17	24.305739549114392	26.224378060597754	24.65381933572098	24.816063054566882
18	24.972859413615126	25.10297366961724	25.607374829097672	24.31679208766996
19	25.729970837596344	24.987542843664695	24.808974833148202	24.47351148559076
20	25.93637861159613	24.40530141191521	24.508225264317343	25.150094712171317
21	26.533923577024947	24.26278192471306	24.240308210931858	24.962986287330136
22	26.173940910699166	24.2780248734008	24.67006405138436	24.87797016451567
23	24.706583240471787	24.44877283853821	25.202016304532364	25.642627616457638
24	24.893125981685092	24.7777316776813	25.153797330804412	25.175345009829197
25	25.024680054502944	24.502864368051693	25.005047140601917	25.467408436843446
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	550.0
1	550.0
2	511.5
3	473.0
4	473.0
5	473.0
6	1010.5
7	1548.0
8	1548.0
9	1548.0
10	1877.5
11	2207.0
12	2207.0
13	2207.0
14	3025.5
15	3844.0
16	3844.0
17	3844.0
18	6358.5
19	8873.0
20	8873.0
21	8873.0
22	14283.5
23	19694.0
24	19694.0
25	19694.0
26	29572.0
27	39450.0
28	39450.0
29	39450.0
30	50481.0
31	61512.0
32	61512.0
33	61512.0
34	77112.5
35	92713.0
36	92713.0
37	92713.0
38	110161.5
39	127610.0
40	127610.0
41	127610.0
42	147266.0
43	166922.0
44	166922.0
45	166922.0
46	185966.5
47	205011.0
48	205011.0
49	205011.0
50	213524.0
51	222037.0
52	222037.0
53	222037.0
54	210643.0
55	199249.0
56	199249.0
57	199249.0
58	185380.0
59	171511.0
60	171511.0
61	171511.0
62	150205.5
63	128900.0
64	128900.0
65	128900.0
66	106053.5
67	83207.0
68	83207.0
69	83207.0
70	62941.0
71	42675.0
72	42675.0
73	42675.0
74	32294.0
75	21913.0
76	21913.0
77	21913.0
78	17343.5
79	12774.0
80	12774.0
81	12774.0
82	8729.0
83	4684.0
84	4684.0
85	4684.0
86	3434.0
87	2184.0
88	2184.0
89	2184.0
90	1450.0
91	716.0
92	716.0
93	716.0
94	451.5
95	187.0
96	187.0
97	187.0
98	400.5
99	614.0
100	614.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.636335035513843E-4
2	1.2337621479305492E-4
3	4.3181675177569217E-4
4	0.008944775572496481
5	0.03028886073169498
6	0.05743162798616706
7	0.10184706531166682
8	0.1035743323187696
9	0.11313598896523136
10	0.12633724394808823
11	0.1332463119764993
12	0.12707750123684655
13	0.12115544292677992
14	0.1094347025214397
15	0.125843739088916
16	0.11356780571700703
17	0.11652883487204037
18	0.105610039862855
19	0.09296397784656686
20	0.08722698385868982
21	0.08562309306638011
22	0.0972821453643238
23	0.07791207964181417
24	0.08599322171075928
25	0.08198349472998498
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1621058.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.41265468604792
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.78982280421609	42.551279344528346
2	13.70120402735553	15.184401751018822
3	4.242091529638091	7.051966592353331
4	1.7877157958938763	3.962483122986428
5	0.9409640608566009	2.6070658288114106
6	0.5690408691265912	1.8919239109896235
7	0.37953825708382594	1.472185566595137
8	0.2617423904386578	1.1603072558462464
9	0.18814852422314557	0.9383228282220021
>10	1.0094878343021783	9.834854626267557
>50	0.06369726000359562	2.4418530685026427
>100	0.05828440218505453	6.838666022773005
>500	0.005917553619809845	2.266334779764606
>1k	0.0023446910569057877	1.7983553013408804
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2682	0.16544750403748662	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1905	0.1175158445903848	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1800	0.1110385933137494	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4675242958610983E-4
2	0.0	0.0	0.0	0.0	2.4675242958610983E-4
3	0.0	0.0	0.0	0.0	2.4675242958610983E-4
4	0.0	0.0	0.0	0.0	2.4675242958610983E-4
5	0.0	0.0	0.0	0.0	2.4675242958610983E-4
6	0.0	0.0	0.0	0.0	2.4675242958610983E-4
7	0.0	0.0	0.0	0.0	2.4675242958610983E-4
8	0.0	0.0	0.0	0.0	2.4675242958610983E-4
9	0.0	0.0	0.0	0.0	2.4675242958610983E-4
10	0.0	0.0	0.0	0.0	2.4675242958610983E-4
11	0.0	0.0	0.0	0.0	2.4675242958610983E-4
12	0.0	0.0	0.0	6.168810739652746E-5	4.3181675177569217E-4
13	0.0	0.0	0.0	6.168810739652746E-5	4.3181675177569217E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATATCG	40	0.0052777487	14.250602	5
GGTATCA	805	0.0	14.156841	1
GACTGCG	55	1.9585012E-4	13.81962	7
GTAATAC	115	3.783498E-10	13.2171335	3
CCTAATG	55	0.0030732518	12.088432	2
AGGACGT	280	0.0	11.875503	5
TAGGACG	280	0.0	11.875135	4
CCGACCA	330	0.0	11.804623	9
GTCTTAG	145	9.822543E-11	11.789231	1
TAGAACA	355	0.0	11.774781	4
CGACCAT	315	0.0	11.763493	10
GTATAAT	130	2.6284397E-9	11.688469	1
CAAGACG	350	0.0	11.671561	4
GACCATA	310	0.0	11.646733	11
GTCCTAG	155	2.5465852E-11	11.641337	1
TTAGGAC	245	0.0	11.632426	3
CGTCGTA	230	0.0	11.566779	10
GTCTTAT	165	7.2759576E-12	11.511371	1
TCGCGTA	125	1.8279025E-8	11.401538	9
ACCGTCC	75	2.0719127E-4	11.401187	8
>>END_MODULE