FastQCFastQC Report
Thu 2 Feb 2017
SRR4062210_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062210_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1045925
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT12320.11779047254822286No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11220.10727346607070298No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10930.10450080072662955No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10800.10325788177928628No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10530.10067643473480412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13450.027.1568721
GGTATCA6200.023.7780951
CGGATCG752.0625976E-623.46827326
ATACGAA2200.021.00143640
CGAATGC2350.019.66092143
TACGAAT2550.018.11888741
GTATTAA1355.512993E-817.9288671
TCAACGC20200.017.6422424
CGCAATA2800.017.28524636
TAATACC1408.3760824E-817.284424
CAACGCA20750.017.2806325
AACGCAG20750.017.2806326
ATCAACG20650.017.2577883
GTCCTAA902.2122369E-417.1139181
TCGATTT902.2138536E-417.11228430
TCTAGCG2700.017.11228228
TATCAAC21550.016.844092
GTTATAG1702.4610927E-916.826291
CAATACG2750.016.80115138
CTAGCGG2500.016.72114429
Produced by FastQC (version 0.11.5)