FastQCFastQC Report
Thu 2 Feb 2017
SRR4062210_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062210_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1045925
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17930.17142720558357435No Hit
TATCAACGCAGAGTACTTTTTTTTT12770.12209288428902645No Hit
GCGCAAGACGGACCAGAGCGAAAGC12130.1159738987021058No Hit
GATTAAGAGGGACGGCCGGGGGCAT10520.10058082558500848No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG400.005275979614.2506475
GTTTAGA707.2824423E-613.5668481
GGTATCA4550.013.3581261
AACCGCG500.001498460713.3006047
TCTTAAG500.001500625213.2980562
TTTTCGG957.3732735E-813.0005916
TCGCCAG1850.012.83719117
CGAACGA604.0884624E-412.66724116
TTAGCCT751.477522E-512.6666354
GTATAGG604.101695E-412.6623911
GTATTAC1001.447006E-712.345831
CGCCAGT1700.012.2923218
ATCGCCA1800.012.1394416
GTATCAA12150.012.03708651
GCGTTAT951.0429212E-611.9959491
CGTTTAT802.8741657E-511.8709911
TGTGCTA658.013282E-411.69339810
ATAGCGT1406.7666406E-1011.5367916
AGCGTAT1501.7644197E-1011.40051658
CGTATAT1604.5474735E-1111.28230310
Produced by FastQC (version 0.11.5)