##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062211_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1621058
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28202939068189	32.0	32.0	32.0	32.0	32.0
2	31.469532243756856	32.0	32.0	32.0	32.0	32.0
3	31.585205464579307	32.0	32.0	32.0	32.0	32.0
4	31.665345718660284	32.0	32.0	32.0	32.0	32.0
5	31.641320051472558	32.0	32.0	32.0	32.0	32.0
6	35.29525100274019	36.0	36.0	36.0	36.0	36.0
7	35.29853897886442	36.0	36.0	36.0	36.0	36.0
8	35.25153510855256	36.0	36.0	36.0	36.0	36.0
9	35.327029014384436	36.0	36.0	36.0	36.0	36.0
10	35.21495035957998	36.0	36.0	36.0	36.0	36.0
11	35.33031884115189	36.0	36.0	36.0	36.0	36.0
12	35.25738992682557	36.0	36.0	36.0	36.0	36.0
13	35.28690336804729	36.0	36.0	36.0	36.0	36.0
14	35.250607936298394	36.0	36.0	36.0	36.0	36.0
15	35.234410489939286	36.0	36.0	36.0	36.0	36.0
16	35.24695785098374	36.0	36.0	36.0	36.0	36.0
17	35.226531067981526	36.0	36.0	36.0	36.0	36.0
18	35.22477850885039	36.0	36.0	36.0	36.0	36.0
19	35.216900937535854	36.0	36.0	36.0	36.0	36.0
20	35.20845953691972	36.0	36.0	36.0	36.0	36.0
21	35.20866495831734	36.0	36.0	36.0	36.0	36.0
22	35.18542458073678	36.0	36.0	36.0	36.0	36.0
23	35.154626793119064	36.0	36.0	36.0	36.0	36.0
24	35.14158962850188	36.0	36.0	36.0	36.0	36.0
25	35.12142008490751	36.0	36.0	36.0	36.0	36.0
26	35.09011892233344	36.0	36.0	36.0	36.0	36.0
27	35.07165752243288	36.0	36.0	36.0	36.0	36.0
28	35.05337255051948	36.0	36.0	36.0	36.0	36.0
29	35.03353612270505	36.0	36.0	36.0	36.0	36.0
30	35.016640366970215	36.0	36.0	36.0	36.0	36.0
31	35.013857616445556	36.0	36.0	36.0	36.0	36.0
32	34.99324515224008	36.0	36.0	36.0	36.0	36.0
33	34.97235817595669	36.0	36.0	36.0	36.0	36.0
34	34.96444174113449	36.0	36.0	36.0	36.0	36.0
35	34.95039350843708	36.0	36.0	36.0	36.0	36.0
36	34.92803342014906	36.0	36.0	36.0	36.0	36.0
37	34.919816564243845	36.0	36.0	36.0	36.0	36.0
38	34.894755770614005	36.0	36.0	36.0	32.0	36.0
39	34.87304772562117	36.0	36.0	36.0	32.0	36.0
40	34.863730353879994	36.0	36.0	36.0	32.0	36.0
41	34.84283782566694	36.0	36.0	36.0	32.0	36.0
42	34.8111757876646	36.0	36.0	36.0	32.0	36.0
43	34.81122945631803	36.0	36.0	36.0	32.0	36.0
44	34.75935654368937	36.0	36.0	36.0	32.0	36.0
45	34.746390937276765	36.0	36.0	36.0	32.0	36.0
46	34.71552961090843	36.0	36.0	36.0	32.0	36.0
47	34.70431964803233	36.0	36.0	36.0	32.0	36.0
48	34.669513984077064	36.0	36.0	36.0	32.0	36.0
49	34.6565095141568	36.0	36.0	36.0	32.0	36.0
50	34.25737327103657	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	4.0
20	9.0
21	36.0
22	120.0
23	317.0
24	803.0
25	1825.0
26	3726.0
27	7117.0
28	12320.0
29	19442.0
30	28643.0
31	42617.0
32	64458.0
33	109776.0
34	256353.0
35	1073490.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.35323128265192	17.579243962673292	11.741793592355949	26.32573116231884
2	16.439521526968086	19.26834835501761	37.250198952492	27.041931165522303
3	19.11730051509824	22.567533388852905	28.119305388482772	30.195860707566087
4	12.89898325661389	15.443494310505853	34.51671686022338	37.14080557265687
5	14.864930721128253	36.380645467741104	32.91747298213449	15.836950828996144
6	35.971574067505415	34.22505713871522	15.971944196834778	13.831424596944583
7	31.5053502095545	29.589626034355344	19.59800327933979	19.30702047675037
8	28.527295136879744	33.40003874013144	18.448322021790705	19.62434410119811
9	26.90199857130343	14.371910196920776	18.377627450714286	40.348463781061504
10	16.871327244305878	26.466295468761757	30.514515828551474	26.147861458380884
11	37.81696891783021	21.023738817488333	21.401886915829042	19.757405348852416
12	24.62657104187512	23.28115341955686	28.286834894248074	23.805440644319944
13	29.873329640271972	19.072790732965757	24.90182337707843	26.15205624968385
14	23.561525867674074	19.26365373725061	24.603808130245802	32.57101226482951
15	25.36466924687457	27.103225177630907	21.65567178965836	25.876433785836163
16	25.9984898658152	25.700102649643934	23.368216116981113	24.93319136755975
17	24.422938599359185	26.049468927083424	24.71626555002967	24.811326923527723
18	25.117176559999706	24.93476482642817	25.710492776939503	24.23756583663262
19	25.897901247210154	24.76993420346465	24.824528178510576	24.507636370814616
20	26.001909868517643	24.22016786609206	24.67308306817663	25.104839197213664
21	26.590181591393762	24.088542228928407	24.399450482000322	24.9218256976775
22	26.178828888293943	24.11320261212122	24.87141114013194	24.8365573594529
23	24.67846596387419	24.2267853628836	25.394048451103203	25.700700222139012
24	25.001619331798924	24.64585984667858	25.20772171428325	25.144799107239248
25	25.186298314185322	24.265293734454577	25.154220458762683	25.39418749259742
26	24.92185644533961	25.211544333782633	25.330479224949183	24.536119995928576
27	25.27349489899781	24.583264962178927	24.97220943904398	25.17103069977928
28	25.000956177461603	24.540695529654457	25.331670201438833	25.126678091445108
29	24.786034271529513	24.730329773098244	25.387679867542968	25.095956087829276
30	24.75500205424439	24.881341168661482	25.424204550922436	24.939452226171692
31	25.325929919669548	24.552605897150865	24.69640123031065	25.425062952868938
32	25.345270467006532	24.59686573130469	24.61117746050703	25.446686341181756
33	24.812329239502372	24.459781488251185	25.172834761516715	25.55505451072973
34	25.504504187725587	24.649439528897066	25.15127568787333	24.694780595504017
35	25.681978711394738	24.392818257246052	25.42640440947408	24.498798621885133
36	24.534331037503353	25.008590084852152	25.123206800509546	25.33387207713495
37	25.610103662102507	24.636837183134734	24.86224858670628	24.890810568056477
38	24.925033481240945	24.512154723738895	25.183074993661542	25.37973680135862
39	25.741203061971675	24.333470898746302	24.668501283429784	25.256824755852236
40	25.810413588681445	24.560486843444547	24.939129147376246	24.68997042049776
41	24.765798788680367	25.031985599386076	25.435059671495246	24.76715594043831
42	25.800037754798623	24.91569945699716	24.862645328213816	24.4216174599904
43	25.310165584097117	24.313551788445203	25.136012644132688	25.240269983324993
44	24.843290114861976	24.891779031196386	24.854271065380455	25.41065978856118
45	24.977313876628205	25.043937977896835	24.894157128007723	25.084591017467233
46	24.8854271474583	24.71504070582676	24.91627190517581	25.483260241539128
47	25.15630388127136	24.609309696670227	25.21250233646157	25.021884085596845
48	25.656859400533865	25.060317370230518	24.32201178401847	24.960811445217146
49	25.09901295496607	25.13491671807526	24.461505243676744	25.304565083281926
50	25.0629072624499	25.30842911050149	24.602584641806448	25.02607898524216
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	257.0
1	161.5
2	66.0
3	183.0
4	300.0
5	289.0
6	278.0
7	233.0
8	188.0
9	257.0
10	326.0
11	559.0
12	792.0
13	1438.5
14	2085.0
15	2616.0
16	3147.0
17	3112.0
18	3077.0
19	2927.0
20	2777.0
21	3273.0
22	3769.0
23	4593.5
24	5418.0
25	6869.5
26	8321.0
27	10527.5
28	12734.0
29	15193.5
30	17653.0
31	20692.5
32	23732.0
33	27780.5
34	31829.0
35	36797.0
36	41765.0
37	48771.5
38	55778.0
39	61375.0
40	66972.0
41	73165.0
42	79358.0
43	83347.0
44	87336.0
45	94948.5
46	102561.0
47	110002.5
48	117444.0
49	122994.5
50	128545.0
51	125754.0
52	122963.0
53	123196.0
54	123429.0
55	123524.5
56	123620.0
57	118910.5
58	114201.0
59	105378.0
60	96555.0
61	85131.0
62	73707.0
63	63582.5
64	53458.0
65	45187.0
66	36916.0
67	32003.5
68	27091.0
69	23762.5
70	20434.0
71	15939.0
72	11444.0
73	10065.5
74	8687.0
75	6401.0
76	4115.0
77	3614.0
78	3113.0
79	2581.0
80	2049.0
81	1629.5
82	1210.0
83	998.0
84	786.0
85	643.0
86	500.0
87	328.0
88	156.0
89	102.0
90	48.0
91	32.0
92	16.0
93	17.0
94	18.0
95	16.0
96	14.0
97	13.0
98	12.0
99	10.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03429858771246926
2	0.0041947913029638665
3	4.935048591722197E-4
4	0.0
5	6.168810739652746E-5
6	1.8506432218958236E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.168810739652744E-4
17	0.0
18	0.0
19	0.0
20	2.4675242958610983E-4
21	6.168810739652746E-5
22	0.0
23	6.168810739652746E-5
24	0.0011720740405340216
25	1.2337621479305492E-4
26	1.8506432218958236E-4
27	2.4675242958610983E-4
28	0.001233762147930549
29	2.4675242958610983E-4
30	0.001480514577516659
31	2.4675242958610983E-4
32	6.168810739652744E-4
33	6.168810739652746E-5
34	6.78569181361802E-4
35	1.8506432218958236E-4
36	1.8506432218958236E-4
37	0.001357138362723604
38	6.168810739652746E-5
39	6.78569181361802E-4
40	1.8506432218958236E-4
41	9.870097183444393E-4
42	0.0044415437325499765
43	0.004256479410360394
44	0.004256479410360394
45	0.0014188264701201314
46	0.002282459973671516
47	0.0010486978257409666
48	0.003022717262429845
49	0.003577910228998592
50	0.0012954502553270766
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1621058.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.90914283454869
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.59859112077761	43.59160163017526
2	14.03546729931859	15.974928265731178
3	4.3087931021420545	7.356291662829609
4	1.7254619896191061	3.9277825129127306
5	0.9182064385023558	2.6127170680166407
6	0.5549326809251937	1.8948445921398525
7	0.36588053015478217	1.4575363145671227
8	0.24939068359252234	1.1354088027338072
9	0.18419081680512484	0.9433927352137534
>10	0.943347950773934	9.360681902432097
>50	0.0591002782883524	2.3266872991427894
>100	0.04923099913006528	5.857528079749617
>500	0.005554582477744641	2.1671271826342644
>1k	0.0018515274925815473	1.3934719517213
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1829	0.11282754842824871	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.168810739652746E-5
7	6.168810739652746E-5	0.0	0.0	0.0	6.168810739652746E-5
8	6.168810739652746E-5	0.0	0.0	0.0	6.168810739652746E-5
9	6.168810739652746E-5	0.0	0.0	0.0	6.168810739652746E-5
10	6.168810739652746E-5	0.0	0.0	0.0	6.168810739652746E-5
11	6.168810739652746E-5	0.0	0.0	0.0	6.168810739652746E-5
12	6.168810739652746E-5	0.0	0.0	6.168810739652746E-5	4.3181675177569217E-4
13	6.168810739652746E-5	0.0	0.0	6.168810739652746E-5	4.935048591722197E-4
14	6.168810739652746E-5	0.0	0.0	6.168810739652746E-5	4.935048591722197E-4
15	6.168810739652746E-5	0.0	0.0	3.084405369826373E-4	6.168810739652746E-4
16	6.168810739652746E-5	0.0	0.0	3.084405369826373E-4	6.78569181361802E-4
17	6.168810739652746E-5	0.0	0.0	3.084405369826373E-4	6.78569181361802E-4
18	6.168810739652746E-5	0.0	0.0	3.084405369826373E-4	6.78569181361802E-4
19	6.168810739652746E-5	0.0	0.0	4.935048591722197E-4	6.78569181361802E-4
20	6.168810739652746E-5	0.0	0.0	5.551929665687471E-4	7.402572887583294E-4
21	6.168810739652746E-5	0.0	0.0	6.168810739652746E-4	7.402572887583294E-4
22	6.168810739652746E-5	0.0	0.0	8.019453961548568E-4	8.019453961548568E-4
23	6.168810739652746E-5	0.0	0.0	0.0010486978257409666	8.636335035513843E-4
24	6.168810739652746E-5	0.0	0.0	0.0014188264701201314	9.253216109479118E-4
25	6.168810739652746E-5	0.0	0.0	0.0022207718662749882	9.253216109479118E-4
26	6.168810739652746E-5	0.0	0.0	0.002961029155033318	9.253216109479118E-4
27	6.168810739652746E-5	0.0	0.0	0.0033311577994124823	9.253216109479118E-4
28	6.168810739652746E-5	0.0	0.0	0.0041947913029638665	9.253216109479118E-4
29	6.168810739652746E-5	0.0	0.0	0.006168810739652746	9.253216109479118E-4
30	6.168810739652746E-5	0.0	0.0	0.009068151787289536	9.253216109479118E-4
31	6.168810739652746E-5	0.0	0.0	0.015051898204752698	9.253216109479118E-4
32	6.168810739652746E-5	0.0	0.0	0.021405773266595027	0.0010486978257409666
33	6.168810739652746E-5	0.0	0.0	0.030103796409505397	0.0010486978257409666
34	6.168810739652746E-5	0.0	0.0	0.03880181955241577	0.0010486978257409666
35	6.168810739652746E-5	0.0	0.0	0.050275807528169875	0.0010486978257409666
36	6.168810739652746E-5	0.0	0.0	0.06304524575925105	0.0010486978257409666
37	6.168810739652746E-5	6.168810739652746E-5	0.0	0.08303219255572596	0.0010486978257409666
38	6.168810739652746E-5	6.168810739652746E-5	0.0	0.10727561876256124	0.0011103859331374941
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1850	0.0	26.882309	1
GGTATCA	835	0.0	26.880884	1
TCGCGTG	45	0.0013989216	24.442936	13
GCGTACG	55	0.0044778604	20.004936	1
ATACCGT	610	0.0	19.474207	6
CGTCGTA	575	0.0	18.74667	10
ACCGTCG	615	0.0	18.60048	8
GCGTATT	60	0.0074158027	18.332203	9
TACCGTC	640	0.0	18.217625	7
TATACCG	85	1.4318903E-4	18.11653	5
CCGTCGT	630	0.0	17.459242	9
TCAACGC	2835	0.0	17.22645	4
CGCAATA	350	0.0	16.971952	36
GTCCTAT	430	0.0	16.88789	1
CAACGCA	2910	0.0	16.78247	5
ATCAACG	2925	0.0	16.771614	3
TATCAAC	2965	0.0	16.768456	2
GTGTACG	105	3.7676426E-5	16.766043	1
TAGGACG	290	0.0	16.688625	4
AATACCG	80	0.001989565	16.498981	5
>>END_MODULE