FastQCFastQC Report
Thu 2 Feb 2017
SRR4062211_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062211_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621058
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18290.11282754842824871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18500.026.8823091
GGTATCA8350.026.8808841
TCGCGTG450.001398921624.44293613
GCGTACG550.004477860420.0049361
ATACCGT6100.019.4742076
CGTCGTA5750.018.7466710
ACCGTCG6150.018.600488
GCGTATT600.007415802718.3322039
TACCGTC6400.018.2176257
TATACCG851.4318903E-418.116535
CCGTCGT6300.017.4592429
TCAACGC28350.017.226454
CGCAATA3500.016.97195236
GTCCTAT4300.016.887891
CAACGCA29100.016.782475
ATCAACG29250.016.7716143
TATCAAC29650.016.7684562
GTGTACG1053.7676426E-516.7660431
TAGGACG2900.016.6886254
AATACCG800.00198956516.4989815
Produced by FastQC (version 0.11.5)